Phylogeography of the Arctic-Alpine Saxifraga oppositifolia (Saxifragaceae) and some related taxa based on cpDNA and its sequence variation
Saxifraga oppositifolia (Saxifragaceae) is an important model system for the evolution of Arctic-Alpine plant species. Sequences of the psbA-trnH intergenic spacer of chloroplast DNA and of the internal transcribed spacer region, ITS1-5.8S-ITS2, of the nuclear ribosomal DNA were used to investigate...
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2003
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Online Access: | https://research-portal.st-andrews.ac.uk/en/researchoutput/phylogeography-of-the-arcticalpine-saxifraga-oppositifolia-saxifragaceae-and-some-related-taxa-based-on-cpdna-and-its-sequence-variation(40183d37-144e-4655-ade7-84d960fefe5b).html http://www.scopus.com/inward/record.url?scp=0038348300&partnerID=8YFLogxK |
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ftunstandrewcris:oai:research-portal.st-andrews.ac.uk:publications/40183d37-144e-4655-ade7-84d960fefe5b 2024-06-23T07:48:39+00:00 Phylogeography of the Arctic-Alpine Saxifraga oppositifolia (Saxifragaceae) and some related taxa based on cpDNA and its sequence variation Holderegger, R Abbott, R J 2003-06 https://research-portal.st-andrews.ac.uk/en/researchoutput/phylogeography-of-the-arcticalpine-saxifraga-oppositifolia-saxifragaceae-and-some-related-taxa-based-on-cpdna-and-its-sequence-variation(40183d37-144e-4655-ade7-84d960fefe5b).html http://www.scopus.com/inward/record.url?scp=0038348300&partnerID=8YFLogxK eng eng https://research-portal.st-andrews.ac.uk/en/researchoutput/phylogeography-of-the-arcticalpine-saxifraga-oppositifolia-saxifragaceae-and-some-related-taxa-based-on-cpdna-and-its-sequence-variation(40183d37-144e-4655-ade7-84d960fefe5b).html info:eu-repo/semantics/restrictedAccess Holderegger , R & Abbott , R J 2003 , ' Phylogeography of the Arctic-Alpine Saxifraga oppositifolia (Saxifragaceae) and some related taxa based on cpDNA and its sequence variation ' , American Journal of Botany , vol. 90 , pp. 931-936 . contact zones intraspecific evolution ITS lineage sorting long-distance dispersal phylogeography psbA-trnH cpDNA Saxifraga oppositifolia Saxifragaceae NUCLEAR RIBOSOMAL DNA PHYLOGENETIC-RELATIONSHIPS RETICULATE EVOLUTION MOLECULAR EVIDENCE POPULATIONS BIOGEOGRAPHY HISTORY RAPD HYBRIDIZATION DISCORDANCE article 2003 ftunstandrewcris 2024-06-13T00:26:44Z Saxifraga oppositifolia (Saxifragaceae) is an important model system for the evolution of Arctic-Alpine plant species. Sequences of the psbA-trnH intergenic spacer of chloroplast DNA and of the internal transcribed spacer region, ITS1-5.8S-ITS2, of the nuclear ribosomal DNA were used to investigate the intraspecific evolution and phylogeography of this species. Samples from nearly the species' entire circumpolar distribution were included in the analysis as well as samples from the closely related taxa S. smalliana from Alaska and S. blepharophylla and S. biflora from the Alps (S. aizoides served as outgroup). These latter taxa showed a low number of parsimony informative characters, in both cpDNA and ITS sequences, which separated them from S. oppositifolia. Two main cpDNA haplotypes were detected within S. oppositifolia, one with a Eurasian distribution and one with an East Asian-North American distribution. This confirmed the existence of two cpDNA lineages with different geographical distributions in this species, which had previously been reported based on a RFLP analysis. The ITS phylogeny was not useful with respect to the intraspecific evolution and phylogeography of S. oppositifolia, because it showed a largely unresolved topology with low statistical support. The cpDNA sequence analysis, however, also suggested a putative long-distance dispersal event. All investigated taxa had cpDNA haplotypes that were congruent with their geographical origin rather than their phylogeny. This could point to putative lineage sorting in S. oppositifolia and related taxa. Article in Journal/Newspaper Arctic Arctic Saxifraga oppositifolia Alaska University of St Andrews: Research Portal Arctic |
institution |
Open Polar |
collection |
University of St Andrews: Research Portal |
op_collection_id |
ftunstandrewcris |
language |
English |
topic |
contact zones intraspecific evolution ITS lineage sorting long-distance dispersal phylogeography psbA-trnH cpDNA Saxifraga oppositifolia Saxifragaceae NUCLEAR RIBOSOMAL DNA PHYLOGENETIC-RELATIONSHIPS RETICULATE EVOLUTION MOLECULAR EVIDENCE POPULATIONS BIOGEOGRAPHY HISTORY RAPD HYBRIDIZATION DISCORDANCE |
