Molecular examinations of Babesia microti in rodents and rodent-attached ticks from urban and sylvatic habitats in Germany
Small mammals serve as reservoir hosts for tick-borne pathogens, especially for those which are not transmitted transovarially in ticks - such as Babesia microti. Molecular investigations on the prevalence of B. microti in wild small mammals and on attached ticks from differently structured areas ma...
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ftunivzuerich:oai:www.zora.uzh.ch:110449 2024-05-12T08:07:02+00:00 Molecular examinations of Babesia microti in rodents and rodent-attached ticks from urban and sylvatic habitats in Germany Obiegala, Anna Pfeffer, Martin Pfister, Kurt Karnath, Carolin Silaghi, Cornelia 2015 application/pdf https://www.zora.uzh.ch/id/eprint/110449/ https://www.zora.uzh.ch/id/eprint/110449/1/Obiegala%20et%20al.,%20Ticks%20and%20Tick-borne%20Diseases%20%282015%29.pdf https://doi.org/10.5167/uzh-110449 https://doi.org/10.1016/j.ttbdis.2015.03.005 eng eng Elsevier https://www.zora.uzh.ch/id/eprint/110449/1/Obiegala%20et%20al.,%20Ticks%20and%20Tick-borne%20Diseases%20%282015%29.pdf doi:10.5167/uzh-110449 doi:10.1016/j.ttbdis.2015.03.005 urn:issn:1877-959X info:eu-repo/semantics/openAccess Obiegala, Anna; Pfeffer, Martin; Pfister, Kurt; Karnath, Carolin; Silaghi, Cornelia (2015). Molecular examinations of Babesia microti in rodents and rodent-attached ticks from urban and sylvatic habitats in Germany. Ticks and Tick-borne Diseases, 6(4):445-449. Institute of Parasitology 570 Life sciences biology 610 Medicine & health 600 Technology Journal Article PeerReviewed info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion 2015 ftunivzuerich https://doi.org/10.5167/uzh-11044910.1016/j.ttbdis.2015.03.005 2024-04-17T15:23:53Z Small mammals serve as reservoir hosts for tick-borne pathogens, especially for those which are not transmitted transovarially in ticks - such as Babesia microti. Molecular investigations on the prevalence of B. microti in wild small mammals and on attached ticks from differently structured areas may provide information on the circulation of B. microti in different ecological niches. In 2012 and 2013, 622 rodents (396 Myodes glareolus, 178 Apodemus flavicollis, 36 Apodemus sylvaticus, 4 Apodemus agrarius, 7 Microtus arvalis, 1 Microtus agrestis) were captured from three differently structured habitats (urban, sylvatic, recultivated) in Germany. Attached ticks were collected from 449 small mammals (3250 Ixodes ricinus, 7 Ixodes trianguliceps, 133 Dermacentor reticulatus). A representative selection of a maximum of 5 ticks per developmental stage and species per 30 rodents of each species, location and year resulting in 965 ticks was further investigated. DNA was extracted from tick, blood and spleen samples, and tested by PCR for the partial 18S rRNA gene of B. microti with subsequent sequencing. The prevalence was significantly higher in rodents from the sylvatic site (4.6%) than in rodents captured at both other sites (0-0.6%) (χ2= 11.95; p= 0.00125). Body and spleen weight of infected M. glareolus from the sylvatic site were significantly higher compared to those from non-infected individuals from that site (p = 0.00288 and p = 0.00017, respectively). Babesia microti DNA was detected in 3 out of 965 attached ticks (0.3%; 95%CI: 0-1) from all sites, but they derived exclusively from rodents captured at the sylvatic site. At the same site, I. ricinus nymphs (7.7%; 95%CI: 1-25.3) were significantly more often infected than I. ricinus larvae (0%; 95%CI: 0-1.3)(χ2 = 26.72; p <0.0001). The majority of positive rodents was also found at that site. I. trianguliceps occurred exclusively and the majority of M. glareolus at that site. Thus, it may be assumed that the circulation of B. microti is more efficient where ... Article in Journal/Newspaper Microtus arvalis University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
institution |
Open Polar |
collection |
University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
op_collection_id |
ftunivzuerich |
language |
English |
topic |
Institute of Parasitology 570 Life sciences biology 610 Medicine & health 600 Technology |
spellingShingle |
