Antigestagens Mediate the Expression of Decidualization Markers, Extracellular Matrix Factors and Connexin 43 in Decidualized Dog Uterine Stromal (DUS) Cells
Feto-maternal communication in the dog involves the differentiation of stromal cells into decidual cells. As the only placental cells expressing the nuclear progesterone (P4) receptor (PGR), decidual cells play crucial roles in the maintenance and termination of pregnancy. Accordingly, to investigat...
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Online Access: | https://www.zora.uzh.ch/id/eprint/223473/ https://www.zora.uzh.ch/id/eprint/223473/1/animals_12_00798.pdf https://doi.org/10.5167/uzh-223473 https://doi.org/10.3390/ani12070798 |
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ftunivzuerich:oai:www.zora.uzh.ch:223473 2024-09-15T18:01:20+00:00 Antigestagens Mediate the Expression of Decidualization Markers, Extracellular Matrix Factors and Connexin 43 in Decidualized Dog Uterine Stromal (DUS) Cells Kazemian, Ali Tavares Pereira, Miguel Hoffmann, Bernd Kowalewski, Mariusz P 2022-03-22 application/pdf https://www.zora.uzh.ch/id/eprint/223473/ https://www.zora.uzh.ch/id/eprint/223473/1/animals_12_00798.pdf https://doi.org/10.5167/uzh-223473 https://doi.org/10.3390/ani12070798 eng eng MDPI Publishing https://www.zora.uzh.ch/id/eprint/223473/1/animals_12_00798.pdf doi:10.5167/uzh-223473 doi:10.3390/ani12070798 info:pmid/35405788 urn:issn:2076-2615 info:eu-repo/semantics/openAccess Creative Commons: Attribution 4.0 International (CC BY 4.0) http://creativecommons.org/licenses/by/4.0/ Kazemian, Ali; Tavares Pereira, Miguel; Hoffmann, Bernd; Kowalewski, Mariusz P (2022). Antigestagens Mediate the Expression of Decidualization Markers, Extracellular Matrix Factors and Connexin 43 in Decidualized Dog Uterine Stromal (DUS) Cells. Animals, 12(7):798. Institute of Veterinary Anatomy Center for Clinical Studies 570 Life sciences biology General Veterinary Animal Science and Zoology dog (Canis lupus familiaris) dog uterine stromal (DUS) cells decidualization nuclear progesterone receptor (PGR) antigestagens Journal Article PeerReviewed info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2022 ftunivzuerich https://doi.org/10.5167/uzh-22347310.3390/ani12070798 2024-09-04T00:39:07Z Feto-maternal communication in the dog involves the differentiation of stromal cells into decidual cells. As the only placental cells expressing the nuclear progesterone (P4) receptor (PGR), decidual cells play crucial roles in the maintenance and termination of pregnancy. Accordingly, to investigate possible PGR-mediated mechanisms in canine decidual cells, in vitro decidualized dog uterine stromal (DUS) cells were treated with functional PGR-blockers, mifepristone and aglepristone. Effects on decidualization markers, epithelial and mesenchymal factors, and markers of cellular viability were assessed. Decidualization increased the expression of PTGES, PGR, IGF1, and PRLR, along with ECM1, COL4 and CX43, but downregulated IGF2. DUS cells retained their mesenchymal character, and the expression of COL4 indicated the mesenchymal-epithelial transformation. Antigestagen treatment decreased the availability of PTGES, PRLR, IGF1 and PGR. Furthermore, antigestagens decreased the mRNA and protein expression of CX43, and transcriptional levels of ECM1 and COL4. Additionally, antigestagens increased levels of activated-CASP3 (a proapoptotic factor), associated with lowered levels of PCNA (a proliferation marker). These data reveal important aspects of the functional involvement of PGR in canine decidual cells, regarding the expression of decidualization markers and acquisition of epithelial-like characteristics. Some of these mechanisms may be crucial for the maintenance and/or termination of canine pregnancy. Article in Journal/Newspaper Canis lupus University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
institution |
Open Polar |
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University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
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ftunivzuerich |
language |
English |
topic |
Institute of Veterinary Anatomy Center for Clinical Studies 570 Life sciences biology General Veterinary Animal Science and Zoology dog (Canis lupus familiaris) dog uterine stromal (DUS) cells decidualization nuclear progesterone receptor (PGR) antigestagens |
spellingShingle |
