Insight into the complex genetic network of tetraploid Atlantic salmon ( Salmo salar L.): Description of multiple novel Pax -7 splice variants
Paired box transcription factor 7 (Pax-7) cDNA was isolated from the skeletal muscle and brain of alevin and adult stages of Atlantic salmon, identifying 10 variants categorised as novel or established insertions (ins) or deletions (del). Two putative Pax-7 paralogs were identified (denoted Pax-7 al...
Published in: | Gene |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2006
|
Subjects: | |
Online Access: | https://research-portal.st-andrews.ac.uk/en/researchoutput/insight-into-the-complex-genetic-network-of-tetraploid-atlantic-salmon-salmo-salar-l-description-of-multiple-novel-pax-7-splice-variants(7ddb9309-333c-4070-bc7c-d494e226b0e8).html https://doi.org/10.1016/j.gene.2005.12.026 http://www.scopus.com/inward/record.url?scp=33646271823&partnerID=8YFLogxK |
id |
ftunstandrewcris:oai:research-portal.st-andrews.ac.uk:publications/7ddb9309-333c-4070-bc7c-d494e226b0e8 |
---|---|
record_format |
openpolar |
spelling |
ftunstandrewcris:oai:research-portal.st-andrews.ac.uk:publications/7ddb9309-333c-4070-bc7c-d494e226b0e8 2024-06-23T07:51:12+00:00 Insight into the complex genetic network of tetraploid Atlantic salmon ( Salmo salar L.): Description of multiple novel Pax -7 splice variants Gotensparre, S M Anderssen, E Wargelius, A Hansen, T Johnston, Ian Alistair 2006-05-24 https://research-portal.st-andrews.ac.uk/en/researchoutput/insight-into-the-complex-genetic-network-of-tetraploid-atlantic-salmon-salmo-salar-l-description-of-multiple-novel-pax-7-splice-variants(7ddb9309-333c-4070-bc7c-d494e226b0e8).html https://doi.org/10.1016/j.gene.2005.12.026 http://www.scopus.com/inward/record.url?scp=33646271823&partnerID=8YFLogxK eng eng https://research-portal.st-andrews.ac.uk/en/researchoutput/insight-into-the-complex-genetic-network-of-tetraploid-atlantic-salmon-salmo-salar-l-description-of-multiple-novel-pax-7-splice-variants(7ddb9309-333c-4070-bc7c-d494e226b0e8).html info:eu-repo/semantics/restrictedAccess Gotensparre , S M , Anderssen , E , Wargelius , A , Hansen , T & Johnston , I A 2006 , ' Insight into the complex genetic network of tetraploid Atlantic salmon ( Salmo salar L.): Description of multiple novel Pax -7 splice variants ' , Gene , vol. 373 , pp. 8-15 . https://doi.org/10.1016/j.gene.2005.12.026 paralogs expression studies myogenic progenitor cells (MPCs) genomic copies PROTEIN-KINASE CK2 MUSCLE SATELLITE CELLS ADULT MICE EXPRESSION TRANSCRIPTS EVOLUTION GENOME DATABASE ORIGIN GROWTH article 2006 ftunstandrewcris https://doi.org/10.1016/j.gene.2005.12.026 2024-06-13T00:16:28Z Paired box transcription factor 7 (Pax-7) cDNA was isolated from the skeletal muscle and brain of alevin and adult stages of Atlantic salmon, identifying 10 variants categorised as novel or established insertions (ins) or deletions (del). Two putative Pax-7 paralogs were identified (denoted Pax-7 alpha and Pax-7 beta) on the basis of the length and sequences of intron 3 (218 and 248 bp) and versions of ins1 and ins2. Pax-7 beta contained a threonine variant of ins1 (GQY[T]GPEYVYCGT), and a shortened variant of ins2 (GEAS). Pattern identification revealed the threonine variant of ins1 includes a potential phosphorylation site (casein kinase 11). Thus, the tetraploid Atlantic salmon genome appears to contain at least two putative copies and multiple splice variants of Pax-7. In situ hybridisation localised Pax-7 to mononuclear cells in the fast muscle of adult Atlantic salmon, while quantitative real-time PCR showed Pax-7 alpha to be more highly expressed in brain than in skeletal muscle. (c) 2006 Elsevier B.V. All rights reserved. Article in Journal/Newspaper Atlantic salmon Salmo salar University of St Andrews: Research Portal Gene 373 8 15 |
institution |
Open Polar |
collection |
University of St Andrews: Research Portal |
op_collection_id |
ftunstandrewcris |
language |
English |
topic |
paralogs expression studies myogenic progenitor cells (MPCs) genomic copies PROTEIN-KINASE CK2 MUSCLE SATELLITE CELLS ADULT MICE EXPRESSION TRANSCRIPTS EVOLUTION GENOME DATABASE ORIGIN GROWTH |
