Not just fat:investigating the proteome of cetacean blubber tissue
Mammalian adipose tissue is increasingly being recognized as an endocrine organ involved in the regulation of a number of metabolic processes and pathways. It responds to signals from different hormone systems and the central nervous system, and expresses a variety of protein factors with important...
Published in: | Conservation Physiology |
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2018
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Online Access: | https://research-portal.st-andrews.ac.uk/en/researchoutput/not-just-fat(71ef3bec-1e45-41ba-b28b-e29dbecf0dc4).html https://doi.org/10.1093/conphys/coy003 https://research-repository.st-andrews.ac.uk/bitstream/10023/12818/1/Kershaw_2018_Not_just_fat_ConservPhysiol_CC.pdf |
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ftunstandrewcris:oai:research-portal.st-andrews.ac.uk:publications/71ef3bec-1e45-41ba-b28b-e29dbecf0dc4 2024-06-23T07:56:12+00:00 Not just fat:investigating the proteome of cetacean blubber tissue Kershaw, Joanna Louise Botting, Catherine Helen Brownlow, Andrew Hall, Ailsa Jane 2018 application/pdf https://research-portal.st-andrews.ac.uk/en/researchoutput/not-just-fat(71ef3bec-1e45-41ba-b28b-e29dbecf0dc4).html https://doi.org/10.1093/conphys/coy003 https://research-repository.st-andrews.ac.uk/bitstream/10023/12818/1/Kershaw_2018_Not_just_fat_ConservPhysiol_CC.pdf eng eng https://research-portal.st-andrews.ac.uk/en/researchoutput/not-just-fat(71ef3bec-1e45-41ba-b28b-e29dbecf0dc4).html info:eu-repo/semantics/openAccess Kershaw , J L , Botting , C H , Brownlow , A & Hall , A J 2018 , ' Not just fat : investigating the proteome of cetacean blubber tissue ' , Conservation Physiology , vol. 6 , no. 1 . https://doi.org/10.1093/conphys/coy003 Adipose tissue Biomarkers Marine mammals Metabolism Proteomics article 2018 ftunstandrewcris https://doi.org/10.1093/conphys/coy003 2024-06-13T00:59:15Z Mammalian adipose tissue is increasingly being recognized as an endocrine organ involved in the regulation of a number of metabolic processes and pathways. It responds to signals from different hormone systems and the central nervous system, and expresses a variety of protein factors with important paracrine and endocrine functions. This study presents a first step towards the systematic analysis of the protein content of cetacean adipose tissue, the blubber, in order to investigate the kinds of proteins present and their relative abundance. Full depth blubber subsamples were collected from dead-stranded harbour porpoises ( Phocoena phocoena ) (n = 21). Three total protein extraction methods were trialled, and the highest total protein yields with the lowest extraction variability were achieved using a RIPA cell lysis and extraction buffer based protocol. Extracted proteins were separated using 1D Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE), and identified using nanoflow Liquid Chromatography Electrospray Ionization in tandem with Mass Spectrometry (nLC-ESI–MS/MS). A range of proteins were identified (n = 295) and classed into eight functional groups, the most abundant of which were involved in cell function and metabolism (45%), immune response and inflammation (15%) and lipid metabolism (11%). These proteins likely originate both from the various cell types within the blubber tissue itself, and from the circulation. They therefore have the potential to capture information on the cellular and physiological stresses experienced by individuals at the time of sampling. The importance of this proteomic approach is two-fold: Firstly, it could help to assign novel functions to marine mammal blubber in keeping with current understanding of the multi-functional role of adipose tissue in other mammals. Secondly, it could lead to the development of a suite of biomarkers to better monitor the physiological state and health of live individuals though remote blubber biopsy sampling. Article in Journal/Newspaper Phocoena phocoena University of St Andrews: Research Portal Conservation Physiology 6 1 |
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Open Polar |
collection |
