Phasing of muscle gene expression with fasting-induced recovery growth in Atlantic salmon
Background: Many fish species experience long periods of fasting in nature often associated with seasonal reductions in water temperature and prey availability or spawning migrations. During periods of nutrient restriction, changes in metabolism occur to provide cellular energy via catabolic process...
Published in: | Frontiers in Zoology |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2009
|
Subjects: | |
Online Access: | https://research-portal.st-andrews.ac.uk/en/researchoutput/phasing-of-muscle-gene-expression-with-fastinginduced-recovery-growth-in-atlantic-salmon(199319d1-163f-4ab9-9400-cd4b70fafb49).html https://doi.org/10.1186/1742-9994-6-18 https://research-repository.st-andrews.ac.uk/bitstream/10023/3328/1/Bower2009FrontiersZoology1742_9994_6_18.pdf http://www.scopus.com/inward/record.url?scp=69949106349&partnerID=8YFLogxK |
id |
ftunstandrewcris:oai:research-portal.st-andrews.ac.uk:publications/199319d1-163f-4ab9-9400-cd4b70fafb49 |
---|---|
record_format |
openpolar |
spelling |
ftunstandrewcris:oai:research-portal.st-andrews.ac.uk:publications/199319d1-163f-4ab9-9400-cd4b70fafb49 2024-06-23T07:51:21+00:00 Phasing of muscle gene expression with fasting-induced recovery growth in Atlantic salmon Bower, Neil I. Taylor, Richard G. Johnston, Ian A. 2009-08-24 application/pdf https://research-portal.st-andrews.ac.uk/en/researchoutput/phasing-of-muscle-gene-expression-with-fastinginduced-recovery-growth-in-atlantic-salmon(199319d1-163f-4ab9-9400-cd4b70fafb49).html https://doi.org/10.1186/1742-9994-6-18 https://research-repository.st-andrews.ac.uk/bitstream/10023/3328/1/Bower2009FrontiersZoology1742_9994_6_18.pdf http://www.scopus.com/inward/record.url?scp=69949106349&partnerID=8YFLogxK eng eng https://research-portal.st-andrews.ac.uk/en/researchoutput/phasing-of-muscle-gene-expression-with-fastinginduced-recovery-growth-in-atlantic-salmon(199319d1-163f-4ab9-9400-cd4b70fafb49).html info:eu-repo/semantics/openAccess Bower , N I , Taylor , R G & Johnston , I A 2009 , ' Phasing of muscle gene expression with fasting-induced recovery growth in Atlantic salmon ' , Frontiers in Zoology , vol. 6 , 18 . https://doi.org/10.1186/1742-9994-6-18 Trout oncorhynchus-mykiss Skeletal-muscle Rainbow-trout Juvenile salmon Salar L Regulatory factors Energy-metabolism Ubiquitin-ligase Protein-synthesis Enzyme-activities article 2009 ftunstandrewcris https://doi.org/10.1186/1742-9994-6-18 2024-06-13T00:28:13Z Background: Many fish species experience long periods of fasting in nature often associated with seasonal reductions in water temperature and prey availability or spawning migrations. During periods of nutrient restriction, changes in metabolism occur to provide cellular energy via catabolic processes. Muscle is particularly affected by prolonged fasting as myofibrillar proteins act as a major energy source. To investigate the mechanisms of metabolic reorganisation with fasting and refeeding in a saltwater stage of Atlantic salmon (Salmo salar L.) we analysed the expression of genes involved in myogenesis, growth signalling, lipid biosynthesis and myofibrillar protein degradation and synthesis pathways using qPCR. Results: Hierarchical clustering of gene expression data revealed three clusters. The first cluster comprised genes involved in lipid metabolism and triacylglycerol synthesis (ALDOB, DGAT1 and LPL) which had peak expression 3-14d after refeeding. The second cluster comprised ADIPOQ, MLC2, IGF-I and TALDO1, with peak expression 14-32d after refeeding. Cluster III contained genes strongly down regulated as an initial response to feeding and included the ubiquitin ligases MuRF1 and MAFbx, myogenic regulatory factors and some metabolic genes. Conclusion: Early responses to refeeding in fasted salmon included the synthesis of triacylglycerols and activation of the adipogenic differentiation program. Inhibition of MuRF1 and MAFbx respectively may result in decreased degradation and concomitant increased production of myofibrillar proteins. Both of these processes preceded any increase in expression of myogenic regulatory factors and IGF-I. These responses could be a necessary strategy for an animal adapted to long periods of food deprivation whereby energy reserves are replenished prior to the resumption of myogenesis. Article in Journal/Newspaper Atlantic salmon Salmo salar University of St Andrews: Research Portal Frontiers in Zoology 6 1 |
institution |
Open Polar |
collection |
University of St Andrews: Research Portal |
