Uterine responses to early pre-attachment embryos in the domestic dog and comparisons with other domestic animal species
In the dog, there is no luteolysis in the absence of pregnancy. Thus, this species lacks any anti-luteolytic endocrine signal as found in other species that modulate uterine function during the critical period of pregnancy establishment. Nevertheless, in the dog an embryo-maternal communication must...
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Society for the Study of Reproduction
2017
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ftunivzuerich:oai:www.zora.uzh.ch:145671 2024-06-23T07:52:01+00:00 Uterine responses to early pre-attachment embryos in the domestic dog and comparisons with other domestic animal species Graubner, Felix R Gram, Aykut Kautz, Ewa Bauersachs, Stefan Aslan, Selim Agaoglu, Ali R Boos, Alois Kowalewski, Mariusz P 2017 application/pdf https://www.zora.uzh.ch/id/eprint/145671/ https://www.zora.uzh.ch/id/eprint/145671/1/iox063.pdf https://doi.org/10.5167/uzh-145671 https://doi.org/10.1093/biolre/iox063 eng eng Society for the Study of Reproduction https://www.zora.uzh.ch/id/eprint/145671/1/iox063.pdf doi:10.5167/uzh-145671 doi:10.1093/biolre/iox063 info:pmid/28651344 urn:issn:0006-3363 info:eu-repo/semantics/openAccess Creative Commons: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) http://creativecommons.org/licenses/by-nc/4.0/ Graubner, Felix R; Gram, Aykut; Kautz, Ewa; Bauersachs, Stefan; Aslan, Selim; Agaoglu, Ali R; Boos, Alois; Kowalewski, Mariusz P (2017). Uterine responses to early pre-attachment embryos in the domestic dog and comparisons with other domestic animal species. Biology of Reproduction, 97(2):197-216. Institute of Veterinary Anatomy Department of Farm Animals 570 Life sciences biology dog (Canis lupus familiaris) early pregnancy embryo-maternal communication Journal Article PeerReviewed info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2017 ftunivzuerich https://doi.org/10.5167/uzh-14567110.1093/biolre/iox063 2024-05-29T00:43:18Z In the dog, there is no luteolysis in the absence of pregnancy. Thus, this species lacks any anti-luteolytic endocrine signal as found in other species that modulate uterine function during the critical period of pregnancy establishment. Nevertheless, in the dog an embryo-maternal communication must occur in order to prevent rejection of embryos. Based on this hypothesis, we performed microarray analysis of canine uterine samples collected during pre-attachment phase (days 10-12) and in corresponding non-pregnant controls, in order to elucidate the embryo attachment signal. An additional goal was to identify differences in uterine responses to pre-attachment embryos between dogs and other mammalian species exhibiting different reproductive patterns with regard to luteolysis, implantation, and preparation for placentation. Therefore, the canine microarray data were compared with gene sets from pigs, cattle, horses, and humans. We found 412 genes differentially regulated between the two experimental groups. The functional terms most strongly enriched in response to pre-attachment embryos related to extracellular matrix function and remodeling, and to immune and inflammatory responses. Several candidate genes were validated by semi-quantitative PCR. When compared with other species, best matches were found with human and equine counterparts. Especially for the pig, the majority of overlapping genes showed opposite expression patterns. Interestingly, 1926 genes did not pair with any of the other gene sets. Using a microarray approach, we report the uterine changes in the dog driven by the presence of embryos and compare these results with datasets from other mammalian species, finding common-, contrary-, and exclusively canine-regulated genes. Article in Journal/Newspaper Canis lupus University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
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Open Polar |
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University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
op_collection_id |
ftunivzuerich |
language |
English |
topic |
Institute of Veterinary Anatomy Department of Farm Animals 570 Life sciences biology dog (Canis lupus familiaris) early pregnancy embryo-maternal communication |
spellingShingle |
