Soil environmental DNA metabarcoding in low-biomass regions requires protocol optimization: a case study in Antarctica

• Published in: Antarctic Science
• Main Authors: Olmedo-Rojas, Pamela, Jeunen, Gert Jan, Lamare, Miles, Turnbull, Johanna, Terauds, Aleks, Gemmell, Neil, Fraser, Ceridwen I.
• Format: Text
• Language: unknown
• Published: Research Online 2023
• Subjects: 18S; biodiversity; biogeography; desert; eDNA; operational taxonomic unit; remote environments; Antarctic; Larsemann Hills; Antarc*; Antarctica
• Online Access: https://ro.uow.edu.au/test2021/8009; https://doi.org/10.1017/S0954102022000384

Environmental DNA is a powerful tool for monitoring biodiversity. Although environmental DNA surveys have successfully been implemented in various environments, protocol choice has been shown to affect results and inferences. Thus far, few method comparison studies for soil have been undertaken. Her...