op_collection_id |
ftunstandrewcris |
language |
English |
topic |
Trout oncorhynchus-mykiss Skeletal-muscle Rainbow-trout Juvenile salmon Salar L Regulatory factors Energy-metabolism Ubiquitin-ligase Protein-synthesis Enzyme-activities |
spellingShingle |
Trout oncorhynchus-mykiss Skeletal-muscle Rainbow-trout Juvenile salmon Salar L Regulatory factors Energy-metabolism Ubiquitin-ligase Protein-synthesis Enzyme-activities Bower, Neil I. Taylor, Richard G. Johnston, Ian A. Phasing of muscle gene expression with fasting-induced recovery growth in Atlantic salmon |
topic_facet |
Trout oncorhynchus-mykiss Skeletal-muscle Rainbow-trout Juvenile salmon Salar L Regulatory factors Energy-metabolism Ubiquitin-ligase Protein-synthesis Enzyme-activities |
description |
Background: Many fish species experience long periods of fasting in nature often associated with seasonal reductions in water temperature and prey availability or spawning migrations. During periods of nutrient restriction, changes in metabolism occur to provide cellular energy via catabolic processes. Muscle is particularly affected by prolonged fasting as myofibrillar proteins act as a major energy source. To investigate the mechanisms of metabolic reorganisation with fasting and refeeding in a saltwater stage of Atlantic salmon (Salmo salar L.) we analysed the expression of genes involved in myogenesis, growth signalling, lipid biosynthesis and myofibrillar protein degradation and synthesis pathways using qPCR. Results: Hierarchical clustering of gene expression data revealed three clusters. The first cluster comprised genes involved in lipid metabolism and triacylglycerol synthesis (ALDOB, DGAT1 and LPL) which had peak expression 3-14d after refeeding. The second cluster comprised ADIPOQ, MLC2, IGF-I and TALDO1, with peak expression 14-32d after refeeding. Cluster III contained genes strongly down regulated as an initial response to feeding and included the ubiquitin ligases MuRF1 and MAFbx, myogenic regulatory factors and some metabolic genes. Conclusion: Early responses to refeeding in fasted salmon included the synthesis of triacylglycerols and activation of the adipogenic differentiation program. Inhibition of MuRF1 and MAFbx respectively may result in decreased degradation and concomitant increased production of myofibrillar proteins. Both of these processes preceded any increase in expression of myogenic regulatory factors and IGF-I. These responses could be a necessary strategy for an animal adapted to long periods of food deprivation whereby energy reserves are replenished prior to the resumption of myogenesis. |
format |
Article in Journal/Newspaper |
author |
Bower, Neil I. Taylor, Richard G. Johnston, Ian A. |
author_facet |
Bower, Neil I. Taylor, Richard G. Johnston, Ian A. |
author_sort |
Bower, Neil I. |
title |
Phasing of muscle gene expression with fasting-induced recovery growth in Atlantic salmon |
title_short |
Phasing of muscle gene expression with fasting-induced recovery growth in Atlantic salmon |
title_full |
Phasing of muscle gene expression with fasting-induced recovery growth in Atlantic salmon |
title_fullStr |
Phasing of muscle gene expression with fasting-induced recovery growth in Atlantic salmon |
title_full_unstemmed |
Phasing of muscle gene expression with fasting-induced recovery growth in Atlantic salmon |
title_sort |
phasing of muscle gene expression with fasting-induced recovery growth in atlantic salmon |
publishDate |
2009 |
url |
https://research-portal.st-andrews.ac.uk/en/researchoutput/phasing-of-muscle-gene-expression-with-fastinginduced-recovery-growth-in-atlantic-salmon(199319d1-163f-4ab9-9400-cd4b70fafb49).html https://doi.org/10.1186/1742-9994-6-18 https://research-repository.st-andrews.ac.uk/bitstream/10023/3328/1/Bower2009FrontiersZoology1742_9994_6_18.pdf http://www.scopus.com/inward/record.url?scp=69949106349&partnerID=8YFLogxK |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Bower , N I , Taylor , R G & Johnston , I A 2009 , ' Phasing of muscle gene expression with fasting-induced recovery growth in Atlantic salmon ' , Frontiers in Zoology , vol. 6 , 18 . https://doi.org/10.1186/1742-9994-6-18 |
op_relation |
https://research-portal.st-andrews.ac.uk/en/researchoutput/phasing-of-muscle-gene-expression-with-fastinginduced-recovery-growth-in-atlantic-salmon(199319d1-163f-4ab9-9400-cd4b70fafb49).html |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1186/1742-9994-6-18 |
container_title |
Frontiers in Zoology |
container_volume |
6 |
container_issue |
1 |
_version_ |
1802642413490012160 |