University of St Andrews: Research Portal |
op_collection_id |
ftunstandrewcris |
language |
English |
topic |
Adipose tissue Biomarkers Marine mammals Metabolism Proteomics |
spellingShingle |
Adipose tissue Biomarkers Marine mammals Metabolism Proteomics Kershaw, Joanna Louise Botting, Catherine Helen Brownlow, Andrew Hall, Ailsa Jane Not just fat:investigating the proteome of cetacean blubber tissue |
topic_facet |
Adipose tissue Biomarkers Marine mammals Metabolism Proteomics |
description |
Mammalian adipose tissue is increasingly being recognized as an endocrine organ involved in the regulation of a number of metabolic processes and pathways. It responds to signals from different hormone systems and the central nervous system, and expresses a variety of protein factors with important paracrine and endocrine functions. This study presents a first step towards the systematic analysis of the protein content of cetacean adipose tissue, the blubber, in order to investigate the kinds of proteins present and their relative abundance. Full depth blubber subsamples were collected from dead-stranded harbour porpoises ( Phocoena phocoena ) (n = 21). Three total protein extraction methods were trialled, and the highest total protein yields with the lowest extraction variability were achieved using a RIPA cell lysis and extraction buffer based protocol. Extracted proteins were separated using 1D Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE), and identified using nanoflow Liquid Chromatography Electrospray Ionization in tandem with Mass Spectrometry (nLC-ESI–MS/MS). A range of proteins were identified (n = 295) and classed into eight functional groups, the most abundant of which were involved in cell function and metabolism (45%), immune response and inflammation (15%) and lipid metabolism (11%). These proteins likely originate both from the various cell types within the blubber tissue itself, and from the circulation. They therefore have the potential to capture information on the cellular and physiological stresses experienced by individuals at the time of sampling. The importance of this proteomic approach is two-fold: Firstly, it could help to assign novel functions to marine mammal blubber in keeping with current understanding of the multi-functional role of adipose tissue in other mammals. Secondly, it could lead to the development of a suite of biomarkers to better monitor the physiological state and health of live individuals though remote blubber biopsy sampling. |
format |
Article in Journal/Newspaper |
author |
Kershaw, Joanna Louise Botting, Catherine Helen Brownlow, Andrew Hall, Ailsa Jane |
author_facet |
Kershaw, Joanna Louise Botting, Catherine Helen Brownlow, Andrew Hall, Ailsa Jane |
author_sort |
Kershaw, Joanna Louise |
title |
Not just fat:investigating the proteome of cetacean blubber tissue |
title_short |
Not just fat:investigating the proteome of cetacean blubber tissue |
title_full |
Not just fat:investigating the proteome of cetacean blubber tissue |
title_fullStr |
Not just fat:investigating the proteome of cetacean blubber tissue |
title_full_unstemmed |
Not just fat:investigating the proteome of cetacean blubber tissue |
title_sort |
not just fat:investigating the proteome of cetacean blubber tissue |
publishDate |
2018 |
url |
https://research-portal.st-andrews.ac.uk/en/researchoutput/not-just-fat(71ef3bec-1e45-41ba-b28b-e29dbecf0dc4).html https://doi.org/10.1093/conphys/coy003 https://research-repository.st-andrews.ac.uk/bitstream/10023/12818/1/Kershaw_2018_Not_just_fat_ConservPhysiol_CC.pdf |
genre |
Phocoena phocoena |
genre_facet |
Phocoena phocoena |
op_source |
Kershaw , J L , Botting , C H , Brownlow , A & Hall , A J 2018 , ' Not just fat : investigating the proteome of cetacean blubber tissue ' , Conservation Physiology , vol. 6 , no. 1 . https://doi.org/10.1093/conphys/coy003 |
op_relation |
https://research-portal.st-andrews.ac.uk/en/researchoutput/not-just-fat(71ef3bec-1e45-41ba-b28b-e29dbecf0dc4).html |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1093/conphys/coy003 |
container_title |
Conservation Physiology |
container_volume |
6 |
container_issue |
1 |
_version_ |
1802649144864538624 |