The effects of dietary sodium loading on the activity and expression of Na, K-ATPase in the rectal gland of the European Dogfish (Scyliorhinus canicula)
cDNA fragments of both the alpha- and beta-subunits of the Na, K-ATPase and a cDNA fragment of the secretory form of Na-K-Cl cotransporter from the European dogfish (Scyliorhinus canicula) were amplified and cloned using degenerate primers in RT-PCR. These clones were used along with a sCFTR cDNA fr...
Published in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
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2002
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Online Access: | https://research-portal.st-andrews.ac.uk/en/researchoutput/the-effects-of-dietary-sodium-loading-on-the-activity-and-expression-of-na-katpase-in-the-rectal-gland-of-the-european-dogfish-scyliorhinus-canicula(00a814f0-5f48-421b-8a1e-01cca115aa07).html https://doi.org/10.1016/S1096-4959(01)00493-6 http://www.scopus.com/inward/record.url?scp=0036161514&partnerID=8YFLogxK |
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ftunstandrewcris:oai:research-portal.st-andrews.ac.uk:publications/00a814f0-5f48-421b-8a1e-01cca115aa07 2024-06-23T07:45:34+00:00 The effects of dietary sodium loading on the activity and expression of Na, K-ATPase in the rectal gland of the European Dogfish (Scyliorhinus canicula) MacKenzie, S Cutler, Christopher Paul Hazon, N Cramb, G 2002-02 https://research-portal.st-andrews.ac.uk/en/researchoutput/the-effects-of-dietary-sodium-loading-on-the-activity-and-expression-of-na-katpase-in-the-rectal-gland-of-the-european-dogfish-scyliorhinus-canicula(00a814f0-5f48-421b-8a1e-01cca115aa07).html https://doi.org/10.1016/S1096-4959(01)00493-6 http://www.scopus.com/inward/record.url?scp=0036161514&partnerID=8YFLogxK eng eng https://research-portal.st-andrews.ac.uk/en/researchoutput/the-effects-of-dietary-sodium-loading-on-the-activity-and-expression-of-na-katpase-in-the-rectal-gland-of-the-european-dogfish-scyliorhinus-canicula(00a814f0-5f48-421b-8a1e-01cca115aa07).html info:eu-repo/semantics/restrictedAccess MacKenzie , S , Cutler , C P , Hazon , N & Cramb , G 2002 , ' The effects of dietary sodium loading on the activity and expression of Na, K-ATPase in the rectal gland of the European Dogfish (Scyliorhinus canicula) ' , Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology , vol. 131 , no. 2 , pp. 185-200 . https://doi.org/10.1016/S1096-4959(01)00493-6 European dogfish Scyliorhinus canicula dietary salt Na K-ATPase CFTR Na,K,Cl-cotransporter expression PROTEIN-KINASE-C TRANSMEMBRANE CONDUCTANCE REGULATOR EEL ANGUILLA-ANGUILLA SMOOTH-MUSCLE CELLS SQUALUS-ACANTHIAS GENE-EXPRESSION COTRANSPORT PROTEIN HORMONAL-REGULATION CHLORIDE CHANNEL ISOLATED TUBULES article 2002 ftunstandrewcris https://doi.org/10.1016/S1096-4959(01)00493-6 2024-06-13T00:26:44Z cDNA fragments of both the alpha- and beta-subunits of the Na, K-ATPase and a cDNA fragment of the secretory form of Na-K-Cl cotransporter from the European dogfish (Scyliorhinus canicula) were amplified and cloned using degenerate primers in RT-PCR. These clones were used along with a sCFTR cDNA from the related dogfish shark, Squalus acanthias to characterise the expression of mRNAs for these ion transporters in the dogfish rectal gland subsequent to an acute feeding episode. Following a single feeding event where starved dogfish were fed squid portions (20 g squid/kg fish), there was a delayed and transient 40-fold increase in the activity of Na, K-ATPase in crude rectal gland homogenates. Increases in enzyme activity were apparent 3 h after the feeding event and peaked at 9 h before returning to control values within 24 h. These increases in activity were accompanied by small and transient decreases in plasma sodium and chloride concentrations lasting up to 3 days. Significant increases in the expression of mRNAs for alpha- and beta-subunits of the Na, K-ATPase, the Na-K-Cl cotransporter and CFTR chloride channel were detected but not until 1 -2 days after the feeding event. It is concluded that the transient increase in Na, K-ATPase activity is not attributable to increases in the abundance of alpha- and beta-subunit mRNAs but must be associated with some, as yet unknown, post-transcriptional activation mechanism. (C) 2002 Elsevier Science Inc. All rights reserved. Article in Journal/Newspaper Anguilla anguilla Squalus acanthias University of St Andrews: Research Portal Canicula ENVELOPE(-58.515,-58.515,-63.717,-63.717) Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 131 2 185 200 |
institution |
Open Polar |
collection |
University of St Andrews: Research Portal |
op_collection_id |
ftunstandrewcris |
language |
English |
topic |
European dogfish Scyliorhinus canicula dietary salt Na K-ATPase CFTR Na,K,Cl-cotransporter expression PROTEIN-KINASE-C TRANSMEMBRANE CONDUCTANCE REGULATOR EEL ANGUILLA-ANGUILLA SMOOTH-MUSCLE CELLS SQUALUS-ACANTHIAS GENE-EXPRESSION COTRANSPORT PROTEIN HORMONAL-REGULATION CHLORIDE CHANNEL ISOLATED TUBULES |
spellingShingle |