spellingShingle |
paralogs expression studies myogenic progenitor cells (MPCs) genomic copies PROTEIN-KINASE CK2 MUSCLE SATELLITE CELLS ADULT MICE EXPRESSION TRANSCRIPTS EVOLUTION GENOME DATABASE ORIGIN GROWTH Gotensparre, S M Anderssen, E Wargelius, A Hansen, T Johnston, Ian Alistair Insight into the complex genetic network of tetraploid Atlantic salmon ( Salmo salar L.): Description of multiple novel Pax -7 splice variants |
topic_facet |
paralogs expression studies myogenic progenitor cells (MPCs) genomic copies PROTEIN-KINASE CK2 MUSCLE SATELLITE CELLS ADULT MICE EXPRESSION TRANSCRIPTS EVOLUTION GENOME DATABASE ORIGIN GROWTH |
description |
Paired box transcription factor 7 (Pax-7) cDNA was isolated from the skeletal muscle and brain of alevin and adult stages of Atlantic salmon, identifying 10 variants categorised as novel or established insertions (ins) or deletions (del). Two putative Pax-7 paralogs were identified (denoted Pax-7 alpha and Pax-7 beta) on the basis of the length and sequences of intron 3 (218 and 248 bp) and versions of ins1 and ins2. Pax-7 beta contained a threonine variant of ins1 (GQY[T]GPEYVYCGT), and a shortened variant of ins2 (GEAS). Pattern identification revealed the threonine variant of ins1 includes a potential phosphorylation site (casein kinase 11). Thus, the tetraploid Atlantic salmon genome appears to contain at least two putative copies and multiple splice variants of Pax-7. In situ hybridisation localised Pax-7 to mononuclear cells in the fast muscle of adult Atlantic salmon, while quantitative real-time PCR showed Pax-7 alpha to be more highly expressed in brain than in skeletal muscle. (c) 2006 Elsevier B.V. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Gotensparre, S M Anderssen, E Wargelius, A Hansen, T Johnston, Ian Alistair |
author_facet |
Gotensparre, S M Anderssen, E Wargelius, A Hansen, T Johnston, Ian Alistair |
author_sort |
Gotensparre, S M |
title |
Insight into the complex genetic network of tetraploid Atlantic salmon ( Salmo salar L.): Description of multiple novel Pax -7 splice variants |
title_short |
Insight into the complex genetic network of tetraploid Atlantic salmon ( Salmo salar L.): Description of multiple novel Pax -7 splice variants |
title_full |
Insight into the complex genetic network of tetraploid Atlantic salmon ( Salmo salar L.): Description of multiple novel Pax -7 splice variants |
title_fullStr |
Insight into the complex genetic network of tetraploid Atlantic salmon ( Salmo salar L.): Description of multiple novel Pax -7 splice variants |
title_full_unstemmed |
Insight into the complex genetic network of tetraploid Atlantic salmon ( Salmo salar L.): Description of multiple novel Pax -7 splice variants |
title_sort |
insight into the complex genetic network of tetraploid atlantic salmon ( salmo salar l.): description of multiple novel pax -7 splice variants |
publishDate |
2006 |
url |
https://research-portal.st-andrews.ac.uk/en/researchoutput/insight-into-the-complex-genetic-network-of-tetraploid-atlantic-salmon-salmo-salar-l-description-of-multiple-novel-pax-7-splice-variants(7ddb9309-333c-4070-bc7c-d494e226b0e8).html https://doi.org/10.1016/j.gene.2005.12.026 http://www.scopus.com/inward/record.url?scp=33646271823&partnerID=8YFLogxK |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Gotensparre , S M , Anderssen , E , Wargelius , A , Hansen , T & Johnston , I A 2006 , ' Insight into the complex genetic network of tetraploid Atlantic salmon ( Salmo salar L.): Description of multiple novel Pax -7 splice variants ' , Gene , vol. 373 , pp. 8-15 . https://doi.org/10.1016/j.gene.2005.12.026 |
op_relation |
https://research-portal.st-andrews.ac.uk/en/researchoutput/insight-into-the-complex-genetic-network-of-tetraploid-atlantic-salmon-salmo-salar-l-description-of-multiple-novel-pax-7-splice-variants(7ddb9309-333c-4070-bc7c-d494e226b0e8).html |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.gene.2005.12.026 |
container_title |
Gene |
container_volume |
373 |
container_start_page |
8 |
op_container_end_page |
15 |
_version_ |
1802642222083997696 |