Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques
The accurate observation of physiological changes on in vivo samples of important animal species such as Euphasia superba (Antarctic krill) is an important goal in helping to understand how environmental changes can affect animal development. Using a custom made ‘krill trap’, live un-anaesthetized k...
Published in: | Marine and Freshwater Behaviour and Physiology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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2015
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Online Access: | https://research-portal.st-andrews.ac.uk/en/researchoutput/internal-physiology-of-live-krill-revealed-using-new-aquaria-techniques-and-mixed-optical-microscopy-and-optical-coherence-tomography-oct-imaging-techniques(4701e3fb-2832-4f5c-94c7-d5cb70e3eea5).html https://doi.org/10.1080/10236244.2015.1073455 https://research-repository.st-andrews.ac.uk/bitstream/10023/9376/1/MFBP2015_1_5_preprint.pdf http://www.tandfonline.com/doi/full/10.1080/10236244.2015.1073455#_i19 |
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ftunstandrewcris:oai:research-portal.st-andrews.ac.uk:publications/4701e3fb-2832-4f5c-94c7-d5cb70e3eea5 2024-06-23T07:47:27+00:00 Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques Cox, Martin Kawaguchu, So King, Robert Dholakia, Kishan Brown, C Tom A 2015 application/pdf https://research-portal.st-andrews.ac.uk/en/researchoutput/internal-physiology-of-live-krill-revealed-using-new-aquaria-techniques-and-mixed-optical-microscopy-and-optical-coherence-tomography-oct-imaging-techniques(4701e3fb-2832-4f5c-94c7-d5cb70e3eea5).html https://doi.org/10.1080/10236244.2015.1073455 https://research-repository.st-andrews.ac.uk/bitstream/10023/9376/1/MFBP2015_1_5_preprint.pdf http://www.tandfonline.com/doi/full/10.1080/10236244.2015.1073455#_i19 eng eng https://research-portal.st-andrews.ac.uk/en/researchoutput/internal-physiology-of-live-krill-revealed-using-new-aquaria-techniques-and-mixed-optical-microscopy-and-optical-coherence-tomography-oct-imaging-techniques(4701e3fb-2832-4f5c-94c7-d5cb70e3eea5).html info:eu-repo/semantics/openAccess Cox , M , Kawaguchu , S , King , R , Dholakia , K & Brown , C T A 2015 , ' Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques ' , Marine and Freshwater Behaviour and Physiology , vol. In press . https://doi.org/10.1080/10236244.2015.1073455 Krill trap Live animal Morphological observations Optical Coherence Tomography Optical imaging article 2015 ftunstandrewcris https://doi.org/10.1080/10236244.2015.1073455 2024-06-13T00:48:07Z The accurate observation of physiological changes on in vivo samples of important animal species such as Euphasia superba (Antarctic krill) is an important goal in helping to understand how environmental changes can affect animal development. Using a custom made ‘krill trap’, live un-anaesthetized krill were confined for seven hours, during which three hours of optical imaging were obtained and no subsequent ill effects observed. The trap enabled two imaging methods to be employed: Optical Coherence Tomography (OCT) and microscopy. OCT enabled internal structure and tissues to be imaged to a depth of approximately 2 mm and resolution of approximately 12 μm. Microscopy was used to observe heart rate. During our experiments, we imaged a range of internal structures in live animals including the heart and gastric areas. The trap design enables a new generation of mixed modality imaging of these animals in vivo. These techniques will enable detailed studies of the internal physiology of live krill to be undertaken under a wide range of environmental conditions and have the potential to highlight important variations in behaviour and animal development. Article in Journal/Newspaper Antarc* Antarctic Antarctic Krill University of St Andrews: Research Portal Antarctic Marine and Freshwater Behaviour and Physiology 48 6 455 466 |
institution |
Open Polar |
collection |
University of St Andrews: Research Portal |
op_collection_id |
ftunstandrewcris |
language |
English |
topic |
Krill trap Live animal Morphological observations Optical Coherence Tomography Optical imaging |
spellingShingle |
Krill trap Live animal Morphological observations Optical Coherence Tomography Optical imaging Cox, Martin Kawaguchu, So King, Robert Dholakia, Kishan Brown, C Tom A Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques |
topic_facet |
Krill trap Live animal Morphological observations Optical Coherence Tomography Optical imaging |
description |
The accurate observation of physiological changes on in vivo samples of important animal species such as Euphasia superba (Antarctic krill) is an important goal in helping to understand how environmental changes can affect animal development. Using a custom made ‘krill trap’, live un-anaesthetized krill were confined for seven hours, during which three hours of optical imaging were obtained and no subsequent ill effects observed. The trap enabled two imaging methods to be employed: Optical Coherence Tomography (OCT) and microscopy. OCT enabled internal structure and tissues to be imaged to a depth of approximately 2 mm and resolution of approximately 12 μm. Microscopy was used to observe heart rate. During our experiments, we imaged a range of internal structures in live animals including the heart and gastric areas. The trap design enables a new generation of mixed modality imaging of these animals in vivo. These techniques will enable detailed studies of the internal physiology of live krill to be undertaken under a wide range of environmental conditions and have the potential to highlight important variations in behaviour and animal development. |
format |
Article in Journal/Newspaper |
author |
Cox, Martin Kawaguchu, So King, Robert Dholakia, Kishan Brown, C Tom A |
author_facet |
Cox, Martin Kawaguchu, So King, Robert Dholakia, Kishan Brown, C Tom A |
author_sort |
Cox, Martin |
title |
Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques |
title_short |
Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques |
title_full |
Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques |
title_fullStr |
Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques |
title_full_unstemmed |
Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques |
title_sort |
internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (oct) imaging techniques |
publishDate |
2015 |
url |
https://research-portal.st-andrews.ac.uk/en/researchoutput/internal-physiology-of-live-krill-revealed-using-new-aquaria-techniques-and-mixed-optical-microscopy-and-optical-coherence-tomography-oct-imaging-techniques(4701e3fb-2832-4f5c-94c7-d5cb70e3eea5).html https://doi.org/10.1080/10236244.2015.1073455 https://research-repository.st-andrews.ac.uk/bitstream/10023/9376/1/MFBP2015_1_5_preprint.pdf http://www.tandfonline.com/doi/full/10.1080/10236244.2015.1073455#_i19 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctic Krill |
genre_facet |
Antarc* Antarctic Antarctic Krill |
op_source |
Cox , M , Kawaguchu , S , King , R , Dholakia , K & Brown , C T A 2015 , ' Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques ' , Marine and Freshwater Behaviour and Physiology , vol. In press . https://doi.org/10.1080/10236244.2015.1073455 |
op_relation |
https://research-portal.st-andrews.ac.uk/en/researchoutput/internal-physiology-of-live-krill-revealed-using-new-aquaria-techniques-and-mixed-optical-microscopy-and-optical-coherence-tomography-oct-imaging-techniques(4701e3fb-2832-4f5c-94c7-d5cb70e3eea5).html |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1080/10236244.2015.1073455 |
container_title |
Marine and Freshwater Behaviour and Physiology |
container_volume |
48 |
container_issue |
6 |
container_start_page |
455 |
op_container_end_page |
466 |
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1802651561420128256 |