European dogfish Scyliorhinus canicula dietary salt Na K-ATPase CFTR Na,K,Cl-cotransporter expression PROTEIN-KINASE-C TRANSMEMBRANE CONDUCTANCE REGULATOR EEL ANGUILLA-ANGUILLA SMOOTH-MUSCLE CELLS SQUALUS-ACANTHIAS GENE-EXPRESSION COTRANSPORT PROTEIN HORMONAL-REGULATION CHLORIDE CHANNEL ISOLATED TUBULES MacKenzie, S Cutler, Christopher Paul Hazon, N Cramb, G The effects of dietary sodium loading on the activity and expression of Na, K-ATPase in the rectal gland of the European Dogfish (Scyliorhinus canicula) |
topic_facet |
European dogfish Scyliorhinus canicula dietary salt Na K-ATPase CFTR Na,K,Cl-cotransporter expression PROTEIN-KINASE-C TRANSMEMBRANE CONDUCTANCE REGULATOR EEL ANGUILLA-ANGUILLA SMOOTH-MUSCLE CELLS SQUALUS-ACANTHIAS GENE-EXPRESSION COTRANSPORT PROTEIN HORMONAL-REGULATION CHLORIDE CHANNEL ISOLATED TUBULES |
description |
cDNA fragments of both the alpha- and beta-subunits of the Na, K-ATPase and a cDNA fragment of the secretory form of Na-K-Cl cotransporter from the European dogfish (Scyliorhinus canicula) were amplified and cloned using degenerate primers in RT-PCR. These clones were used along with a sCFTR cDNA from the related dogfish shark, Squalus acanthias to characterise the expression of mRNAs for these ion transporters in the dogfish rectal gland subsequent to an acute feeding episode. Following a single feeding event where starved dogfish were fed squid portions (20 g squid/kg fish), there was a delayed and transient 40-fold increase in the activity of Na, K-ATPase in crude rectal gland homogenates. Increases in enzyme activity were apparent 3 h after the feeding event and peaked at 9 h before returning to control values within 24 h. These increases in activity were accompanied by small and transient decreases in plasma sodium and chloride concentrations lasting up to 3 days. Significant increases in the expression of mRNAs for alpha- and beta-subunits of the Na, K-ATPase, the Na-K-Cl cotransporter and CFTR chloride channel were detected but not until 1 -2 days after the feeding event. It is concluded that the transient increase in Na, K-ATPase activity is not attributable to increases in the abundance of alpha- and beta-subunit mRNAs but must be associated with some, as yet unknown, post-transcriptional activation mechanism. (C) 2002 Elsevier Science Inc. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
MacKenzie, S Cutler, Christopher Paul Hazon, N Cramb, G |
author_facet |
MacKenzie, S Cutler, Christopher Paul Hazon, N Cramb, G |
author_sort |
MacKenzie, S |
title |
The effects of dietary sodium loading on the activity and expression of Na, K-ATPase in the rectal gland of the European Dogfish (Scyliorhinus canicula) |
title_short |
The effects of dietary sodium loading on the activity and expression of Na, K-ATPase in the rectal gland of the European Dogfish (Scyliorhinus canicula) |
title_full |
The effects of dietary sodium loading on the activity and expression of Na, K-ATPase in the rectal gland of the European Dogfish (Scyliorhinus canicula) |
title_fullStr |
The effects of dietary sodium loading on the activity and expression of Na, K-ATPase in the rectal gland of the European Dogfish (Scyliorhinus canicula) |
title_full_unstemmed |
The effects of dietary sodium loading on the activity and expression of Na, K-ATPase in the rectal gland of the European Dogfish (Scyliorhinus canicula) |
title_sort |
effects of dietary sodium loading on the activity and expression of na, k-atpase in the rectal gland of the european dogfish (scyliorhinus canicula) |
publishDate |
2002 |
url |
https://research-portal.st-andrews.ac.uk/en/researchoutput/the-effects-of-dietary-sodium-loading-on-the-activity-and-expression-of-na-katpase-in-the-rectal-gland-of-the-european-dogfish-scyliorhinus-canicula(00a814f0-5f48-421b-8a1e-01cca115aa07).html https://doi.org/10.1016/S1096-4959(01)00493-6 http://www.scopus.com/inward/record.url?scp=0036161514&partnerID=8YFLogxK |
long_lat |
ENVELOPE(-58.515,-58.515,-63.717,-63.717) |
geographic |
Canicula |
geographic_facet |
Canicula |
genre |
Anguilla anguilla Squalus acanthias |
genre_facet |
Anguilla anguilla Squalus acanthias |
op_source |
MacKenzie , S , Cutler , C P , Hazon , N & Cramb , G 2002 , ' The effects of dietary sodium loading on the activity and expression of Na, K-ATPase in the rectal gland of the European Dogfish (Scyliorhinus canicula) ' , Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology , vol. 131 , no. 2 , pp. 185-200 . https://doi.org/10.1016/S1096-4959(01)00493-6 |
op_relation |
https://research-portal.st-andrews.ac.uk/en/researchoutput/the-effects-of-dietary-sodium-loading-on-the-activity-and-expression-of-na-katpase-in-the-rectal-gland-of-the-european-dogfish-scyliorhinus-canicula(00a814f0-5f48-421b-8a1e-01cca115aa07).html |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/S1096-4959(01)00493-6 |
container_title |
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
container_volume |
131 |
container_issue |
2 |
container_start_page |
185 |
op_container_end_page |
200 |
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1802641091342630912 |