Interpreting ELISA analyses from wild animal samples: Some recurrent issues and solutions
International audience Many studies in disease and ecological immunology rely on the use of assays thatquantify the amount of specific antibodies (immunoglobulin) in samples. Enzymelinkedimmunosorbent assays (ELISAs) are increasingly used in ecology due to theiravailability for a broad array of anti...
Published in: | Functional Ecology |
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Main Authors: | , , , , , , , |
Other Authors: | , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2017
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Subjects: | |
Online Access: | https://hal.science/hal-01882627 https://hal.science/hal-01882627/document https://hal.science/hal-01882627/file/672476.pdf https://doi.org/10.1111/1365-2435.12942 |
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HAL Portal Paul-Valéry University Montpellier 3 |
op_collection_id |
ftunmontpellier3 |
language |
English |
topic |
Newcastle disease virus immuno-ecology disease ecology ecological immunology vaccination seabirds Borrelia ELISA [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health [SDE.IE]Environmental Sciences/Environmental Engineering [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity [SDV.IMM.VAC]Life Sciences [q-bio]/Immunology/Vaccinology |
spellingShingle |
Newcastle disease virus immuno-ecology disease ecology ecological immunology vaccination seabirds Borrelia ELISA [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health [SDE.IE]Environmental Sciences/Environmental Engineering [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity [SDV.IMM.VAC]Life Sciences [q-bio]/Immunology/Vaccinology Garnier, Romain Ramos, Raül Sanz-Aguilar, Ana Poisbleau, Maud Weimerskirch, Henri Burthe, Sarah Tornos, Jérémy Boulinier, Thierry Interpreting ELISA analyses from wild animal samples: Some recurrent issues and solutions |
topic_facet |
Newcastle disease virus immuno-ecology disease ecology ecological immunology vaccination seabirds Borrelia ELISA [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health [SDE.IE]Environmental Sciences/Environmental Engineering [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity [SDV.IMM.VAC]Life Sciences [q-bio]/Immunology/Vaccinology |
description |
International audience Many studies in disease and ecological immunology rely on the use of assays thatquantify the amount of specific antibodies (immunoglobulin) in samples. Enzymelinkedimmunosorbent assays (ELISAs) are increasingly used in ecology due to theiravailability for a broad array of antigens and the limited amount of sampling materialthey require. Two recurrent methodological issues are nevertheless faced byresearchers: (1) the limited availability of immunological assays and reagents developedfor non-model species, and (2) the statistical determination of the cut-offthreshold used to distinguish individual samples that are likely to have or not tohave antibodies against a specific antigen.Here, we outline two solutions to deal with these issues. First, we show that implementingtwo assays with differing detection methods can help validate the use ofreagents, such as antibodies, in species different from their intended target. We illustrate this by comparing the quantification of specific vaccinal antibodies against Newcastle disease virus (NDV) using two ELISA approaches in four seabird species (Cory’s shearwater, European shag, European storm petrel and Southern rockhopper penguin).Second, we provide a simple way to determine from the distribution of ELISA valueswhether the assayed samples are likely to be made of a single group of individuals(likely negative) or of two groups of individuals (negative and positive). We illustrate the use of this approach with two independent data sets: NDV antibody levels following vaccination and anti-Borrelia antibody levels following natural exposure.The practical implementation of these methodological approaches could provide away to efficiently apply ELISAs and other immune-based assays to address questions in the growing fields of ecological immunology and disease ecology |
author2 |
Department of Veterinary Medicine, Disease Dynamics Unit UK University of Cambridge UK (CAM) Universitat de Barcelona (UB) Institut Mediterrani d'Estudis Avancats (IMEDEA) Consejo Superior de Investigaciones Cientificas España = Spanish National Research Council Spain (CSIC)-Universitat de les Illes Balears = Universidad de las Islas Baleares = University of the Balearic Islands (UIB) Department of Biology, Behavioural Ecology and Ecophysiology Group University of Antwerp (UA) Centre d'Études Biologiques de Chizé - UMR 7372 (CEBC) Institut National de la Recherche Agronomique (INRA)-La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS) Centre for Ecology & Hydrology Midlothian, UK) Centre d’Ecologie Fonctionnelle et Evolutive (CEFE) Université Paul-Valéry - Montpellier 3 (UPVM)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École Pratique des Hautes Études (EPHE) Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD France-Sud )-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) |
format |
Article in Journal/Newspaper |
author |
Garnier, Romain Ramos, Raül Sanz-Aguilar, Ana Poisbleau, Maud Weimerskirch, Henri Burthe, Sarah Tornos, Jérémy Boulinier, Thierry |
author_facet |
Garnier, Romain Ramos, Raül Sanz-Aguilar, Ana Poisbleau, Maud Weimerskirch, Henri Burthe, Sarah Tornos, Jérémy Boulinier, Thierry |
author_sort |
Garnier, Romain |
title |
Interpreting ELISA analyses from wild animal samples: Some recurrent issues and solutions |
title_short |
Interpreting ELISA analyses from wild animal samples: Some recurrent issues and solutions |
title_full |
