Luteal and hypophyseal expression of the canine relaxin (RLN) system during pregnancy: Implications for luteotropic function
By acting through its receptors (RXFP1, RXFP2), relaxin (RLN) exerts species-specific effects during pregnancy; possible luteotropic effects through stimulation of prolactin (PRL) release have been suggested. In the domestic dog (Canis lupus familiaris) serum PRL increases in pregnant bitches shortl...
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ftunivzuerich:oai:www.zora.uzh.ch:164895 2024-06-23T07:52:00+00:00 Luteal and hypophyseal expression of the canine relaxin (RLN) system during pregnancy: Implications for luteotropic function Nowak, Marta Boos, Alois Kowalewski, Mariusz P Ambrósio, Carlos E Ambrósio, C E ( Carlos E ) 2018-01-24 application/pdf https://www.zora.uzh.ch/id/eprint/164895/ https://www.zora.uzh.ch/id/eprint/164895/1/pone.0191374.pdf https://doi.org/10.5167/uzh-164895 https://doi.org/10.1371/journal.pone.0191374 eng eng Public Library of Science (PLoS) https://www.zora.uzh.ch/id/eprint/164895/1/pone.0191374.pdf doi:10.5167/uzh-164895 doi:10.1371/journal.pone.0191374 urn:issn:1932-6203 info:eu-repo/semantics/openAccess Creative Commons: Attribution 4.0 International (CC BY 4.0) http://creativecommons.org/licenses/by/4.0/ Nowak, Marta; Boos, Alois; Kowalewski, Mariusz P (2018). Luteal and hypophyseal expression of the canine relaxin (RLN) system during pregnancy: Implications for luteotropic function. PLoS ONE, 13(1):e0191374. Institute of Veterinary Anatomy 570 Life sciences biology General Biochemistry Genetics and Molecular Biology General Agricultural and Biological Sciences General Medicine Journal Article PeerReviewed info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2018 ftunivzuerich https://doi.org/10.5167/uzh-16489510.1371/journal.pone.0191374 2024-06-05T00:22:15Z By acting through its receptors (RXFP1, RXFP2), relaxin (RLN) exerts species-specific effects during pregnancy; possible luteotropic effects through stimulation of prolactin (PRL) release have been suggested. In the domestic dog (Canis lupus familiaris) serum PRL increases in pregnant bitches shortly after RLN appears in the circulation, and a possible functional relationship between the RLN and the PRL systems in regulating progesterone secretion has been implied. Therefore, here (Study 1) the luteal expression and localization of the RLN system was investigated by immunohistochemistry using custom-made antibodies and semi-quantitative PCR, at selected time points during gestation: pre-implantation (d. 8-12), post-implantation (d. 18-25), mid-gestation (d. 35-40) and at normal and antigestagen-induced luteolysis. Further, (Study 2) hypophyseal expression of the RLN system and its spatial association with PRL was assessed. Luteal expression of RLN, but not of its receptors, was time-dependent: it increased significantly following implantation towards mid-gestation and decreased at prepartum. Antigestagen treatment resulted in downregulation of RLN and RXFP2. Whereas RLN was localized in steroidogenic cells, RXFP1 and RXFP2 also stained strongly in macrophages and vascular endothelial cells. The RLN system was detected in the canine adenohypophysis and was co-localized with PRL in hypophyseal lactotrophs. The intraluteal RLN seems to be involved in regulating the canine corpus luteum (CL) in a time-dependent manner. The presence of RLN family members in the adenohypophysis implies their possible involvement in regulating the availability of PRL and other pituitary hormones. Article in Journal/Newspaper Canis lupus University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
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Open Polar |
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University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
op_collection_id |
ftunivzuerich |
language |
English |
topic |
Institute of Veterinary Anatomy 570 Life sciences biology General Biochemistry Genetics and Molecular Biology General Agricultural and Biological Sciences General Medicine |
