Myogenic cell cycle duration in Harpagifer species with Sub-Antarctic and Antarctic distributions: evidence for cold compensation

In teleosts, the proliferation of myogenic progenitor cells is required for muscle growth and nuclear turnover. We measured the cell cycle and S-phase duration of myogenic cells in the fast myotomal muscle of two closely related Harpagifer species by cumulative S-phase labelling with 5-bromo-2'...

Full description

Bibliographic Details
Published in:Journal of Experimental Biology
Main Authors: Brodeur, JC, Calvo, J, Clarke, A, Johnston, Ian Alistair
Format: Article in Journal/Newspaper
Language:English
Published: 2003
Subjects:
cell cycle
S-phase
Harpagifer bispinis
Harpagifer antarcticus
myogenic progenitor cell
cold compensation
Antarctic
temperature
notothenioid fish
satellite cell
NOTOTHENIA CORIICEPS RICHARDSON
MUSCLE POWER OUTPUT
SKELETAL-MUSCLE
TELEOST FISH
GROWTH
ADAPTATION
EVOLUTION
FIBERS
Antarctic Peninsula
The Antarctic
Antarc*
antarcticus
Tierra del Fuego
Online Access:https://research-portal.st-andrews.ac.uk/en/researchoutput/myogenic-cell-cycle-duration-in-harpagifer-species-with-subantarctic-and-antarctic-distributions-evidence-for-cold-compensation(5cbd5862-99b0-40b6-a32e-77b7de1188b9).html
https://doi.org/10.1242/jeb.00204
http://www.scopus.com/inward/record.url?scp=0242417459&partnerID=8YFLogxK
Description
Summary:In teleosts, the proliferation of myogenic progenitor cells is required for muscle growth and nuclear turnover. We measured the cell cycle and S-phase duration of myogenic cells in the fast myotomal muscle of two closely related Harpagifer species by cumulative S-phase labelling with 5-bromo-2'-deoxyuridine (BrdU). Harpagifer antarcticus is a stenothermal species from the Antarctic peninsula (experiencing temperatures of -2degreesC to +1degreesC) and Harpagifer bispinis is a eurythermal species from the Beagle Channel, Tierra del Fuego (living at +4degreesC in winter and up to 11degreesC in summer). Specific growth rates in the adult stages studied were not significantly different from zero. Myogenic progenitor cells were identified using an antibody against c-met. Seventy-five percent of the c-met(+ve) cells were in a proliferative state in both species. Cell cycle time was 150h at 5degreesC and 81.3h at 10degreesC in H. bispinis (Q(10)=3.4). Cell cycle duration was 35% shorter in H. antarcticus at 0degreesC (111h) than in H. bispinis at 5degreesC. The predicted cell cycle time for H. bispinis at 0degreesC (based on the Q(10) relationship) was 277h, which was more than double that measured for the Antarctic species at this temperature. The results obtained are compatible with an evolutionary adjustment of cell cycle time for function at low temperature in the Antarctic species.

Similar Items