In vitro cytotoxicity of crustacean immunostimulants for lobster haemocytes using the neutral red uptake assay.
The neutral red uptake (NRU) cell viability assay was adapted for use with lobster Homarus gammarus (Linnaeus, 1758) granulocytes Cultured in vitro. The assay was more sensitive than the conventional trypan blue exclusion assay and facilitated a higher sample throughput than subjective microscope-ba...
Published in: | Fish & Shellfish Immunology |
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Language: | English |
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2004
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Online Access: | https://research-portal.st-andrews.ac.uk/en/researchoutput/in-vitro-cytotoxicity-of-crustacean-immunostimulants-for-lobster-haemocytes-using-the-neutral-red-uptake-assay(1db31609-62b9-4e65-b3c0-a524b77b1758).html https://doi.org/10.1016/j.fsi.2003.12.003 http://www.scopus.com/inward/record.url?scp=3042693346&partnerID=8YFLogxK |
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ftunstandrewcris:oai:research-portal.st-andrews.ac.uk:publications/1db31609-62b9-4e65-b3c0-a524b77b1758 2024-06-23T07:53:33+00:00 In vitro cytotoxicity of crustacean immunostimulants for lobster haemocytes using the neutral red uptake assay. Hauton, Christopher Smith, Valerie Jane 2004-07 https://research-portal.st-andrews.ac.uk/en/researchoutput/in-vitro-cytotoxicity-of-crustacean-immunostimulants-for-lobster-haemocytes-using-the-neutral-red-uptake-assay(1db31609-62b9-4e65-b3c0-a524b77b1758).html https://doi.org/10.1016/j.fsi.2003.12.003 http://www.scopus.com/inward/record.url?scp=3042693346&partnerID=8YFLogxK eng eng https://research-portal.st-andrews.ac.uk/en/researchoutput/in-vitro-cytotoxicity-of-crustacean-immunostimulants-for-lobster-haemocytes-using-the-neutral-red-uptake-assay(1db31609-62b9-4e65-b3c0-a524b77b1758).html info:eu-repo/semantics/restrictedAccess Hauton , C & Smith , V J 2004 , In vitro cytotoxicity of crustacean immunostimulants for lobster haemocytes using the neutral red uptake assay. . https://doi.org/10.1016/j.fsi.2003.12.003 aquaculture crustaceans Homarus gammarus immunostimulation neutral red uptake assay CARCINUS-MAENAS L VIBRIOSIS RESISTANCE LITOPENAEUS-VANNAMEI PENAEUS-MONODON MARINE DECAPODS SHORE CRAB INVITRO IMMUNOSTIMULANTS PROPHENOLOXIDASE VARIABILITY other 2004 ftunstandrewcris https://doi.org/10.1016/j.fsi.2003.12.003 2024-06-13T00:14:39Z The neutral red uptake (NRU) cell viability assay was adapted for use with lobster Homarus gammarus (Linnaeus, 1758) granulocytes Cultured in vitro. The assay was more sensitive than the conventional trypan blue exclusion assay and facilitated a higher sample throughput than subjective microscope-based assessments of cell viability. The NRU assay was demonstrated to have a linear response from 470 to at least 126 000 cells cm(-2). It was used to investigate the acute cytotoxicity of three commercial and two candidate crustacean aquaculture immunostimulants on lobster granulocytes. All five stimulants had a cytotoxic action on the granulocytes and the toxic dose for some of these stimulants was found to be below their commercially prescribed dose. The long term energetic cost of the use of these stimulants and the concomitant potential for a reduction in growth rate Of Cultured decapod crustaceans, which is fundamental to the Success of commercial aquaculture, is identified and discussed. (C) 2004 Elsevier Ltd. All rights reserved. Other/Unknown Material Homarus gammarus University of St Andrews: Research Portal Fish & Shellfish Immunology 17 1 65 73 |
institution |
Open Polar |
collection |
University of St Andrews: Research Portal |
op_collection_id |
ftunstandrewcris |
language |
English |
topic |
aquaculture crustaceans Homarus gammarus immunostimulation neutral red uptake assay CARCINUS-MAENAS L VIBRIOSIS RESISTANCE LITOPENAEUS-VANNAMEI PENAEUS-MONODON MARINE DECAPODS SHORE CRAB INVITRO IMMUNOSTIMULANTS PROPHENOLOXIDASE VARIABILITY |
