Biotic and abiotic factors shape the microbiota of wild-caught populations of the arbovirus vector Culicoides imicola
Biting midges of the genus Culicoides are known vectors of arboviruses affecting human and animal health. However, little is known about Culicoides imicola microbiota and its influence on this insect’s biology. In this study, the impact of biotic and abiotic factors on C. imicola microbiota was char...
Published in: | Insect Molecular Biology |
---|---|
Main Authors: | , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2018
|
Subjects: | |
Online Access: | http://zaguan.unizar.es/record/79692 https://doi.org/10.1111/imb.12526 |
id |
ftunivzaraaneto:oai:zaguan.unizar.es:79692 |
---|---|
record_format |
openpolar |
spelling |
ftunivzaraaneto:oai:zaguan.unizar.es:79692 2023-05-15T15:50:38+02:00 Biotic and abiotic factors shape the microbiota of wild-caught populations of the arbovirus vector Culicoides imicola Díaz-Sánchez, S. Hernández-Jarguín, A. Torina, A. Fernández de Mera, I.G. Estrada-Peña, A. Villar, M. La Russa, F. Blanda, V. Vicente, J. Caracappa, S. Gortazar, C. de la Fuente, J. 2018 application/pdf http://zaguan.unizar.es/record/79692 https://doi.org/10.1111/imb.12526 eng eng info:eu-repo/grantAgreement/EC/H2020/643476/EU/COllaborative Management Platform for detection and Analyses of (Re-)emerging and foodborne outbreaks in Europe/COMPARE This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 643476-COMPARE http://zaguan.unizar.es/record/79692 doi:10.1111/imb.12526 All rights reserved http://www.europeana.eu/rights/rr-f/ info:eu-repo/semantics/article info:eu-repo/semantics/submittedVersion 2018 ftunivzaraaneto https://doi.org/10.1111/imb.12526 2022-03-10T16:36:24Z Biting midges of the genus Culicoides are known vectors of arboviruses affecting human and animal health. However, little is known about Culicoides imicola microbiota and its influence on this insect’s biology. In this study, the impact of biotic and abiotic factors on C. imicola microbiota was characterized using shotgun-metagenomic sequencing of whole-body DNA samples. Wild-caught C. imicola adult nulliparous females were sampled in two locations from Sicily, Italy. The climatic variables of temperature and soil moisture from both localities were recorded together with potential host bloodmeal sources. Shared core microbiome among C. imicola populations included Pseudomonas, Escherichia, Halomonas, Candidatus Zinderia, Propionibacterium, and Schizosaccharomyces. Specific and unique taxa were also found in C. imicola from each location, highlighting similarities and differences in microbiome composition between the two populations. DNA and protein identification showed differences in host preferences between the two populations, with Homo sapiens and Canis lupus familiaris L. being the preferred bloodmeal source in both locations. A principal component analysis showed that the combined effect of host preferences (H. sapiens) and local soil moisture factors shape the microbiome composition of wild-caught populations of C. imicola. These results contribute to characterizing the role of the microbiome in insect adaptation and its utility in predicting geographic expansion of Culicoides species with potential implications for the control of vector-borne diseases. Article in Journal/Newspaper Canis lupus Digital Repository of University of Zaragoza (ZAGUAN) Insect Molecular Biology 27 6 847 861 |
institution |
Open Polar |
collection |
Digital Repository of University of Zaragoza (ZAGUAN) |
op_collection_id |
ftunivzaraaneto |
language |
English |
description |
Biting midges of the genus Culicoides are known vectors of arboviruses affecting human and animal health. However, little is known about Culicoides imicola microbiota and its influence on this insect’s biology. In this study, the impact of biotic and abiotic factors on C. imicola microbiota was characterized using shotgun-metagenomic sequencing of whole-body DNA samples. Wild-caught C. imicola adult nulliparous females were sampled in two locations from Sicily, Italy. The climatic variables of temperature and soil moisture from both localities were recorded together with potential host bloodmeal sources. Shared core microbiome among C. imicola populations included Pseudomonas, Escherichia, Halomonas, Candidatus Zinderia, Propionibacterium, and Schizosaccharomyces. Specific and unique taxa were also found in C. imicola from each location, highlighting similarities and differences in microbiome composition between the two populations. DNA and protein identification showed differences in host preferences between the two populations, with Homo sapiens and Canis lupus familiaris L. being the preferred bloodmeal source in both locations. A principal component analysis showed that the combined effect of host preferences (H. sapiens) and local soil moisture factors shape the microbiome composition of wild-caught populations of C. imicola. These results contribute to characterizing the role of the microbiome in insect adaptation and its utility in predicting geographic expansion of Culicoides species with potential implications for the control of vector-borne diseases. |
format |
Article in Journal/Newspaper |
author |
Díaz-Sánchez, S. Hernández-Jarguín, A. Torina, A. Fernández de Mera, I.G. Estrada-Peña, A. Villar, M. La Russa, F. Blanda, V. Vicente, J. Caracappa, S. Gortazar, C. de la Fuente, J. |
spellingShingle |
Díaz-Sánchez, S. Hernández-Jarguín, A. Torina, A. Fernández de Mera, I.G. Estrada-Peña, A. Villar, M. La Russa, F. Blanda, V. Vicente, J. Caracappa, S. Gortazar, C. de la Fuente, J. Biotic and abiotic factors shape the microbiota of wild-caught populations of the arbovirus vector Culicoides imicola |
author_facet |
Díaz-Sánchez, S. Hernández-Jarguín, A. Torina, A. Fernández de Mera, I.G. Estrada-Peña, A. Villar, M. La Russa, F. Blanda, V. Vicente, J. Caracappa, S. Gortazar, C. de la Fuente, J. |
author_sort |
Díaz-Sánchez, S. |
title |
Biotic and abiotic factors shape the microbiota of wild-caught populations of the arbovirus vector Culicoides imicola |
title_short |
Biotic and abiotic factors shape the microbiota of wild-caught populations of the arbovirus vector Culicoides imicola |
title_full |
Biotic and abiotic factors shape the microbiota of wild-caught populations of the arbovirus vector Culicoides imicola |
title_fullStr |
Biotic and abiotic factors shape the microbiota of wild-caught populations of the arbovirus vector Culicoides imicola |
title_full_unstemmed |
Biotic and abiotic factors shape the microbiota of wild-caught populations of the arbovirus vector Culicoides imicola |
title_sort |
biotic and abiotic factors shape the microbiota of wild-caught populations of the arbovirus vector culicoides imicola |
publishDate |
2018 |
url |
http://zaguan.unizar.es/record/79692 https://doi.org/10.1111/imb.12526 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
info:eu-repo/grantAgreement/EC/H2020/643476/EU/COllaborative Management Platform for detection and Analyses of (Re-)emerging and foodborne outbreaks in Europe/COMPARE This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 643476-COMPARE http://zaguan.unizar.es/record/79692 doi:10.1111/imb.12526 |
op_rights |
All rights reserved http://www.europeana.eu/rights/rr-f/ |
op_doi |
https://doi.org/10.1111/imb.12526 |
container_title |
Insect Molecular Biology |
container_volume |
27 |
container_issue |
6 |
container_start_page |
847 |
op_container_end_page |
861 |
_version_ |
1766385627647967232 |