Switching to fast growth: the insulin-like growth factor (IGF) system in skeletal muscle of Atlantic salmon.
In this study we describe the complete coding sequence for insulin-like growth factor I (IGF-I), insulin-like growth factor II (IGF-II), insulin-like growth factor binding protein (IGFBP) 1, 2, 4, 5 and 6 and IGFBP-related protein 1 (IGFBP-rP1) of Atlantic salmon (Salmo salar L.). We also report the...
Published in: | Journal of Experimental Biology |
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Language: | English |
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2008
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Online Access: | https://research-portal.st-andrews.ac.uk/en/researchoutput/switching-to-fast-growth-the-insulinlike-growth-factor-igf-system-in-skeletal-muscle-of-atlantic-salmon(0e39f2af-ec85-4b28-983e-b938ec8effca).html https://doi.org/10.1242/jeb.024117 http://www.scopus.com/inward/record.url?scp=57449105454&partnerID=8YFLogxK |
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ftunstandrewcris:oai:research-portal.st-andrews.ac.uk:publications/0e39f2af-ec85-4b28-983e-b938ec8effca 2024-09-15T17:56:08+00:00 Switching to fast growth: the insulin-like growth factor (IGF) system in skeletal muscle of Atlantic salmon. Bower, Neil Ivan Li, Xuejun Taylor, R Johnston, Ian Alistair 2008-12-15 https://research-portal.st-andrews.ac.uk/en/researchoutput/switching-to-fast-growth-the-insulinlike-growth-factor-igf-system-in-skeletal-muscle-of-atlantic-salmon(0e39f2af-ec85-4b28-983e-b938ec8effca).html https://doi.org/10.1242/jeb.024117 http://www.scopus.com/inward/record.url?scp=57449105454&partnerID=8YFLogxK eng eng https://research-portal.st-andrews.ac.uk/en/researchoutput/switching-to-fast-growth-the-insulinlike-growth-factor-igf-system-in-skeletal-muscle-of-atlantic-salmon(0e39f2af-ec85-4b28-983e-b938ec8effca).html info:eu-repo/semantics/restrictedAccess Bower , N I , Li , X , Taylor , R & Johnston , I A 2008 , ' Switching to fast growth: the insulin-like growth factor (IGF) system in skeletal muscle of Atlantic salmon. ' , Journal of Experimental Biology , vol. 211 , no. 24 , pp. 3859-3870 . https://doi.org/10.1242/jeb.024117 IGF-I IGF-I receptor IGF binding proteins teleost fish growth skeletal muscle myogenesis rainbow trout coho salmon feeding nutrition TROUT ONCORHYNCHUS-MYKISS FACTOR BINDING PROTEIN-5 FACTOR-I GENE-EXPRESSION RAINBOW-TROUT SMOOTH-MUSCLE DIFFERENTIATION ZEBRAFISH RECEPTOR article 2008 ftunstandrewcris https://doi.org/10.1242/jeb.024117 2024-08-07T23:33:25Z In this study we describe the complete coding sequence for insulin-like growth factor I (IGF-I), insulin-like growth factor II (IGF-II), insulin-like growth factor binding protein (IGFBP) 1, 2, 4, 5 and 6 and IGFBP-related protein 1 (IGFBP-rP1) of Atlantic salmon (Salmo salar L.). We also report the characterisation of two gene paralogues of IGFBP-2 and IGFBP-5. Following 22 days restricted feeding (0d) to achieve zero growth, fish were fed to satiation and sampled at 3, 5, 7, 14, 30 and 60 days. Expression profiles for genes involved in the IGF signalling pathway in fast myotomal muscle were determined using real-time quantitative RT-PCR. The transition from zero to fast growth is characterised by constitutive upregulation of IGF-I and IGFBP-4, a transient increase in IGFBP-5.2, and downregulation of IGFBP-2.1, IGF-II, IGF2R (IGF-II receptor) and IGFR1a (IGF-I receptor a). Expression of IGFBP-2.2, IGFBP-5.1, IGFBP-6, IGFBP-rP1 and IGFR1b showed little or no response to feeding. Expression of the myogenic marker genes myogenin, MHC and MLC2 were higher with feed restriction, and decreased as an early response to feeding, before increasing to a peak at 14 days, corresponding with a peak in IGF-I expression. IGFBP-4, which contains a putative connective tissue localisation signal, was the only IGFBP constitutively upregulated following feeding, and was positively correlated with IGF-I expression. Together, these data show that switching to fast growth in Atlantic salmon skeletal muscle involves the local upregulation of IGF-I, IGFBP-5.2 and IGFBP-4, with downregulation of IGFBP-2.1. Article in Journal/Newspaper Atlantic salmon Salmo salar University of St Andrews: Research Portal Journal of Experimental Biology 211 24 3859 3870 |
institution |
Open Polar |
collection |
University of St Andrews: Research Portal |
op_collection_id |
ftunstandrewcris |
language |
English |
topic |
IGF-I IGF-I receptor IGF binding proteins teleost fish growth skeletal muscle myogenesis rainbow trout coho salmon feeding nutrition TROUT ONCORHYNCHUS-MYKISS FACTOR BINDING PROTEIN-5 FACTOR-I GENE-EXPRESSION RAINBOW-TROUT SMOOTH-MUSCLE DIFFERENTIATION ZEBRAFISH RECEPTOR |