spellingShingle |
contact zones intraspecific evolution ITS lineage sorting long-distance dispersal phylogeography psbA-trnH cpDNA Saxifraga oppositifolia Saxifragaceae NUCLEAR RIBOSOMAL DNA PHYLOGENETIC-RELATIONSHIPS RETICULATE EVOLUTION MOLECULAR EVIDENCE POPULATIONS BIOGEOGRAPHY HISTORY RAPD HYBRIDIZATION DISCORDANCE Holderegger, R Abbott, R J Phylogeography of the Arctic-Alpine Saxifraga oppositifolia (Saxifragaceae) and some related taxa based on cpDNA and its sequence variation |
topic_facet |
contact zones intraspecific evolution ITS lineage sorting long-distance dispersal phylogeography psbA-trnH cpDNA Saxifraga oppositifolia Saxifragaceae NUCLEAR RIBOSOMAL DNA PHYLOGENETIC-RELATIONSHIPS RETICULATE EVOLUTION MOLECULAR EVIDENCE POPULATIONS BIOGEOGRAPHY HISTORY RAPD HYBRIDIZATION DISCORDANCE |
description |
Saxifraga oppositifolia (Saxifragaceae) is an important model system for the evolution of Arctic-Alpine plant species. Sequences of the psbA-trnH intergenic spacer of chloroplast DNA and of the internal transcribed spacer region, ITS1-5.8S-ITS2, of the nuclear ribosomal DNA were used to investigate the intraspecific evolution and phylogeography of this species. Samples from nearly the species' entire circumpolar distribution were included in the analysis as well as samples from the closely related taxa S. smalliana from Alaska and S. blepharophylla and S. biflora from the Alps (S. aizoides served as outgroup). These latter taxa showed a low number of parsimony informative characters, in both cpDNA and ITS sequences, which separated them from S. oppositifolia. Two main cpDNA haplotypes were detected within S. oppositifolia, one with a Eurasian distribution and one with an East Asian-North American distribution. This confirmed the existence of two cpDNA lineages with different geographical distributions in this species, which had previously been reported based on a RFLP analysis. The ITS phylogeny was not useful with respect to the intraspecific evolution and phylogeography of S. oppositifolia, because it showed a largely unresolved topology with low statistical support. The cpDNA sequence analysis, however, also suggested a putative long-distance dispersal event. All investigated taxa had cpDNA haplotypes that were congruent with their geographical origin rather than their phylogeny. This could point to putative lineage sorting in S. oppositifolia and related taxa. |
format |
Article in Journal/Newspaper |
author |
Holderegger, R Abbott, R J |
author_facet |
Holderegger, R Abbott, R J |
author_sort |
Holderegger, R |
title |
Phylogeography of the Arctic-Alpine Saxifraga oppositifolia (Saxifragaceae) and some related taxa based on cpDNA and its sequence variation |
title_short |
Phylogeography of the Arctic-Alpine Saxifraga oppositifolia (Saxifragaceae) and some related taxa based on cpDNA and its sequence variation |
title_full |
Phylogeography of the Arctic-Alpine Saxifraga oppositifolia (Saxifragaceae) and some related taxa based on cpDNA and its sequence variation |
title_fullStr |
Phylogeography of the Arctic-Alpine Saxifraga oppositifolia (Saxifragaceae) and some related taxa based on cpDNA and its sequence variation |
title_full_unstemmed |
Phylogeography of the Arctic-Alpine Saxifraga oppositifolia (Saxifragaceae) and some related taxa based on cpDNA and its sequence variation |
title_sort |
phylogeography of the arctic-alpine saxifraga oppositifolia (saxifragaceae) and some related taxa based on cpdna and its sequence variation |
publishDate |
2003 |
url |
https://research-portal.st-andrews.ac.uk/en/researchoutput/phylogeography-of-the-arcticalpine-saxifraga-oppositifolia-saxifragaceae-and-some-related-taxa-based-on-cpdna-and-its-sequence-variation(40183d37-144e-4655-ade7-84d960fefe5b).html http://www.scopus.com/inward/record.url?scp=0038348300&partnerID=8YFLogxK |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Arctic Saxifraga oppositifolia Alaska |
genre_facet |
Arctic Arctic Saxifraga oppositifolia Alaska |
op_source |
Holderegger , R & Abbott , R J 2003 , ' Phylogeography of the Arctic-Alpine Saxifraga oppositifolia (Saxifragaceae) and some related taxa based on cpDNA and its sequence variation ' , American Journal of Botany , vol. 90 , pp. 931-936 . |
op_relation |
https://research-portal.st-andrews.ac.uk/en/researchoutput/phylogeography-of-the-arcticalpine-saxifraga-oppositifolia-saxifragaceae-and-some-related-taxa-based-on-cpdna-and-its-sequence-variation(40183d37-144e-4655-ade7-84d960fefe5b).html |
op_rights |
info:eu-repo/semantics/restrictedAccess |
_version_ |
1802638994283954176 |