spellingShingle |
aquaculture crustaceans Homarus gammarus immunostimulation neutral red uptake assay CARCINUS-MAENAS L VIBRIOSIS RESISTANCE LITOPENAEUS-VANNAMEI PENAEUS-MONODON MARINE DECAPODS SHORE CRAB INVITRO IMMUNOSTIMULANTS PROPHENOLOXIDASE VARIABILITY Hauton, Christopher Smith, Valerie Jane In vitro cytotoxicity of crustacean immunostimulants for lobster haemocytes using the neutral red uptake assay. |
topic_facet |
aquaculture crustaceans Homarus gammarus immunostimulation neutral red uptake assay CARCINUS-MAENAS L VIBRIOSIS RESISTANCE LITOPENAEUS-VANNAMEI PENAEUS-MONODON MARINE DECAPODS SHORE CRAB INVITRO IMMUNOSTIMULANTS PROPHENOLOXIDASE VARIABILITY |
description |
The neutral red uptake (NRU) cell viability assay was adapted for use with lobster Homarus gammarus (Linnaeus, 1758) granulocytes Cultured in vitro. The assay was more sensitive than the conventional trypan blue exclusion assay and facilitated a higher sample throughput than subjective microscope-based assessments of cell viability. The NRU assay was demonstrated to have a linear response from 470 to at least 126 000 cells cm(-2). It was used to investigate the acute cytotoxicity of three commercial and two candidate crustacean aquaculture immunostimulants on lobster granulocytes. All five stimulants had a cytotoxic action on the granulocytes and the toxic dose for some of these stimulants was found to be below their commercially prescribed dose. The long term energetic cost of the use of these stimulants and the concomitant potential for a reduction in growth rate Of Cultured decapod crustaceans, which is fundamental to the Success of commercial aquaculture, is identified and discussed. (C) 2004 Elsevier Ltd. All rights reserved. |
format |
Other/Unknown Material |
author |
Hauton, Christopher Smith, Valerie Jane |
author_facet |
Hauton, Christopher Smith, Valerie Jane |
author_sort |
Hauton, Christopher |
title |
In vitro cytotoxicity of crustacean immunostimulants for lobster haemocytes using the neutral red uptake assay. |
title_short |
In vitro cytotoxicity of crustacean immunostimulants for lobster haemocytes using the neutral red uptake assay. |
title_full |
In vitro cytotoxicity of crustacean immunostimulants for lobster haemocytes using the neutral red uptake assay. |
title_fullStr |
In vitro cytotoxicity of crustacean immunostimulants for lobster haemocytes using the neutral red uptake assay. |
title_full_unstemmed |
In vitro cytotoxicity of crustacean immunostimulants for lobster haemocytes using the neutral red uptake assay. |
title_sort |
in vitro cytotoxicity of crustacean immunostimulants for lobster haemocytes using the neutral red uptake assay. |
publishDate |
2004 |
url |
https://research-portal.st-andrews.ac.uk/en/researchoutput/in-vitro-cytotoxicity-of-crustacean-immunostimulants-for-lobster-haemocytes-using-the-neutral-red-uptake-assay(1db31609-62b9-4e65-b3c0-a524b77b1758).html https://doi.org/10.1016/j.fsi.2003.12.003 http://www.scopus.com/inward/record.url?scp=3042693346&partnerID=8YFLogxK |
genre |
Homarus gammarus |
genre_facet |
Homarus gammarus |
op_source |
Hauton , C & Smith , V J 2004 , In vitro cytotoxicity of crustacean immunostimulants for lobster haemocytes using the neutral red uptake assay. . https://doi.org/10.1016/j.fsi.2003.12.003 |
op_relation |
https://research-portal.st-andrews.ac.uk/en/researchoutput/in-vitro-cytotoxicity-of-crustacean-immunostimulants-for-lobster-haemocytes-using-the-neutral-red-uptake-assay(1db31609-62b9-4e65-b3c0-a524b77b1758).html |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.fsi.2003.12.003 |
container_title |
Fish & Shellfish Immunology |
container_volume |
17 |
container_issue |
1 |
container_start_page |
65 |
op_container_end_page |
73 |
_version_ |
1802645266875023360 |