Temporal and spatial variation in distribution of fish environmental DNA in England’s largest lake
Environmental DNA offers great potential as a biodiversity monitoring tool. Previous work has demonstrated that eDNA metabarcoding provides reliable information for lake fish monitoring, but important questions remain about temporal and spatial repeatability, which is critical for understanding the...
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2019
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ftunivzuerich:oai:www.zora.uzh.ch:175850 2024-06-23T07:48:55+00:00 Temporal and spatial variation in distribution of fish environmental DNA in England’s largest lake Lawson Handley, Lori Read, Daniel S Winfield, Ian J Kimbell, Helen Johnson, Harriet Li, Jianlong Hahn, Christoph Blackman, Rosetta Wilcox, Rose Donnelly, Rob Szitenberg, Amir Hänfling, Bernd 2019-05-01 application/pdf https://www.zora.uzh.ch/id/eprint/175850/ https://www.zora.uzh.ch/id/eprint/175850/1/Handley_et_al-2019-Environmental_DNA.pdf https://www.biorxiv.org/content/10.1101/376400v1 https://doi.org/10.5167/uzh-175850 https://doi.org/10.1002/edn3.5 eng eng Wiley-Blackwell Publishing, Inc. https://www.zora.uzh.ch/id/eprint/175850/1/Handley_et_al-2019-Environmental_DNA.pdf doi:10.5167/uzh-175850 doi:10.1002/edn3.5 urn:issn:2637-4943 info:eu-repo/semantics/openAccess Lawson Handley, Lori; Read, Daniel S; Winfield, Ian J; Kimbell, Helen; Johnson, Harriet; Li, Jianlong; Hahn, Christoph; Blackman, Rosetta; Wilcox, Rose; Donnelly, Rob; Szitenberg, Amir; Hänfling, Bernd (2019). Temporal and spatial variation in distribution of fish environmental DNA in England’s largest lake. Environmental DNA, 1(1):26-39. Institute of Evolutionary Biology and Environmental Studies 570 Life sciences biology 590 Animals (Zoology) eDNA fish lakes metabarcoding monitoring Journal Article NonPeerReviewed info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2019 ftunivzuerich https://doi.org/10.5167/uzh-17585010.1002/edn3.5 2024-06-05T00:23:47Z Environmental DNA offers great potential as a biodiversity monitoring tool. Previous work has demonstrated that eDNA metabarcoding provides reliable information for lake fish monitoring, but important questions remain about temporal and spatial repeatability, which is critical for understanding the ecology of eDNA and developing effective sampling strategies. Here, we carried out comprehensive spatial sampling of England's largest lake, Windermere, during summer and winter to (1) examine repeatability of the method, (2) compare eDNA results with contemporary gill‐net survey data, (3) test the hypothesis of greater spatial structure of eDNA in summer compared to winter due to differences in water mixing between seasons, and (4) compare the effectiveness of shore and offshore sampling for species detection. We find broad consistency between the results from three sampling events in terms of species detection and abundance, with eDNA detecting more species than established methods and being significantly correlated with rank abundance determined by long‐term data. As predicted, spatial structure was much greater in the summer, reflecting less mixing of eDNA than in the winter. For example Arctic charr, a deep-water species, was only detected in deep, midlake samples in the summer, while littoral or benthic species such as minnow and stickleback were more frequently detected in shore samples. By contrast in winter, the eDNA of these species was more uniformly distributed. This has important implications for design of sampling campaigns, for example, deep‐water species could be missed and littoral/benthic species overrepresented by focusing exclusively on shoreline samples collected in the summer. Article in Journal/Newspaper Arctic charr Arctic University of Zurich (UZH): ZORA (Zurich Open Repository and Archive Arctic |
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Open Polar |
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University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
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ftunivzuerich |
language |
English |
topic |
Institute of Evolutionary Biology and Environmental Studies 570 Life sciences biology 590 Animals (Zoology) eDNA fish lakes metabarcoding monitoring |
spellingShingle |
