Signal transduction in primary human T lymphocytes in altered gravity - results of the MASER-12 suborbital space flight mission
We investigated the influence of altered gravity on key proteins of T cell activation during the MASER-12 ballistic suborbital rocket mission of the European Space Agency (ESA) and the Swedish Space Cooperation (SSC) at ESRANGE Space Center (Kiruna, Sweden). We quantified components of the T cell re...
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BioMed Central
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ftunivzuerich:oai:www.zora.uzh.ch:93286 2024-10-13T14:08:47+00:00 Signal transduction in primary human T lymphocytes in altered gravity - results of the MASER-12 suborbital space flight mission Tauber, Svantje Hauschild, Swantje Crescio, Claudia Secchi, Christian Paulsen, Katrin Pantaleo, Antonella Saba, Angela Buttron, Isabell Thiel, Cora Sandra Cogoli, Augusto Pippia, Proto Ullrich, Oliver 2013-05-07 application/pdf https://www.zora.uzh.ch/id/eprint/93286/ https://www.zora.uzh.ch/id/eprint/93286/1/Tauber_et_al._2013.pdf https://doi.org/10.5167/uzh-93286 https://doi.org/10.1186/1478-811X-11-32 eng eng BioMed Central https://www.zora.uzh.ch/id/eprint/93286/1/Tauber_et_al._2013.pdf doi:10.5167/uzh-93286 doi:10.1186/1478-811X-11-32 info:pmid/23651740 urn:issn:1478-811X info:eu-repo/semantics/openAccess Creative Commons: Attribution 2.0 Generic (CC BY 2.0) http://creativecommons.org/licenses/by/2.0/ Tauber, Svantje; Hauschild, Swantje; Crescio, Claudia; Secchi, Christian; Paulsen, Katrin; Pantaleo, Antonella; Saba, Angela; Buttron, Isabell; Thiel, Cora Sandra; Cogoli, Augusto; Pippia, Proto; Ullrich, Oliver (2013). Signal transduction in primary human T lymphocytes in altered gravity - results of the MASER-12 suborbital space flight mission. Cell Communication and Signaling, 11:32. Institute of Anatomy Zurich Center for Integrative Human Physiology (ZIHP) 570 Life sciences biology 610 Medicine & health Journal Article PeerReviewed info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2013 ftunivzuerich https://doi.org/10.5167/uzh-9328610.1186/1478-811X-11-32 2024-09-18T00:49:46Z We investigated the influence of altered gravity on key proteins of T cell activation during the MASER-12 ballistic suborbital rocket mission of the European Space Agency (ESA) and the Swedish Space Cooperation (SSC) at ESRANGE Space Center (Kiruna, Sweden). We quantified components of the T cell receptor, the membrane proximal signaling, MAPK-signaling, IL-2R, histone modifications and the cytoskeleton in non-activated and in ConA/CD28-activated primary human T lymphocytes. The hypergravity phase during the launch resulted in a downregulation of the IL-2 and CD3 receptor and reduction of tyrosine phosphorylation, p44/42-MAPK phosphorylation and histone H3 acetylation, whereas LAT phosphorylation was increased. Compared to the baseline situation at the point of entry into the microgravity phase, CD3 and IL-2 receptor expression at the surface of non-activated T cells were reduced after 6 min microgravity. Importantly, p44/42-MAPK-phosphorylation was also reduced after 6 min microgravity compared to the 1g ground controls, but also in direct comparison between the in-flight μg and the 1g group. In activated T cells, the reduced CD3 and IL-2 receptor expression at the baseline situation recovered significantly during in-flight 1g conditions, but not during microgravity conditions. Beta-tubulin increased significantly after onset of microgravity until the end of the microgravity phase, but not in the in-flight 1g condition. This study suggests that key proteins of T cell signal modules are not severely disturbed in microgravity. Instead, it can be supposed that the strong T cell inhibiting signal occurs downstream from membrane proximal signaling, such as at the transcriptional level as described recently. However, the MASER-12 experiment could identify signal molecules, which are sensitive to altered gravity, and indicates that gravity is obviously not only a requirement for transcriptional processes as described before, but also for specific phosphorylation / dephosphorylation of signal molecules and surface ... Article in Journal/Newspaper Kiruna University of Zurich (UZH): ZORA (Zurich Open Repository and Archive Esrange ENVELOPE(21.117,21.117,67.883,67.883) Kiruna |
institution |
Open Polar |
collection |
University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
op_collection_id |
ftunivzuerich |
language |
English |
topic |
Institute of Anatomy Zurich Center for Integrative Human Physiology (ZIHP) 570 Life sciences biology 610 Medicine & health |
spellingShingle |
Institute of Anatomy Zurich Center for Integrative Human Physiology (ZIHP) 570 Life sciences biology 610 Medicine & health Tauber, Svantje Hauschild, Swantje Crescio, Claudia Secchi, Christian Paulsen, Katrin Pantaleo, Antonella Saba, Angela Buttron, Isabell Thiel, Cora Sandra Cogoli, Augusto Pippia, Proto Ullrich, Oliver Signal transduction in primary human T lymphocytes in altered gravity - results of the MASER-12 suborbital space flight mission |
