Prostaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors
Prostaglandins (PGs) are important regulators of the early corpus luteum (CL) in the dog. Whereas, initially, CL is gonadotropin independent, in the second half of its lifespan, hypophyseal support is required. The transition period is marked by decreased availability of PGs, in particular of PGE2....
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ftunivzuerich:oai:www.zora.uzh.ch:182354 2024-06-23T07:52:01+00:00 Prostaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors Tavares Pereira, Miguel Gram, Aykut Nowaczyk, Renata Boos, Alois Hoffmann, Bernd Janowski, Tomasz Kowalewski, Mariusz P 2019-03-01 application/pdf https://www.zora.uzh.ch/id/eprint/182354/ https://www.zora.uzh.ch/id/eprint/182354/1/1-s2.0-S1642431X18303991-main.pdf https://doi.org/10.5167/uzh-182354 https://doi.org/10.1016/j.repbio.2019.02.001 eng eng Elsevier https://www.zora.uzh.ch/id/eprint/182354/1/1-s2.0-S1642431X18303991-main.pdf doi:10.5167/uzh-182354 doi:10.1016/j.repbio.2019.02.001 info:pmid/30929911 urn:issn:1642-431X info:eu-repo/semantics/openAccess Creative Commons: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) http://creativecommons.org/licenses/by-nc-nd/4.0/ Tavares Pereira, Miguel; Gram, Aykut; Nowaczyk, Renata; Boos, Alois; Hoffmann, Bernd; Janowski, Tomasz; Kowalewski, Mariusz P (2019). Prostaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors. Reproductive Biology, 19(1):100-111. Institute of Veterinary Anatomy Center for Clinical Studies 570 Life sciences biology Developmental Biology Animal Science and Zoology Endocrinology Corpus luteum Dog (Canis lupus familiaris) Immune system Prostaglandins Vascularization Journal Article PeerReviewed info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2019 ftunivzuerich https://doi.org/10.5167/uzh-18235410.1016/j.repbio.2019.02.001 2024-06-12T00:36:08Z Prostaglandins (PGs) are important regulators of the early corpus luteum (CL) in the dog. Whereas, initially, CL is gonadotropin independent, in the second half of its lifespan, hypophyseal support is required. The transition period is marked by decreased availability of PGs, in particular of PGE2. We previously reported that inhibition of COX2/PTGS2 in vivo suppressed luteal production of PGE2, lowered circulating progesterone and negatively affected luteal development. Therefore, bitches were treated with a COX2-specific blocker, firocoxib, for 5, 10, 20 and 30 days after ovulation, leading to suppression of the steroidogenic machinery. Control groups received a placebo for the same periods. Considering the wide range of possible modulatory roles of PGs shown in different organ systems, this follow-up project aimed to understand further possible PG-mediated effects in early canine CL. Thirty-four (34) factors related predominantly to vascularization and immune response were screened (mRNAs and proteins) on samples from the above described in vivo study. Most of the effects were observed during the transitional period (days 20 and 30). The inhibition of COX2 diminished the expression of angiopoietin family members ANGPT1, -2, Tie1 and -2 receptors. The expression of endothelin (ET)-1 was increased. Concerning the immune system, increased expression of the pro-inflammatory cytokines, IL1β, IL6 and IL12a, and elevated expression levels of CD4, was observed. Cumulatively, besides its involvement in regulating steroidogenesis, our results indicate a broader role of PGs in the canine CL, including modulation of angiogenesis, vascular stabilization and local immunomodulation. Possible cross-species translational effects are strongly implied. Article in Journal/Newspaper Canis lupus University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
institution |
Open Polar |
collection |
University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
op_collection_id |
ftunivzuerich |
language |
English |
topic |
Institute of Veterinary Anatomy Center for Clinical Studies 570 Life sciences biology Developmental Biology Animal Science and Zoology Endocrinology Corpus luteum Dog (Canis lupus familiaris) Immune system Prostaglandins Vascularization |
spellingShingle |