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Institute of Veterinary Anatomy 570 Life sciences biology General Biochemistry Genetics and Molecular Biology General Agricultural and Biological Sciences General Medicine Nowak, Marta Boos, Alois Kowalewski, Mariusz P Luteal and hypophyseal expression of the canine relaxin (RLN) system during pregnancy: Implications for luteotropic function |
topic_facet |
Institute of Veterinary Anatomy 570 Life sciences biology General Biochemistry Genetics and Molecular Biology General Agricultural and Biological Sciences General Medicine |
description |
By acting through its receptors (RXFP1, RXFP2), relaxin (RLN) exerts species-specific effects during pregnancy; possible luteotropic effects through stimulation of prolactin (PRL) release have been suggested. In the domestic dog (Canis lupus familiaris) serum PRL increases in pregnant bitches shortly after RLN appears in the circulation, and a possible functional relationship between the RLN and the PRL systems in regulating progesterone secretion has been implied. Therefore, here (Study 1) the luteal expression and localization of the RLN system was investigated by immunohistochemistry using custom-made antibodies and semi-quantitative PCR, at selected time points during gestation: pre-implantation (d. 8-12), post-implantation (d. 18-25), mid-gestation (d. 35-40) and at normal and antigestagen-induced luteolysis. Further, (Study 2) hypophyseal expression of the RLN system and its spatial association with PRL was assessed. Luteal expression of RLN, but not of its receptors, was time-dependent: it increased significantly following implantation towards mid-gestation and decreased at prepartum. Antigestagen treatment resulted in downregulation of RLN and RXFP2. Whereas RLN was localized in steroidogenic cells, RXFP1 and RXFP2 also stained strongly in macrophages and vascular endothelial cells. The RLN system was detected in the canine adenohypophysis and was co-localized with PRL in hypophyseal lactotrophs. The intraluteal RLN seems to be involved in regulating the canine corpus luteum (CL) in a time-dependent manner. The presence of RLN family members in the adenohypophysis implies their possible involvement in regulating the availability of PRL and other pituitary hormones. |
author2 |
Ambrósio, Carlos E Ambrósio, C E ( Carlos E ) |
format |
Article in Journal/Newspaper |
author |
Nowak, Marta Boos, Alois Kowalewski, Mariusz P |
author_facet |
Nowak, Marta Boos, Alois Kowalewski, Mariusz P |
author_sort |
Nowak, Marta |
title |
Luteal and hypophyseal expression of the canine relaxin (RLN) system during pregnancy: Implications for luteotropic function |
title_short |
Luteal and hypophyseal expression of the canine relaxin (RLN) system during pregnancy: Implications for luteotropic function |
title_full |
Luteal and hypophyseal expression of the canine relaxin (RLN) system during pregnancy: Implications for luteotropic function |
title_fullStr |
Luteal and hypophyseal expression of the canine relaxin (RLN) system during pregnancy: Implications for luteotropic function |
title_full_unstemmed |
Luteal and hypophyseal expression of the canine relaxin (RLN) system during pregnancy: Implications for luteotropic function |
title_sort |
luteal and hypophyseal expression of the canine relaxin (rln) system during pregnancy: implications for luteotropic function |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://www.zora.uzh.ch/id/eprint/164895/ https://www.zora.uzh.ch/id/eprint/164895/1/pone.0191374.pdf https://doi.org/10.5167/uzh-164895 https://doi.org/10.1371/journal.pone.0191374 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Nowak, Marta; Boos, Alois; Kowalewski, Mariusz P (2018). Luteal and hypophyseal expression of the canine relaxin (RLN) system during pregnancy: Implications for luteotropic function. PLoS ONE, 13(1):e0191374. |
op_relation |
https://www.zora.uzh.ch/id/eprint/164895/1/pone.0191374.pdf doi:10.5167/uzh-164895 doi:10.1371/journal.pone.0191374 urn:issn:1932-6203 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons: Attribution 4.0 International (CC BY 4.0) http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.5167/uzh-16489510.1371/journal.pone.0191374 |
_version_ |
1802643187895894016 |