topic_facet |
Institute of Anatomy Zurich Center for Integrative Human Physiology (ZIHP) 570 Life sciences biology 610 Medicine & health |
description |
We investigated the influence of altered gravity on key proteins of T cell activation during the MASER-12 ballistic suborbital rocket mission of the European Space Agency (ESA) and the Swedish Space Cooperation (SSC) at ESRANGE Space Center (Kiruna, Sweden). We quantified components of the T cell receptor, the membrane proximal signaling, MAPK-signaling, IL-2R, histone modifications and the cytoskeleton in non-activated and in ConA/CD28-activated primary human T lymphocytes. The hypergravity phase during the launch resulted in a downregulation of the IL-2 and CD3 receptor and reduction of tyrosine phosphorylation, p44/42-MAPK phosphorylation and histone H3 acetylation, whereas LAT phosphorylation was increased. Compared to the baseline situation at the point of entry into the microgravity phase, CD3 and IL-2 receptor expression at the surface of non-activated T cells were reduced after 6 min microgravity. Importantly, p44/42-MAPK-phosphorylation was also reduced after 6 min microgravity compared to the 1g ground controls, but also in direct comparison between the in-flight μg and the 1g group. In activated T cells, the reduced CD3 and IL-2 receptor expression at the baseline situation recovered significantly during in-flight 1g conditions, but not during microgravity conditions. Beta-tubulin increased significantly after onset of microgravity until the end of the microgravity phase, but not in the in-flight 1g condition. This study suggests that key proteins of T cell signal modules are not severely disturbed in microgravity. Instead, it can be supposed that the strong T cell inhibiting signal occurs downstream from membrane proximal signaling, such as at the transcriptional level as described recently. However, the MASER-12 experiment could identify signal molecules, which are sensitive to altered gravity, and indicates that gravity is obviously not only a requirement for transcriptional processes as described before, but also for specific phosphorylation / dephosphorylation of signal molecules and surface ... |
format |
Article in Journal/Newspaper |
author |
Tauber, Svantje Hauschild, Swantje Crescio, Claudia Secchi, Christian Paulsen, Katrin Pantaleo, Antonella Saba, Angela Buttron, Isabell Thiel, Cora Sandra Cogoli, Augusto Pippia, Proto Ullrich, Oliver |
author_facet |
Tauber, Svantje Hauschild, Swantje Crescio, Claudia Secchi, Christian Paulsen, Katrin Pantaleo, Antonella Saba, Angela Buttron, Isabell Thiel, Cora Sandra Cogoli, Augusto Pippia, Proto Ullrich, Oliver |
author_sort |
Tauber, Svantje |
title |
Signal transduction in primary human T lymphocytes in altered gravity - results of the MASER-12 suborbital space flight mission |
title_short |
Signal transduction in primary human T lymphocytes in altered gravity - results of the MASER-12 suborbital space flight mission |
title_full |
Signal transduction in primary human T lymphocytes in altered gravity - results of the MASER-12 suborbital space flight mission |
title_fullStr |
Signal transduction in primary human T lymphocytes in altered gravity - results of the MASER-12 suborbital space flight mission |
title_full_unstemmed |
Signal transduction in primary human T lymphocytes in altered gravity - results of the MASER-12 suborbital space flight mission |
title_sort |
signal transduction in primary human t lymphocytes in altered gravity - results of the maser-12 suborbital space flight mission |
publisher |
BioMed Central |
publishDate |
2013 |
url |
https://www.zora.uzh.ch/id/eprint/93286/ https://www.zora.uzh.ch/id/eprint/93286/1/Tauber_et_al._2013.pdf https://doi.org/10.5167/uzh-93286 https://doi.org/10.1186/1478-811X-11-32 |
long_lat |
ENVELOPE(21.117,21.117,67.883,67.883) |
geographic |
Esrange Kiruna |
geographic_facet |
Esrange Kiruna |
genre |
Kiruna |
genre_facet |
Kiruna |
op_source |
Tauber, Svantje; Hauschild, Swantje; Crescio, Claudia; Secchi, Christian; Paulsen, Katrin; Pantaleo, Antonella; Saba, Angela; Buttron, Isabell; Thiel, Cora Sandra; Cogoli, Augusto; Pippia, Proto; Ullrich, Oliver (2013). Signal transduction in primary human T lymphocytes in altered gravity - results of the MASER-12 suborbital space flight mission. Cell Communication and Signaling, 11:32. |
op_relation |
https://www.zora.uzh.ch/id/eprint/93286/1/Tauber_et_al._2013.pdf doi:10.5167/uzh-93286 doi:10.1186/1478-811X-11-32 info:pmid/23651740 urn:issn:1478-811X |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons: Attribution 2.0 Generic (CC BY 2.0) http://creativecommons.org/licenses/by/2.0/ |
op_doi |
https://doi.org/10.5167/uzh-9328610.1186/1478-811X-11-32 |
_version_ |
1812815541818097664 |