Interpreting ELISA analyses from wild animal samples: Some recurrent issues and solutions |
title_fullStr |
Interpreting ELISA analyses from wild animal samples: Some recurrent issues and solutions |
title_full_unstemmed |
Interpreting ELISA analyses from wild animal samples: Some recurrent issues and solutions |
title_sort |
interpreting elisa analyses from wild animal samples: some recurrent issues and solutions |
publisher |
HAL CCSD |
publishDate |
2017 |
url |
https://hal.science/hal-01882627 https://hal.science/hal-01882627/document https://hal.science/hal-01882627/file/672476.pdf https://doi.org/10.1111/1365-2435.12942 |
genre |
Rockhopper penguin |
genre_facet |
Rockhopper penguin |
op_source |
ISSN: 0269-8463 EISSN: 1365-2435 Functional Ecology https://hal.science/hal-01882627 Functional Ecology, 2017, 31 (12), pp.2255 - 2262. ⟨10.1111/1365-2435.12942⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/1365-2435.12942 hal-01882627 https://hal.science/hal-01882627 https://hal.science/hal-01882627/document https://hal.science/hal-01882627/file/672476.pdf doi:10.1111/1365-2435.12942 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1111/1365-2435.12942 |
container_title |
Functional Ecology |
container_volume |
31 |
container_issue |
12 |
container_start_page |
2255 |
op_container_end_page |
2262 |
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1799488307812368384 |
spelling |
ftunmontpellier3:oai:HAL:hal-01882627v1 2024-05-19T07:47:50+00:00 Interpreting ELISA analyses from wild animal samples: Some recurrent issues and solutions Garnier, Romain Ramos, Raül Sanz-Aguilar, Ana Poisbleau, Maud Weimerskirch, Henri Burthe, Sarah Tornos, Jérémy Boulinier, Thierry Department of Veterinary Medicine, Disease Dynamics Unit UK University of Cambridge UK (CAM) Universitat de Barcelona (UB) Institut Mediterrani d'Estudis Avancats (IMEDEA) Consejo Superior de Investigaciones Cientificas España = Spanish National Research Council Spain (CSIC)-Universitat de les Illes Balears = Universidad de las Islas Baleares = University of the Balearic Islands (UIB) Department of Biology, Behavioural Ecology and Ecophysiology Group University of Antwerp (UA) Centre d'Études Biologiques de Chizé - UMR 7372 (CEBC) Institut National de la Recherche Agronomique (INRA)-La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS) Centre for Ecology & Hydrology Midlothian, UK) Centre d’Ecologie Fonctionnelle et Evolutive (CEFE) Université Paul-Valéry - Montpellier 3 (UPVM)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École Pratique des Hautes Études (EPHE) Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD France-Sud )-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) 2017 https://hal.science/hal-01882627 https://hal.science/hal-01882627/document https://hal.science/hal-01882627/file/672476.pdf https://doi.org/10.1111/1365-2435.12942 en eng HAL CCSD Wiley info:eu-repo/semantics/altIdentifier/doi/10.1111/1365-2435.12942 hal-01882627 https://hal.science/hal-01882627 https://hal.science/hal-01882627/document https://hal.science/hal-01882627/file/672476.pdf doi:10.1111/1365-2435.12942 info:eu-repo/semantics/OpenAccess ISSN: 0269-8463 EISSN: 1365-2435 Functional Ecology https://hal.science/hal-01882627 Functional Ecology, 2017, 31 (12), pp.2255 - 2262. ⟨10.1111/1365-2435.12942⟩ Newcastle disease virus immuno-ecology disease ecology ecological immunology vaccination seabirds Borrelia ELISA [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health [SDE.IE]Environmental Sciences/Environmental Engineering [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity [SDV.IMM.VAC]Life Sciences [q-bio]/Immunology/Vaccinology info:eu-repo/semantics/article Journal articles 2017 ftunmontpellier3 https://doi.org/10.1111/1365-2435.12942 2024-04-22T16:57:07Z International audience Many studies in disease and ecological immunology rely on the use of assays thatquantify the amount of specific antibodies (immunoglobulin) in samples. Enzymelinkedimmunosorbent assays (ELISAs) are increasingly used in ecology due to theiravailability for a broad array of antigens and the limited amount of sampling materialthey require. Two recurrent methodological issues are nevertheless faced byresearchers: (1) the limited availability of immunological assays and reagents developedfor non-model species, and (2) the statistical determination of the cut-offthreshold used to distinguish individual samples that are likely to have or not tohave antibodies against a specific antigen.Here, we outline two solutions to deal with these issues. First, we show that implementingtwo assays with differing detection methods can help validate the use ofreagents, such as antibodies, in species different from their intended target. We illustrate this by comparing the quantification of specific vaccinal antibodies against Newcastle disease virus (NDV) using two ELISA approaches in four seabird species (Cory’s shearwater, European shag, European storm petrel and Southern rockhopper penguin).Second, we provide a simple way to determine from the distribution of ELISA valueswhether the assayed samples are likely to be made of a single group of individuals(likely negative) or of two groups of individuals (negative and positive). We illustrate the use of this approach with two independent data sets: NDV antibody levels following vaccination and anti-Borrelia antibody levels following natural exposure.The practical implementation of these methodological approaches could provide away to efficiently apply ELISAs and other immune-based assays to address questions in the growing fields of ecological immunology and disease ecology Article in Journal/Newspaper Rockhopper penguin HAL Portal Paul-Valéry University Montpellier 3 Functional Ecology 31 12 2255 2262 |