Institute of Parasitology 570 Life sciences biology 610 Medicine & health 600 Technology Obiegala, Anna Pfeffer, Martin Pfister, Kurt Karnath, Carolin Silaghi, Cornelia Molecular examinations of Babesia microti in rodents and rodent-attached ticks from urban and sylvatic habitats in Germany |
topic_facet |
Institute of Parasitology 570 Life sciences biology 610 Medicine & health 600 Technology |
description |
Small mammals serve as reservoir hosts for tick-borne pathogens, especially for those which are not transmitted transovarially in ticks - such as Babesia microti. Molecular investigations on the prevalence of B. microti in wild small mammals and on attached ticks from differently structured areas may provide information on the circulation of B. microti in different ecological niches. In 2012 and 2013, 622 rodents (396 Myodes glareolus, 178 Apodemus flavicollis, 36 Apodemus sylvaticus, 4 Apodemus agrarius, 7 Microtus arvalis, 1 Microtus agrestis) were captured from three differently structured habitats (urban, sylvatic, recultivated) in Germany. Attached ticks were collected from 449 small mammals (3250 Ixodes ricinus, 7 Ixodes trianguliceps, 133 Dermacentor reticulatus). A representative selection of a maximum of 5 ticks per developmental stage and species per 30 rodents of each species, location and year resulting in 965 ticks was further investigated. DNA was extracted from tick, blood and spleen samples, and tested by PCR for the partial 18S rRNA gene of B. microti with subsequent sequencing. The prevalence was significantly higher in rodents from the sylvatic site (4.6%) than in rodents captured at both other sites (0-0.6%) (χ2= 11.95; p= 0.00125). Body and spleen weight of infected M. glareolus from the sylvatic site were significantly higher compared to those from non-infected individuals from that site (p = 0.00288 and p = 0.00017, respectively). Babesia microti DNA was detected in 3 out of 965 attached ticks (0.3%; 95%CI: 0-1) from all sites, but they derived exclusively from rodents captured at the sylvatic site. At the same site, I. ricinus nymphs (7.7%; 95%CI: 1-25.3) were significantly more often infected than I. ricinus larvae (0%; 95%CI: 0-1.3)(χ2 = 26.72; p <0.0001). The majority of positive rodents was also found at that site. I. trianguliceps occurred exclusively and the majority of M. glareolus at that site. Thus, it may be assumed that the circulation of B. microti is more efficient where ... |
format |
Article in Journal/Newspaper |
author |
Obiegala, Anna Pfeffer, Martin Pfister, Kurt Karnath, Carolin Silaghi, Cornelia |
author_facet |
Obiegala, Anna Pfeffer, Martin Pfister, Kurt Karnath, Carolin Silaghi, Cornelia |
author_sort |
Obiegala, Anna |
title |
Molecular examinations of Babesia microti in rodents and rodent-attached ticks from urban and sylvatic habitats in Germany |
title_short |
Molecular examinations of Babesia microti in rodents and rodent-attached ticks from urban and sylvatic habitats in Germany |
title_full |
Molecular examinations of Babesia microti in rodents and rodent-attached ticks from urban and sylvatic habitats in Germany |
title_fullStr |
Molecular examinations of Babesia microti in rodents and rodent-attached ticks from urban and sylvatic habitats in Germany |
title_full_unstemmed |
Molecular examinations of Babesia microti in rodents and rodent-attached ticks from urban and sylvatic habitats in Germany |
title_sort |
molecular examinations of babesia microti in rodents and rodent-attached ticks from urban and sylvatic habitats in germany |
publisher |
Elsevier |
publishDate |
2015 |
url |
https://www.zora.uzh.ch/id/eprint/110449/ https://www.zora.uzh.ch/id/eprint/110449/1/Obiegala%20et%20al.,%20Ticks%20and%20Tick-borne%20Diseases%20%282015%29.pdf https://doi.org/10.5167/uzh-110449 https://doi.org/10.1016/j.ttbdis.2015.03.005 |
genre |
Microtus arvalis |
genre_facet |
Microtus arvalis |
op_source |
Obiegala, Anna; Pfeffer, Martin; Pfister, Kurt; Karnath, Carolin; Silaghi, Cornelia (2015). Molecular examinations of Babesia microti in rodents and rodent-attached ticks from urban and sylvatic habitats in Germany. Ticks and Tick-borne Diseases, 6(4):445-449. |
op_relation |
https://www.zora.uzh.ch/id/eprint/110449/1/Obiegala%20et%20al.,%20Ticks%20and%20Tick-borne%20Diseases%20%282015%29.pdf doi:10.5167/uzh-110449 doi:10.1016/j.ttbdis.2015.03.005 urn:issn:1877-959X |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.5167/uzh-11044910.1016/j.ttbdis.2015.03.005 |
_version_ |
1798849602698346496 |