eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity
Because significant global changes are currently underway in the Arctic, creating a large-scale standardized database for Arctic marine biodiversity is particularly pressing. This study evaluates the potential of aquatic environmental DNA (eDNA) meta-barcoding to detect Arctic coastal biodiversity c...
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Online Access: | https://www.zora.uzh.ch/id/eprint/156830/ https://www.zora.uzh.ch/id/eprint/156830/1/Lacoursire_Roussel_et_al_2018_Ecology_and_Evolution.pdf https://doi.org/10.5167/uzh-156830 https://doi.org/10.1002/ece3.4213 |
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ftunivzuerich:oai:www.zora.uzh.ch:156830 2024-06-23T07:48:35+00:00 eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity Lacoursière-Roussel, Anaïs Howland, Kimberly Normandeau, Eric Grey, Erin K Archambault, Philippe Deiner, Kristy Lodge, David M Hernandez, Cecilia Leduc, Noémie Bernatchez, Louis 2018-08-16 application/pdf https://www.zora.uzh.ch/id/eprint/156830/ https://www.zora.uzh.ch/id/eprint/156830/1/Lacoursire_Roussel_et_al_2018_Ecology_and_Evolution.pdf https://doi.org/10.5167/uzh-156830 https://doi.org/10.1002/ece3.4213 eng eng Wiley Open Access https://www.zora.uzh.ch/id/eprint/156830/1/Lacoursire_Roussel_et_al_2018_Ecology_and_Evolution.pdf doi:10.5167/uzh-156830 doi:10.1002/ece3.4213 urn:issn:2045-7758 info:eu-repo/semantics/openAccess Creative Commons: Attribution 4.0 International (CC BY 4.0) http://creativecommons.org/licenses/by/4.0/ Lacoursière-Roussel, Anaïs; Howland, Kimberly; Normandeau, Eric; Grey, Erin K; Archambault, Philippe; Deiner, Kristy; Lodge, David M; Hernandez, Cecilia; Leduc, Noémie; Bernatchez, Louis (2018). eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity. Ecology and Evolution, 8(16):7763-7777. Institute of Evolutionary Biology and Environmental Studies 570 Life sciences biology 590 Animals (Zoology) arctic coastal biodiversity eDNA metabarcoding global changes invasion spatio-temporal distribution Journal Article PeerReviewed info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2018 ftunivzuerich https://doi.org/10.5167/uzh-15683010.1002/ece3.4213 2024-05-29T00:51:00Z Because significant global changes are currently underway in the Arctic, creating a large-scale standardized database for Arctic marine biodiversity is particularly pressing. This study evaluates the potential of aquatic environmental DNA (eDNA) meta-barcoding to detect Arctic coastal biodiversity changes and characterizes the local spatio-temporal distribution of eDNA in two locations. We extracted and amplified eDNA using two COI primer pairs from ~80 water samples that were collected across two Canadian Arctic ports, Churchill and Iqaluit based on optimized sampling and preservation methods for remote regions surveys. Results demonstrate that aquatic eDNA surveys have the potential to document large-scale Arctic biodiversity change by providing a rapid overview of coastal metazoan biodiversity, detecting nonindigenous species, and allowing sampling in both open water and under the ice cover by local northern-based communities. We show that DNA sequences of ~50% of known Canadian Arctic species and potential invaders are currently present in public data-bases. A similar proportion of operational taxonomic units was identified at the species level with eDNA metabarcoding, for a total of 181 species identified at both sites. Despite the cold and well-mixed coastal environment, species composition was vertically heterogeneous, in part due to river inflow in the estuarine ecosystem, and differed between the water column and tide pools. Thus, COI-based eDNA metabarcoding may quickly improve large-scale Arctic biomonitoring using eDNA, but we caution that aquatic eDNA sampling needs to be standardized over space and time to accurately evaluate community structure changes. Article in Journal/Newspaper Arctic Arctic biodiversity Arctic Iqaluit University of Zurich (UZH): ZORA (Zurich Open Repository and Archive Arctic |
institution |
Open Polar |
collection |
University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
op_collection_id |
ftunivzuerich |
language |
English |
topic |
Institute of Evolutionary Biology and Environmental Studies 570 Life sciences biology 590 Animals (Zoology) arctic coastal biodiversity eDNA metabarcoding global changes invasion spatio-temporal distribution |