Institute of Evolutionary Biology and Environmental Studies 570 Life sciences biology 590 Animals (Zoology) eDNA fish lakes metabarcoding monitoring Lawson Handley, Lori Read, Daniel S Winfield, Ian J Kimbell, Helen Johnson, Harriet Li, Jianlong Hahn, Christoph Blackman, Rosetta Wilcox, Rose Donnelly, Rob Szitenberg, Amir Hänfling, Bernd Temporal and spatial variation in distribution of fish environmental DNA in England’s largest lake |
topic_facet |
Institute of Evolutionary Biology and Environmental Studies 570 Life sciences biology 590 Animals (Zoology) eDNA fish lakes metabarcoding monitoring |
description |
Environmental DNA offers great potential as a biodiversity monitoring tool. Previous work has demonstrated that eDNA metabarcoding provides reliable information for lake fish monitoring, but important questions remain about temporal and spatial repeatability, which is critical for understanding the ecology of eDNA and developing effective sampling strategies. Here, we carried out comprehensive spatial sampling of England's largest lake, Windermere, during summer and winter to (1) examine repeatability of the method, (2) compare eDNA results with contemporary gill‐net survey data, (3) test the hypothesis of greater spatial structure of eDNA in summer compared to winter due to differences in water mixing between seasons, and (4) compare the effectiveness of shore and offshore sampling for species detection. We find broad consistency between the results from three sampling events in terms of species detection and abundance, with eDNA detecting more species than established methods and being significantly correlated with rank abundance determined by long‐term data. As predicted, spatial structure was much greater in the summer, reflecting less mixing of eDNA than in the winter. For example Arctic charr, a deep-water species, was only detected in deep, midlake samples in the summer, while littoral or benthic species such as minnow and stickleback were more frequently detected in shore samples. By contrast in winter, the eDNA of these species was more uniformly distributed. This has important implications for design of sampling campaigns, for example, deep‐water species could be missed and littoral/benthic species overrepresented by focusing exclusively on shoreline samples collected in the summer. |
format |
Article in Journal/Newspaper |
author |
Lawson Handley, Lori Read, Daniel S Winfield, Ian J Kimbell, Helen Johnson, Harriet Li, Jianlong Hahn, Christoph Blackman, Rosetta Wilcox, Rose Donnelly, Rob Szitenberg, Amir Hänfling, Bernd |
author_facet |
Lawson Handley, Lori Read, Daniel S Winfield, Ian J Kimbell, Helen Johnson, Harriet Li, Jianlong Hahn, Christoph Blackman, Rosetta Wilcox, Rose Donnelly, Rob Szitenberg, Amir Hänfling, Bernd |
author_sort |
Lawson Handley, Lori |
title |
Temporal and spatial variation in distribution of fish environmental DNA in England’s largest lake |
title_short |
Temporal and spatial variation in distribution of fish environmental DNA in England’s largest lake |
title_full |
Temporal and spatial variation in distribution of fish environmental DNA in England’s largest lake |
title_fullStr |
Temporal and spatial variation in distribution of fish environmental DNA in England’s largest lake |
title_full_unstemmed |
Temporal and spatial variation in distribution of fish environmental DNA in England’s largest lake |
title_sort |
temporal and spatial variation in distribution of fish environmental dna in england’s largest lake |
publisher |
Wiley-Blackwell Publishing, Inc. |
publishDate |
2019 |
url |
https://www.zora.uzh.ch/id/eprint/175850/ https://www.zora.uzh.ch/id/eprint/175850/1/Handley_et_al-2019-Environmental_DNA.pdf https://www.biorxiv.org/content/10.1101/376400v1 https://doi.org/10.5167/uzh-175850 https://doi.org/10.1002/edn3.5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic charr Arctic |
genre_facet |
Arctic charr Arctic |
op_source |
Lawson Handley, Lori; Read, Daniel S; Winfield, Ian J; Kimbell, Helen; Johnson, Harriet; Li, Jianlong; Hahn, Christoph; Blackman, Rosetta; Wilcox, Rose; Donnelly, Rob; Szitenberg, Amir; Hänfling, Bernd (2019). Temporal and spatial variation in distribution of fish environmental DNA in England’s largest lake. Environmental DNA, 1(1):26-39. |
op_relation |
https://www.zora.uzh.ch/id/eprint/175850/1/Handley_et_al-2019-Environmental_DNA.pdf doi:10.5167/uzh-175850 doi:10.1002/edn3.5 urn:issn:2637-4943 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.5167/uzh-17585010.1002/edn3.5 |
_version_ |
1802639237652152320 |