spellingShingle |
IGF-I IGF-I receptor IGF binding proteins teleost fish growth skeletal muscle myogenesis rainbow trout coho salmon feeding nutrition TROUT ONCORHYNCHUS-MYKISS FACTOR BINDING PROTEIN-5 FACTOR-I GENE-EXPRESSION RAINBOW-TROUT SMOOTH-MUSCLE DIFFERENTIATION ZEBRAFISH RECEPTOR Bower, Neil Ivan Li, Xuejun Taylor, R Johnston, Ian Alistair Switching to fast growth: the insulin-like growth factor (IGF) system in skeletal muscle of Atlantic salmon. |
topic_facet |
IGF-I IGF-I receptor IGF binding proteins teleost fish growth skeletal muscle myogenesis rainbow trout coho salmon feeding nutrition TROUT ONCORHYNCHUS-MYKISS FACTOR BINDING PROTEIN-5 FACTOR-I GENE-EXPRESSION RAINBOW-TROUT SMOOTH-MUSCLE DIFFERENTIATION ZEBRAFISH RECEPTOR |
description |
In this study we describe the complete coding sequence for insulin-like growth factor I (IGF-I), insulin-like growth factor II (IGF-II), insulin-like growth factor binding protein (IGFBP) 1, 2, 4, 5 and 6 and IGFBP-related protein 1 (IGFBP-rP1) of Atlantic salmon (Salmo salar L.). We also report the characterisation of two gene paralogues of IGFBP-2 and IGFBP-5. Following 22 days restricted feeding (0d) to achieve zero growth, fish were fed to satiation and sampled at 3, 5, 7, 14, 30 and 60 days. Expression profiles for genes involved in the IGF signalling pathway in fast myotomal muscle were determined using real-time quantitative RT-PCR. The transition from zero to fast growth is characterised by constitutive upregulation of IGF-I and IGFBP-4, a transient increase in IGFBP-5.2, and downregulation of IGFBP-2.1, IGF-II, IGF2R (IGF-II receptor) and IGFR1a (IGF-I receptor a). Expression of IGFBP-2.2, IGFBP-5.1, IGFBP-6, IGFBP-rP1 and IGFR1b showed little or no response to feeding. Expression of the myogenic marker genes myogenin, MHC and MLC2 were higher with feed restriction, and decreased as an early response to feeding, before increasing to a peak at 14 days, corresponding with a peak in IGF-I expression. IGFBP-4, which contains a putative connective tissue localisation signal, was the only IGFBP constitutively upregulated following feeding, and was positively correlated with IGF-I expression. Together, these data show that switching to fast growth in Atlantic salmon skeletal muscle involves the local upregulation of IGF-I, IGFBP-5.2 and IGFBP-4, with downregulation of IGFBP-2.1. |
format |
Article in Journal/Newspaper |
author |
Bower, Neil Ivan Li, Xuejun Taylor, R Johnston, Ian Alistair |
author_facet |
Bower, Neil Ivan Li, Xuejun Taylor, R Johnston, Ian Alistair |
author_sort |
Bower, Neil Ivan |
title |
Switching to fast growth: the insulin-like growth factor (IGF) system in skeletal muscle of Atlantic salmon. |
title_short |
Switching to fast growth: the insulin-like growth factor (IGF) system in skeletal muscle of Atlantic salmon. |
title_full |
Switching to fast growth: the insulin-like growth factor (IGF) system in skeletal muscle of Atlantic salmon. |
title_fullStr |
Switching to fast growth: the insulin-like growth factor (IGF) system in skeletal muscle of Atlantic salmon. |
title_full_unstemmed |
Switching to fast growth: the insulin-like growth factor (IGF) system in skeletal muscle of Atlantic salmon. |
title_sort |
switching to fast growth: the insulin-like growth factor (igf) system in skeletal muscle of atlantic salmon. |
publishDate |
2008 |
url |
https://research-portal.st-andrews.ac.uk/en/researchoutput/switching-to-fast-growth-the-insulinlike-growth-factor-igf-system-in-skeletal-muscle-of-atlantic-salmon(0e39f2af-ec85-4b28-983e-b938ec8effca).html https://doi.org/10.1242/jeb.024117 http://www.scopus.com/inward/record.url?scp=57449105454&partnerID=8YFLogxK |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Bower , N I , Li , X , Taylor , R & Johnston , I A 2008 , ' Switching to fast growth: the insulin-like growth factor (IGF) system in skeletal muscle of Atlantic salmon. ' , Journal of Experimental Biology , vol. 211 , no. 24 , pp. 3859-3870 . https://doi.org/10.1242/jeb.024117 |
op_relation |
https://research-portal.st-andrews.ac.uk/en/researchoutput/switching-to-fast-growth-the-insulinlike-growth-factor-igf-system-in-skeletal-muscle-of-atlantic-salmon(0e39f2af-ec85-4b28-983e-b938ec8effca).html |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1242/jeb.024117 |
container_title |
Journal of Experimental Biology |
container_volume |
211 |
container_issue |
24 |
container_start_page |
3859 |
op_container_end_page |
3870 |
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1810432353294614528 |