Institute of Veterinary Anatomy Center for Clinical Studies 570 Life sciences biology General Veterinary Animal Science and Zoology dog (Canis lupus familiaris) dog uterine stromal (DUS) cells decidualization nuclear progesterone receptor (PGR) antigestagens Kazemian, Ali Tavares Pereira, Miguel Hoffmann, Bernd Kowalewski, Mariusz P Antigestagens Mediate the Expression of Decidualization Markers, Extracellular Matrix Factors and Connexin 43 in Decidualized Dog Uterine Stromal (DUS) Cells |
topic_facet |
Institute of Veterinary Anatomy Center for Clinical Studies 570 Life sciences biology General Veterinary Animal Science and Zoology dog (Canis lupus familiaris) dog uterine stromal (DUS) cells decidualization nuclear progesterone receptor (PGR) antigestagens |
description |
Feto-maternal communication in the dog involves the differentiation of stromal cells into decidual cells. As the only placental cells expressing the nuclear progesterone (P4) receptor (PGR), decidual cells play crucial roles in the maintenance and termination of pregnancy. Accordingly, to investigate possible PGR-mediated mechanisms in canine decidual cells, in vitro decidualized dog uterine stromal (DUS) cells were treated with functional PGR-blockers, mifepristone and aglepristone. Effects on decidualization markers, epithelial and mesenchymal factors, and markers of cellular viability were assessed. Decidualization increased the expression of PTGES, PGR, IGF1, and PRLR, along with ECM1, COL4 and CX43, but downregulated IGF2. DUS cells retained their mesenchymal character, and the expression of COL4 indicated the mesenchymal-epithelial transformation. Antigestagen treatment decreased the availability of PTGES, PRLR, IGF1 and PGR. Furthermore, antigestagens decreased the mRNA and protein expression of CX43, and transcriptional levels of ECM1 and COL4. Additionally, antigestagens increased levels of activated-CASP3 (a proapoptotic factor), associated with lowered levels of PCNA (a proliferation marker). These data reveal important aspects of the functional involvement of PGR in canine decidual cells, regarding the expression of decidualization markers and acquisition of epithelial-like characteristics. Some of these mechanisms may be crucial for the maintenance and/or termination of canine pregnancy. |
format |
Article in Journal/Newspaper |
author |
Kazemian, Ali Tavares Pereira, Miguel Hoffmann, Bernd Kowalewski, Mariusz P |
author_facet |
Kazemian, Ali Tavares Pereira, Miguel Hoffmann, Bernd Kowalewski, Mariusz P |
author_sort |
Kazemian, Ali |
title |
Antigestagens Mediate the Expression of Decidualization Markers, Extracellular Matrix Factors and Connexin 43 in Decidualized Dog Uterine Stromal (DUS) Cells |
title_short |
Antigestagens Mediate the Expression of Decidualization Markers, Extracellular Matrix Factors and Connexin 43 in Decidualized Dog Uterine Stromal (DUS) Cells |
title_full |
Antigestagens Mediate the Expression of Decidualization Markers, Extracellular Matrix Factors and Connexin 43 in Decidualized Dog Uterine Stromal (DUS) Cells |
title_fullStr |
Antigestagens Mediate the Expression of Decidualization Markers, Extracellular Matrix Factors and Connexin 43 in Decidualized Dog Uterine Stromal (DUS) Cells |
title_full_unstemmed |
Antigestagens Mediate the Expression of Decidualization Markers, Extracellular Matrix Factors and Connexin 43 in Decidualized Dog Uterine Stromal (DUS) Cells |
title_sort |
antigestagens mediate the expression of decidualization markers, extracellular matrix factors and connexin 43 in decidualized dog uterine stromal (dus) cells |
publisher |
MDPI Publishing |
publishDate |
2022 |
url |
https://www.zora.uzh.ch/id/eprint/223473/ https://www.zora.uzh.ch/id/eprint/223473/1/animals_12_00798.pdf https://doi.org/10.5167/uzh-223473 https://doi.org/10.3390/ani12070798 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Kazemian, Ali; Tavares Pereira, Miguel; Hoffmann, Bernd; Kowalewski, Mariusz P (2022). Antigestagens Mediate the Expression of Decidualization Markers, Extracellular Matrix Factors and Connexin 43 in Decidualized Dog Uterine Stromal (DUS) Cells. Animals, 12(7):798. |
op_relation |
https://www.zora.uzh.ch/id/eprint/223473/1/animals_12_00798.pdf doi:10.5167/uzh-223473 doi:10.3390/ani12070798 info:pmid/35405788 urn:issn:2076-2615 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons: Attribution 4.0 International (CC BY 4.0) http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.5167/uzh-22347310.3390/ani12070798 |
_version_ |
1810438487927685120 |