A longitudinal study of autoantibodies against cytochrome P450 side-chain cleavage enzyme in dogs (Canis lupus familiaris) affected with hypoadrenocorticism (Addison's disease)
Autoantibodies directed against the P450 side chain cleavage enzyme (P450scc) have been recently described in dogs affected with hypoadrenocorticism, consistent with an immune-mediated pathogenesis of this endocrinopathy. In human autoimmune Addison’s disease, autoantibodies may have a predictive va...
Published in: | Veterinary Immunology and Immunopathology |
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Online Access: | http://eprints.nottingham.ac.uk/52132/ https://www.sciencedirect.com/science/article/pii/S0165242717303525 https://doi.org/10.1016/j.vetimm.2018.05.013 |
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ftunottingham:oai:eprints.nottingham.ac.uk:52132 2023-09-05T13:18:44+02:00 A longitudinal study of autoantibodies against cytochrome P450 side-chain cleavage enzyme in dogs (Canis lupus familiaris) affected with hypoadrenocorticism (Addison's disease) Boag, Alisdair M. Christie, Michael R. McLaughlin, Kerry A. Syme, Harriet M. Graham, Peter Catchpole, Brian 2018-05-26 http://eprints.nottingham.ac.uk/52132/ https://www.sciencedirect.com/science/article/pii/S0165242717303525 https://doi.org/10.1016/j.vetimm.2018.05.013 unknown Elsevier Boag, Alisdair M. and Christie, Michael R. and McLaughlin, Kerry A. and Syme, Harriet M. and Graham, Peter and Catchpole, Brian (2018) A longitudinal study of autoantibodies against cytochrome P450 side-chain cleavage enzyme in dogs (Canis lupus familiaris) affected with hypoadrenocorticism (Addison's disease). Veterinary Immunology and Immunopathology . ISSN 1873-2534 doi:10.1016/j.vetimm.2018.05.013 Article PeerReviewed 2018 ftunottingham https://doi.org/10.1016/j.vetimm.2018.05.013 2023-08-14T17:44:30Z Autoantibodies directed against the P450 side chain cleavage enzyme (P450scc) have been recently described in dogs affected with hypoadrenocorticism, consistent with an immune-mediated pathogenesis of this endocrinopathy. In human autoimmune Addison’s disease, autoantibodies may have a predictive value, being detectable before clinical signs developing, and have been shown to persist for a period of time after diagnosis. Furthermore, an autoantibody positive status post-diagnosis has been associated with successful remission of Addison’s disease following B-cell depletion, suggesting active immunopathology in these cases. The current study was designed to investigate changes in serum P450scc autoantibody status over time in dogs diagnosed with spontaneous hypoadrenocorticism. P450scc autoantibodies were measured using a species-specific radioimmunoprecipitation assay in an initial cohort of 213 dogs, indicating a prevalence of 24%. Thirty two of these dogs had repeat samples (n = 80 in total) available for analysis. Five dogs were consistently P450scc autoantibody positive in all samples, for up to 425 days following first sampling. Three dogs were initially autoantibody positive, then became seronegative at later time points. One dog, a 1 year old female entire standard poodle, was initially negative for P450scc autoantibodies, but seroconverted 18 months after diagnosis. The remaining 23 dogs with multiple samples available were consistently P450scc autoantibody negative. Persistence was not associated with sex (p = 0.673). This study demonstrates persistence of P450scc autoantibodies in a subset of dogs affected with hypoadrenocorticism and seroconversion over one year post-diagnosis. P450scc autoantibody reactivity in human autoimmune Addison’s disease has been associated with sex, with females having a higher prevalence, possibly due to P450scc expression in the ovary acting as an additional source of antigenic stimulation. However, there was no sex difference in autoantibody persistence in the dogs ... Article in Journal/Newspaper Canis lupus The University of Nottingham: Nottingham ePrints Veterinary Immunology and Immunopathology 202 41 45 |
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The University of Nottingham: Nottingham ePrints |
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ftunottingham |
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description |
