Individual filamentous phage imaged by electron holography
An in-line electron hologram of an individual f1.K phage was recorded with a purpose-built low energy electron point source (LEEPS) microscope. Cryo-microscopic methods were employed to prepare the specimen so that a single phage could be presented to the coherent low energy electrons: An aqueous ph...
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ftunivzuerich:oai:www.zora.uzh.ch:50010 2024-10-20T14:09:29+00:00 Individual filamentous phage imaged by electron holography Stevens, G B Krüger, M Latychevskaia, T Lindner, P Plückthun, A Fink, H-W 2011 application/pdf https://www.zora.uzh.ch/id/eprint/50010/ https://www.zora.uzh.ch/id/eprint/50010/1/Stevens_et_al._2011.pdf https://www.zora.uzh.ch/id/eprint/50010/12/ZORA_NL_50010.pdf eng eng Springer https://www.zora.uzh.ch/id/eprint/50010/1/Stevens_et_al._2011.pdf https://www.zora.uzh.ch/id/eprint/50010/12/ZORA_NL_50010.pdf doi:10.5167/uzh-50010 doi:10.1007/s00249-011-0743-y info:pmid/21874382 urn:issn:0175-7571 info:eu-repo/semantics/openAccess Stevens, G B; Krüger, M; Latychevskaia, T; Lindner, P; Plückthun, A; Fink, H-W (2011). Individual filamentous phage imaged by electron holography. European Biophysics Journal, 40(10):1197-1201. Department of Biochemistry Physics Institute 570 Life sciences biology 530 Physics Journal Article PeerReviewed info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2011 ftunivzuerich https://doi.org/10.5167/uzh-5001010.1007/s00249-011-0743-y 2024-10-09T15:01:16Z An in-line electron hologram of an individual f1.K phage was recorded with a purpose-built low energy electron point source (LEEPS) microscope. Cryo-microscopic methods were employed to prepare the specimen so that a single phage could be presented to the coherent low energy electrons: An aqueous phage suspension was applied to a thin carbon membrane with micro-machined slits. The membrane was rapidly cooled to freeze the remaining water as an amorphous ice sheet, which was then sublimated at low temperatures and pressures to leave individual free-standing phages suspended across slits. An image of a phage particle, depicted as the amplitude of the object wave, was reconstructed numerically from a digitized record of the hologram, obtained using 88 eV coherent electrons. The reconstructed image shows a single phage suspended across a slit in a supporting carbon membrane, magnified by a factor of 100,000. The width and shape in the reconstructed image compared well with a TEM image of the same filament. It is thus possible to record and reconstruct electron holograms of an individual phage. The challenge now is to improve the resolution of reconstructed images obtained by this method and to extend these structural studies to other biological molecules. Article in Journal/Newspaper Ice Sheet University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
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Open Polar |
collection |
University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
op_collection_id |
ftunivzuerich |
language |
English |
topic |
Department of Biochemistry Physics Institute 570 Life sciences biology 530 Physics |
spellingShingle |
Department of Biochemistry Physics Institute 570 Life sciences biology 530 Physics Stevens, G B Krüger, M Latychevskaia, T Lindner, P Plückthun, A Fink, H-W Individual filamentous phage imaged by electron holography |
topic_facet |
Department of Biochemistry Physics Institute 570 Life sciences biology 530 Physics |
description |
An in-line electron hologram of an individual f1.K phage was recorded with a purpose-built low energy electron point source (LEEPS) microscope. Cryo-microscopic methods were employed to prepare the specimen so that a single phage could be presented to the coherent low energy electrons: An aqueous phage suspension was applied to a thin carbon membrane with micro-machined slits. The membrane was rapidly cooled to freeze the remaining water as an amorphous ice sheet, which was then sublimated at low temperatures and pressures to leave individual free-standing phages suspended across slits. An image of a phage particle, depicted as the amplitude of the object wave, was reconstructed numerically from a digitized record of the hologram, obtained using 88 eV coherent electrons. The reconstructed image shows a single phage suspended across a slit in a supporting carbon membrane, magnified by a factor of 100,000. The width and shape in the reconstructed image compared well with a TEM image of the same filament. It is thus possible to record and reconstruct electron holograms of an individual phage. The challenge now is to improve the resolution of reconstructed images obtained by this method and to extend these structural studies to other biological molecules. |
format |
Article in Journal/Newspaper |
author |
Stevens, G B Krüger, M Latychevskaia, T Lindner, P Plückthun, A Fink, H-W |
author_facet |
Stevens, G B Krüger, M Latychevskaia, T Lindner, P Plückthun, A Fink, H-W |
author_sort |
Stevens, G B |
title |
Individual filamentous phage imaged by electron holography |
title_short |
Individual filamentous phage imaged by electron holography |
title_full |
Individual filamentous phage imaged by electron holography |
title_fullStr |
Individual filamentous phage imaged by electron holography |
title_full_unstemmed |
Individual filamentous phage imaged by electron holography |
title_sort |
individual filamentous phage imaged by electron holography |
publisher |
Springer |
publishDate |
2011 |
url |
https://www.zora.uzh.ch/id/eprint/50010/ https://www.zora.uzh.ch/id/eprint/50010/1/Stevens_et_al._2011.pdf https://www.zora.uzh.ch/id/eprint/50010/12/ZORA_NL_50010.pdf |
genre |
Ice Sheet |
genre_facet |
Ice Sheet |
op_source |
Stevens, G B; Krüger, M; Latychevskaia, T; Lindner, P; Plückthun, A; Fink, H-W (2011). Individual filamentous phage imaged by electron holography. European Biophysics Journal, 40(10):1197-1201. |
op_relation |
https://www.zora.uzh.ch/id/eprint/50010/1/Stevens_et_al._2011.pdf https://www.zora.uzh.ch/id/eprint/50010/12/ZORA_NL_50010.pdf doi:10.5167/uzh-50010 doi:10.1007/s00249-011-0743-y info:pmid/21874382 urn:issn:0175-7571 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.5167/uzh-5001010.1007/s00249-011-0743-y |
_version_ |
1813449007663415296 |