Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog
For many years, modifications of the uterine extracellular matrix (ECM) during gestation have not been considered as critical for successful canine (Canis lupus familiaris) pregnancy. However, previous reports indicated an effect of free-floating blastocysts on the composition of the uterine ECM. He...
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BioScientifica Ltd.
2018
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Online Access: | https://www.zora.uzh.ch/id/eprint/164894/ https://www.zora.uzh.ch/id/eprint/164894/1/%5B17417899_-_Reproduction%5D_Uterine_and_placental_distribution_of_selected_extracellular_matrix_%28ECM%29_components_in_the_dog.pdf https://doi.org/10.5167/uzh-164894 https://doi.org/10.1530/rep-17-0761 |
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ftunivzuerich:oai:www.zora.uzh.ch:164894 2024-06-23T07:52:00+00:00 Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog Graubner, Felix R Boos, Alois Aslan, Selim Kücükaslan, Ibrahim Kowalewski, Mariusz P 2018-05-01 application/pdf https://www.zora.uzh.ch/id/eprint/164894/ https://www.zora.uzh.ch/id/eprint/164894/1/%5B17417899_-_Reproduction%5D_Uterine_and_placental_distribution_of_selected_extracellular_matrix_%28ECM%29_components_in_the_dog.pdf https://doi.org/10.5167/uzh-164894 https://doi.org/10.1530/rep-17-0761 eng eng BioScientifica Ltd. https://www.zora.uzh.ch/id/eprint/164894/1/%5B17417899_-_Reproduction%5D_Uterine_and_placental_distribution_of_selected_extracellular_matrix_%28ECM%29_components_in_the_dog.pdf doi:10.5167/uzh-164894 doi:10.1530/rep-17-0761 info:pmid/29439094 urn:issn:1470-1626 info:eu-repo/semantics/closedAccess Graubner, Felix R; Boos, Alois; Aslan, Selim; Kücükaslan, Ibrahim; Kowalewski, Mariusz P (2018). Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog. Reproduction, 155(5):403-421. Institute of Veterinary Anatomy 570 Life sciences biology Obstetrics and Gynaecology Cell Biology Embryology Endocrinology Reproductive Medicine Journal Article PeerReviewed info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2018 ftunivzuerich https://doi.org/10.5167/uzh-16489410.1530/rep-17-0761 2024-06-05T00:22:15Z For many years, modifications of the uterine extracellular matrix (ECM) during gestation have not been considered as critical for successful canine (Canis lupus familiaris) pregnancy. However, previous reports indicated an effect of free-floating blastocysts on the composition of the uterine ECM. Here, the expression of selected genes involved in structural functions, cell-to-cell communication and inhibition of matrix metalloproteinases were targeted utilizing qPCR and immunohistochemistry. We found that canine free-floating embryos affect gene expression of FN1, ECM1 and TIMP4 This seems to be associated with modulation of trophoblast invasion, and proliferative and adhesive functions of the uterus. Although not modulated at the beginning of pregnancy, the decrease of structural ECM components (i.e. COL1, -3, -4 and LAMA2) from pre-implantation toward post-implantation at placentation sites appears to be associated with softening of the tissue in preparation for trophoblast invasion. The further decrease of these components at placentation sites at the time of prepartum luteolysis seems to be associated with preparation for the release of fetal membranes. Reflecting a high degree of communication, intercellular cell adhesion molecules are induced following placentation (Cx26) or increase gradually toward prepartum luteolysis (Cx43). The spatio-temporal expression of TIMPs suggests their active involvement in modulating fetal invasiveness, and together with ECM1, they appear to protect deeper endometrial structures from trophoblast invasion. With this, the dog appears to be an interesting model for investigating placental functions in other species, e.g. in humans in which Placenta accreta appears to share several similarities with canine subinvolution of placental sites (SIPS). In summary, the canine uterine ECM is only moderately modified in early pregnancy, but undergoes vigorous reorganization processes in the uterus and placenta following implantation. Article in Journal/Newspaper Canis lupus University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
institution |
Open Polar |
collection |
University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