Autoantibodies directed against the P450 side chain cleavage enzyme (P450scc) have been recently described in dogs affected with hypoadrenocorticism, consistent with an immune-mediated pathogenesis of this endocrinopathy. In human autoimmune Addison’s disease, autoantibodies may have a predictive value, being detectable before clinical signs developing, and have been shown to persist for a period of time after diagnosis. Furthermore, an autoantibody positive status post-diagnosis has been associated with successful remission of Addison’s disease following B-cell depletion, suggesting active immunopathology in these cases. The current study was designed to investigate changes in serum P450scc autoantibody status over time in dogs diagnosed with spontaneous hypoadrenocorticism. P450scc autoantibodies were measured using a species-specific radioimmunoprecipitation assay in an initial cohort of 213 dogs, indicating a prevalence of 24%. Thirty two of these dogs had repeat samples (n = 80 in total) available for analysis. Five dogs were consistently P450scc autoantibody positive in all samples, for up to 425 days following first sampling. Three dogs were initially autoantibody positive, then became seronegative at later time points. One dog, a 1 year old female entire standard poodle, was initially negative for P450scc autoantibodies, but seroconverted 18 months after diagnosis. The remaining 23 dogs with multiple samples available were consistently P450scc autoantibody negative. Persistence was not associated with sex (p = 0.673). This study demonstrates persistence of P450scc autoantibodies in a subset of dogs affected with hypoadrenocorticism and seroconversion over one year post-diagnosis. P450scc autoantibody reactivity in human autoimmune Addison’s disease has been associated with sex, with females having a higher prevalence, possibly due to P450scc expression in the ovary acting as an additional source of antigenic stimulation. However, there was no sex difference in autoantibody persistence in the dogs ... |
format |
Article in Journal/Newspaper |
author |
Boag, Alisdair M. Christie, Michael R. McLaughlin, Kerry A. Syme, Harriet M. Graham, Peter Catchpole, Brian |
spellingShingle |
Boag, Alisdair M. Christie, Michael R. McLaughlin, Kerry A. Syme, Harriet M. Graham, Peter Catchpole, Brian A longitudinal study of autoantibodies against cytochrome P450 side-chain cleavage enzyme in dogs (Canis lupus familiaris) affected with hypoadrenocorticism (Addison's disease) |
author_facet |
Boag, Alisdair M. Christie, Michael R. McLaughlin, Kerry A. Syme, Harriet M. Graham, Peter Catchpole, Brian |
author_sort |
Boag, Alisdair M. |
title |
A longitudinal study of autoantibodies against cytochrome P450 side-chain cleavage enzyme in dogs (Canis lupus familiaris) affected with hypoadrenocorticism (Addison's disease) |
title_short |
A longitudinal study of autoantibodies against cytochrome P450 side-chain cleavage enzyme in dogs (Canis lupus familiaris) affected with hypoadrenocorticism (Addison's disease) |
title_full |
A longitudinal study of autoantibodies against cytochrome P450 side-chain cleavage enzyme in dogs (Canis lupus familiaris) affected with hypoadrenocorticism (Addison's disease) |
title_fullStr |
A longitudinal study of autoantibodies against cytochrome P450 side-chain cleavage enzyme in dogs (Canis lupus familiaris) affected with hypoadrenocorticism (Addison's disease) |
title_full_unstemmed |
A longitudinal study of autoantibodies against cytochrome P450 side-chain cleavage enzyme in dogs (Canis lupus familiaris) affected with hypoadrenocorticism (Addison's disease) |
title_sort |
longitudinal study of autoantibodies against cytochrome p450 side-chain cleavage enzyme in dogs (canis lupus familiaris) affected with hypoadrenocorticism (addison's disease) |
publisher |
Elsevier |
publishDate |
2018 |
url |
http://eprints.nottingham.ac.uk/52132/ https://www.sciencedirect.com/science/article/pii/S0165242717303525 https://doi.org/10.1016/j.vetimm.2018.05.013 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
Boag, Alisdair M. and Christie, Michael R. and McLaughlin, Kerry A. and Syme, Harriet M. and Graham, Peter and Catchpole, Brian (2018) A longitudinal study of autoantibodies against cytochrome P450 side-chain cleavage enzyme in dogs (Canis lupus familiaris) affected with hypoadrenocorticism (Addison's disease). Veterinary Immunology and Immunopathology . ISSN 1873-2534 doi:10.1016/j.vetimm.2018.05.013 |
op_doi |
https://doi.org/10.1016/j.vetimm.2018.05.013 |
container_title |
Veterinary Immunology and Immunopathology |
container_volume |
202 |
container_start_page |
41 |
op_container_end_page |
45 |
_version_ |
1776199615730679808 |