spellingShingle |
Institute of Evolutionary Biology and Environmental Studies 570 Life sciences biology 590 Animals (Zoology) arctic coastal biodiversity eDNA metabarcoding global changes invasion spatio-temporal distribution Lacoursière-Roussel, Anaïs Howland, Kimberly Normandeau, Eric Grey, Erin K Archambault, Philippe Deiner, Kristy Lodge, David M Hernandez, Cecilia Leduc, Noémie Bernatchez, Louis eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity |
topic_facet |
Institute of Evolutionary Biology and Environmental Studies 570 Life sciences biology 590 Animals (Zoology) arctic coastal biodiversity eDNA metabarcoding global changes invasion spatio-temporal distribution |
description |
Because significant global changes are currently underway in the Arctic, creating a large-scale standardized database for Arctic marine biodiversity is particularly pressing. This study evaluates the potential of aquatic environmental DNA (eDNA) meta-barcoding to detect Arctic coastal biodiversity changes and characterizes the local spatio-temporal distribution of eDNA in two locations. We extracted and amplified eDNA using two COI primer pairs from ~80 water samples that were collected across two Canadian Arctic ports, Churchill and Iqaluit based on optimized sampling and preservation methods for remote regions surveys. Results demonstrate that aquatic eDNA surveys have the potential to document large-scale Arctic biodiversity change by providing a rapid overview of coastal metazoan biodiversity, detecting nonindigenous species, and allowing sampling in both open water and under the ice cover by local northern-based communities. We show that DNA sequences of ~50% of known Canadian Arctic species and potential invaders are currently present in public data-bases. A similar proportion of operational taxonomic units was identified at the species level with eDNA metabarcoding, for a total of 181 species identified at both sites. Despite the cold and well-mixed coastal environment, species composition was vertically heterogeneous, in part due to river inflow in the estuarine ecosystem, and differed between the water column and tide pools. Thus, COI-based eDNA metabarcoding may quickly improve large-scale Arctic biomonitoring using eDNA, but we caution that aquatic eDNA sampling needs to be standardized over space and time to accurately evaluate community structure changes. |
format |
Article in Journal/Newspaper |
author |
Lacoursière-Roussel, Anaïs Howland, Kimberly Normandeau, Eric Grey, Erin K Archambault, Philippe Deiner, Kristy Lodge, David M Hernandez, Cecilia Leduc, Noémie Bernatchez, Louis |
author_facet |
Lacoursière-Roussel, Anaïs Howland, Kimberly Normandeau, Eric Grey, Erin K Archambault, Philippe Deiner, Kristy Lodge, David M Hernandez, Cecilia Leduc, Noémie Bernatchez, Louis |
author_sort |
Lacoursière-Roussel, Anaïs |
title |
eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity |
title_short |
eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity |
title_full |
eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity |
title_fullStr |
eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity |
title_full_unstemmed |
eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity |
title_sort |
edna metabarcoding as a new surveillance approach for coastal arctic biodiversity |
publisher |
Wiley Open Access |
publishDate |
2018 |
url |
https://www.zora.uzh.ch/id/eprint/156830/ https://www.zora.uzh.ch/id/eprint/156830/1/Lacoursire_Roussel_et_al_2018_Ecology_and_Evolution.pdf https://doi.org/10.5167/uzh-156830 https://doi.org/10.1002/ece3.4213 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Arctic biodiversity Arctic Iqaluit |
genre_facet |
Arctic Arctic biodiversity Arctic Iqaluit |
op_source |
Lacoursière-Roussel, Anaïs; Howland, Kimberly; Normandeau, Eric; Grey, Erin K; Archambault, Philippe; Deiner, Kristy; Lodge, David M; Hernandez, Cecilia; Leduc, Noémie; Bernatchez, Louis (2018). eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity. Ecology and Evolution, 8(16):7763-7777. |
op_relation |
https://www.zora.uzh.ch/id/eprint/156830/1/Lacoursire_Roussel_et_al_2018_Ecology_and_Evolution.pdf doi:10.5167/uzh-156830 doi:10.1002/ece3.4213 urn:issn:2045-7758 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons: Attribution 4.0 International (CC BY 4.0) http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.5167/uzh-15683010.1002/ece3.4213 |
_version_ |
1802638944055066624 |