Institute of Veterinary Anatomy Department of Farm Animals 570 Life sciences biology dog (Canis lupus familiaris) early pregnancy embryo-maternal communication Graubner, Felix R Gram, Aykut Kautz, Ewa Bauersachs, Stefan Aslan, Selim Agaoglu, Ali R Boos, Alois Kowalewski, Mariusz P Uterine responses to early pre-attachment embryos in the domestic dog and comparisons with other domestic animal species |
topic_facet |
Institute of Veterinary Anatomy Department of Farm Animals 570 Life sciences biology dog (Canis lupus familiaris) early pregnancy embryo-maternal communication |
description |
In the dog, there is no luteolysis in the absence of pregnancy. Thus, this species lacks any anti-luteolytic endocrine signal as found in other species that modulate uterine function during the critical period of pregnancy establishment. Nevertheless, in the dog an embryo-maternal communication must occur in order to prevent rejection of embryos. Based on this hypothesis, we performed microarray analysis of canine uterine samples collected during pre-attachment phase (days 10-12) and in corresponding non-pregnant controls, in order to elucidate the embryo attachment signal. An additional goal was to identify differences in uterine responses to pre-attachment embryos between dogs and other mammalian species exhibiting different reproductive patterns with regard to luteolysis, implantation, and preparation for placentation. Therefore, the canine microarray data were compared with gene sets from pigs, cattle, horses, and humans. We found 412 genes differentially regulated between the two experimental groups. The functional terms most strongly enriched in response to pre-attachment embryos related to extracellular matrix function and remodeling, and to immune and inflammatory responses. Several candidate genes were validated by semi-quantitative PCR. When compared with other species, best matches were found with human and equine counterparts. Especially for the pig, the majority of overlapping genes showed opposite expression patterns. Interestingly, 1926 genes did not pair with any of the other gene sets. Using a microarray approach, we report the uterine changes in the dog driven by the presence of embryos and compare these results with datasets from other mammalian species, finding common-, contrary-, and exclusively canine-regulated genes. |
format |
Article in Journal/Newspaper |
author |
Graubner, Felix R Gram, Aykut Kautz, Ewa Bauersachs, Stefan Aslan, Selim Agaoglu, Ali R Boos, Alois Kowalewski, Mariusz P |
author_facet |
Graubner, Felix R Gram, Aykut Kautz, Ewa Bauersachs, Stefan Aslan, Selim Agaoglu, Ali R Boos, Alois Kowalewski, Mariusz P |
author_sort |
Graubner, Felix R |
title |
Uterine responses to early pre-attachment embryos in the domestic dog and comparisons with other domestic animal species |
title_short |
Uterine responses to early pre-attachment embryos in the domestic dog and comparisons with other domestic animal species |
title_full |
Uterine responses to early pre-attachment embryos in the domestic dog and comparisons with other domestic animal species |
title_fullStr |
Uterine responses to early pre-attachment embryos in the domestic dog and comparisons with other domestic animal species |
title_full_unstemmed |
Uterine responses to early pre-attachment embryos in the domestic dog and comparisons with other domestic animal species |
title_sort |
uterine responses to early pre-attachment embryos in the domestic dog and comparisons with other domestic animal species |
publisher |
Society for the Study of Reproduction |
publishDate |
2017 |
url |
https://www.zora.uzh.ch/id/eprint/145671/ https://www.zora.uzh.ch/id/eprint/145671/1/iox063.pdf https://doi.org/10.5167/uzh-145671 https://doi.org/10.1093/biolre/iox063 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Graubner, Felix R; Gram, Aykut; Kautz, Ewa; Bauersachs, Stefan; Aslan, Selim; Agaoglu, Ali R; Boos, Alois; Kowalewski, Mariusz P (2017). Uterine responses to early pre-attachment embryos in the domestic dog and comparisons with other domestic animal species. Biology of Reproduction, 97(2):197-216. |
op_relation |
https://www.zora.uzh.ch/id/eprint/145671/1/iox063.pdf doi:10.5167/uzh-145671 doi:10.1093/biolre/iox063 info:pmid/28651344 urn:issn:0006-3363 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) http://creativecommons.org/licenses/by-nc/4.0/ |
op_doi |
https://doi.org/10.5167/uzh-14567110.1093/biolre/iox063 |
_version_ |
1802643192416305152 |