op_collection_id |
ftunivzuerich |
language |
English |
topic |
Institute of Veterinary Anatomy 570 Life sciences biology Obstetrics and Gynaecology Cell Biology Embryology Endocrinology Reproductive Medicine |
spellingShingle |
Institute of Veterinary Anatomy 570 Life sciences biology Obstetrics and Gynaecology Cell Biology Embryology Endocrinology Reproductive Medicine Graubner, Felix R Boos, Alois Aslan, Selim Kücükaslan, Ibrahim Kowalewski, Mariusz P Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog |
topic_facet |
Institute of Veterinary Anatomy 570 Life sciences biology Obstetrics and Gynaecology Cell Biology Embryology Endocrinology Reproductive Medicine |
description |
For many years, modifications of the uterine extracellular matrix (ECM) during gestation have not been considered as critical for successful canine (Canis lupus familiaris) pregnancy. However, previous reports indicated an effect of free-floating blastocysts on the composition of the uterine ECM. Here, the expression of selected genes involved in structural functions, cell-to-cell communication and inhibition of matrix metalloproteinases were targeted utilizing qPCR and immunohistochemistry. We found that canine free-floating embryos affect gene expression of FN1, ECM1 and TIMP4 This seems to be associated with modulation of trophoblast invasion, and proliferative and adhesive functions of the uterus. Although not modulated at the beginning of pregnancy, the decrease of structural ECM components (i.e. COL1, -3, -4 and LAMA2) from pre-implantation toward post-implantation at placentation sites appears to be associated with softening of the tissue in preparation for trophoblast invasion. The further decrease of these components at placentation sites at the time of prepartum luteolysis seems to be associated with preparation for the release of fetal membranes. Reflecting a high degree of communication, intercellular cell adhesion molecules are induced following placentation (Cx26) or increase gradually toward prepartum luteolysis (Cx43). The spatio-temporal expression of TIMPs suggests their active involvement in modulating fetal invasiveness, and together with ECM1, they appear to protect deeper endometrial structures from trophoblast invasion. With this, the dog appears to be an interesting model for investigating placental functions in other species, e.g. in humans in which Placenta accreta appears to share several similarities with canine subinvolution of placental sites (SIPS). In summary, the canine uterine ECM is only moderately modified in early pregnancy, but undergoes vigorous reorganization processes in the uterus and placenta following implantation. |
format |
Article in Journal/Newspaper |
author |
Graubner, Felix R Boos, Alois Aslan, Selim Kücükaslan, Ibrahim Kowalewski, Mariusz P |
author_facet |
Graubner, Felix R Boos, Alois Aslan, Selim Kücükaslan, Ibrahim Kowalewski, Mariusz P |
author_sort |
Graubner, Felix R |
title |
Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog |
title_short |
Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog |
title_full |
Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog |
title_fullStr |
Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog |
title_full_unstemmed |
Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog |
title_sort |
uterine and placental distribution of selected extracellular matrix (ecm) components in the dog |
publisher |
BioScientifica Ltd. |
publishDate |
2018 |
url |
https://www.zora.uzh.ch/id/eprint/164894/ https://www.zora.uzh.ch/id/eprint/164894/1/%5B17417899_-_Reproduction%5D_Uterine_and_placental_distribution_of_selected_extracellular_matrix_%28ECM%29_components_in_the_dog.pdf https://doi.org/10.5167/uzh-164894 https://doi.org/10.1530/rep-17-0761 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Graubner, Felix R; Boos, Alois; Aslan, Selim; Kücükaslan, Ibrahim; Kowalewski, Mariusz P (2018). Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog. Reproduction, 155(5):403-421. |
op_relation |
https://www.zora.uzh.ch/id/eprint/164894/1/%5B17417899_-_Reproduction%5D_Uterine_and_placental_distribution_of_selected_extracellular_matrix_%28ECM%29_components_in_the_dog.pdf doi:10.5167/uzh-164894 doi:10.1530/rep-17-0761 info:pmid/29439094 urn:issn:1470-1626 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.5167/uzh-16489410.1530/rep-17-0761 |
_version_ |
1802643190442885120 |