Institute of Veterinary Anatomy Center for Clinical Studies 570 Life sciences biology Developmental Biology Animal Science and Zoology Endocrinology Corpus luteum Dog (Canis lupus familiaris) Immune system Prostaglandins Vascularization Tavares Pereira, Miguel Gram, Aykut Nowaczyk, Renata Boos, Alois Hoffmann, Bernd Janowski, Tomasz Kowalewski, Mariusz P Prostaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors |
topic_facet |
Institute of Veterinary Anatomy Center for Clinical Studies 570 Life sciences biology Developmental Biology Animal Science and Zoology Endocrinology Corpus luteum Dog (Canis lupus familiaris) Immune system Prostaglandins Vascularization |
description |
Prostaglandins (PGs) are important regulators of the early corpus luteum (CL) in the dog. Whereas, initially, CL is gonadotropin independent, in the second half of its lifespan, hypophyseal support is required. The transition period is marked by decreased availability of PGs, in particular of PGE2. We previously reported that inhibition of COX2/PTGS2 in vivo suppressed luteal production of PGE2, lowered circulating progesterone and negatively affected luteal development. Therefore, bitches were treated with a COX2-specific blocker, firocoxib, for 5, 10, 20 and 30 days after ovulation, leading to suppression of the steroidogenic machinery. Control groups received a placebo for the same periods. Considering the wide range of possible modulatory roles of PGs shown in different organ systems, this follow-up project aimed to understand further possible PG-mediated effects in early canine CL. Thirty-four (34) factors related predominantly to vascularization and immune response were screened (mRNAs and proteins) on samples from the above described in vivo study. Most of the effects were observed during the transitional period (days 20 and 30). The inhibition of COX2 diminished the expression of angiopoietin family members ANGPT1, -2, Tie1 and -2 receptors. The expression of endothelin (ET)-1 was increased. Concerning the immune system, increased expression of the pro-inflammatory cytokines, IL1β, IL6 and IL12a, and elevated expression levels of CD4, was observed. Cumulatively, besides its involvement in regulating steroidogenesis, our results indicate a broader role of PGs in the canine CL, including modulation of angiogenesis, vascular stabilization and local immunomodulation. Possible cross-species translational effects are strongly implied. |
format |
Article in Journal/Newspaper |
author |
Tavares Pereira, Miguel Gram, Aykut Nowaczyk, Renata Boos, Alois Hoffmann, Bernd Janowski, Tomasz Kowalewski, Mariusz P |
author_facet |
Tavares Pereira, Miguel Gram, Aykut Nowaczyk, Renata Boos, Alois Hoffmann, Bernd Janowski, Tomasz Kowalewski, Mariusz P |
author_sort |
Tavares Pereira, Miguel |
title |
Prostaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors |
title_short |
Prostaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors |
title_full |
Prostaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors |
title_fullStr |
Prostaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors |
title_full_unstemmed |
Prostaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors |
title_sort |
prostaglandin-mediated effects in early canine corpus luteum: in vivo effects on vascular and immune factors |
publisher |
Elsevier |
publishDate |
2019 |
url |
https://www.zora.uzh.ch/id/eprint/182354/ https://www.zora.uzh.ch/id/eprint/182354/1/1-s2.0-S1642431X18303991-main.pdf https://doi.org/10.5167/uzh-182354 https://doi.org/10.1016/j.repbio.2019.02.001 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Tavares Pereira, Miguel; Gram, Aykut; Nowaczyk, Renata; Boos, Alois; Hoffmann, Bernd; Janowski, Tomasz; Kowalewski, Mariusz P (2019). Prostaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors. Reproductive Biology, 19(1):100-111. |
op_relation |
https://www.zora.uzh.ch/id/eprint/182354/1/1-s2.0-S1642431X18303991-main.pdf doi:10.5167/uzh-182354 doi:10.1016/j.repbio.2019.02.001 info:pmid/30929911 urn:issn:1642-431X |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) http://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_doi |
https://doi.org/10.5167/uzh-18235410.1016/j.repbio.2019.02.001 |
_version_ |
1802643198347051008 |