Spatial variability in iron nutritional status of large diatoms in the Sea of Okhotsk with special reference to the Amur River discharge
The Sea of Okhotsk is known as one of the most biologically productive regions among the world's oceans, and its productivity is supported in part by the discharge of iron (Fe)-rich water from the Amur River. However, little is known about the effect of riverine-derived Fe input on the physiolo...
Published in: | Biogeosciences |
---|---|
Main Authors: | , , , , , , , |
Format: | Text |
Language: | unknown |
Published: |
Scholarship at UWindsor
2014
|
Subjects: | |
Online Access: | https://scholar.uwindsor.ca/glierpub/572 https://doi.org/10.5194/bg-11-2503-2014 https://scholar.uwindsor.ca/context/glierpub/article/1574/viewcontent/bg_11_2503_2014.pdf |
id |
ftunivwindsor:oai:scholar.uwindsor.ca:glierpub-1574 |
---|---|
record_format |
openpolar |
spelling |
ftunivwindsor:oai:scholar.uwindsor.ca:glierpub-1574 2023-12-10T09:53:18+01:00 Spatial variability in iron nutritional status of large diatoms in the Sea of Okhotsk with special reference to the Amur River discharge Suzuki, K. Hattori-Saito, A. Sekiguchi, Y. Nishioka, J. Shigemitsu, M. Isada, T. Liu, H. McKay, R. M.L. 2014-05-07T07:00:00Z application/pdf https://scholar.uwindsor.ca/glierpub/572 https://doi.org/10.5194/bg-11-2503-2014 https://scholar.uwindsor.ca/context/glierpub/article/1574/viewcontent/bg_11_2503_2014.pdf unknown Scholarship at UWindsor https://scholar.uwindsor.ca/glierpub/572 doi:10.5194/bg-11-2503-2014 https://scholar.uwindsor.ca/context/glierpub/article/1574/viewcontent/bg_11_2503_2014.pdf http://creativecommons.org/licenses/by/4.0/ Great Lakes Institute for Environmental Research Publications Biochemistry Biophysics and Structural Biology Biodiversity Biology Life Sciences Marine Biology text 2014 ftunivwindsor https://doi.org/10.5194/bg-11-2503-2014 2023-11-11T23:13:18Z The Sea of Okhotsk is known as one of the most biologically productive regions among the world's oceans, and its productivity is supported in part by the discharge of iron (Fe)-rich water from the Amur River. However, little is known about the effect of riverine-derived Fe input on the physiology of the large diatoms which often flourish in surface waters of the productive continental shelf region. We conducted diatom-specific immunochemical ferredoxin (Fd) and flavodoxin (Fld) assays in order to investigate the spatial variability of Fe nutritional status in the microplankton-sized (20-200 1/4m; hereafter micro-sized) diatoms. The Fd index, defined as the proportion of Fd to the sum of Fd plus Fld accumulations in the cells, was used to assess their Fe nutritional status. Additionally, active chlorophyll fluorescence measurements using pulse-amplitude-modulated (PAM) fluorometry were carried out to obtain the maximum photochemical quantum efficiency (Fv/Fm) of photosystem II for the total micro-sized phytoplankton assemblages including diatoms. During our observations in the summer of 2006, the micro-sized diatoms were relatively abundant (> 10 1/4g C L−1) in the neritic region, and formed a massive bloom in Sakhalin Bay near the mouth of the Amur River. Values of the Fd index andFv/Fm were high (>0.9 and >0.65, respectively) near the river mouth, indicating that Fe was sufficient for growth of the diatoms. However, in oceanic waters of the Sea of Okhotsk, the diatom Fd index declined as cellular Fld accumulation increased. These results suggest that there was a distinct gradient in Fe nutritional status in the micro-sized diatoms from near the Amur River mouth to open waters in the Sea of Okhotsk. A significant correlation between dissolved Fe (D-Fe) concentration and the Fd index was found in waters off Sakhalin Island, indicating that D-Fe was a key factor for the photophysiology of this diatom size class. In the vicinity of the Kuril Islands between the Sea of Okhotsk and the Pacific Ocean, ... Text Sakhalin University of Windsor, Ontario: Scholarship at UWindsor Okhotsk Pacific Biogeosciences 11 9 2503 2517 |
institution |
Open Polar |
collection |
University of Windsor, Ontario: Scholarship at UWindsor |
op_collection_id |
ftunivwindsor |
language |
unknown |
topic |
Biochemistry Biophysics and Structural Biology Biodiversity Biology Life Sciences Marine Biology |
spellingShingle |
Biochemistry Biophysics and Structural Biology Biodiversity Biology Life Sciences Marine Biology Suzuki, K. Hattori-Saito, A. Sekiguchi, Y. Nishioka, J. Shigemitsu, M. Isada, T. Liu, H. McKay, R. M.L. Spatial variability in iron nutritional status of large diatoms in the Sea of Okhotsk with special reference to the Amur River discharge |
topic_facet |
Biochemistry Biophysics and Structural Biology Biodiversity Biology Life Sciences Marine Biology |
description |
The Sea of Okhotsk is known as one of the most biologically productive regions among the world's oceans, and its productivity is supported in part by the discharge of iron (Fe)-rich water from the Amur River. However, little is known about the effect of riverine-derived Fe input on the physiology of the large diatoms which often flourish in surface waters of the productive continental shelf region. We conducted diatom-specific immunochemical ferredoxin (Fd) and flavodoxin (Fld) assays in order to investigate the spatial variability of Fe nutritional status in the microplankton-sized (20-200 1/4m; hereafter micro-sized) diatoms. The Fd index, defined as the proportion of Fd to the sum of Fd plus Fld accumulations in the cells, was used to assess their Fe nutritional status. Additionally, active chlorophyll fluorescence measurements using pulse-amplitude-modulated (PAM) fluorometry were carried out to obtain the maximum photochemical quantum efficiency (Fv/Fm) of photosystem II for the total micro-sized phytoplankton assemblages including diatoms. During our observations in the summer of 2006, the micro-sized diatoms were relatively abundant (> 10 1/4g C L−1) in the neritic region, and formed a massive bloom in Sakhalin Bay near the mouth of the Amur River. Values of the Fd index andFv/Fm were high (>0.9 and >0.65, respectively) near the river mouth, indicating that Fe was sufficient for growth of the diatoms. However, in oceanic waters of the Sea of Okhotsk, the diatom Fd index declined as cellular Fld accumulation increased. These results suggest that there was a distinct gradient in Fe nutritional status in the micro-sized diatoms from near the Amur River mouth to open waters in the Sea of Okhotsk. A significant correlation between dissolved Fe (D-Fe) concentration and the Fd index was found in waters off Sakhalin Island, indicating that D-Fe was a key factor for the photophysiology of this diatom size class. In the vicinity of the Kuril Islands between the Sea of Okhotsk and the Pacific Ocean, ... |
format |
Text |
author |
Suzuki, K. Hattori-Saito, A. Sekiguchi, Y. Nishioka, J. Shigemitsu, M. Isada, T. Liu, H. McKay, R. M.L. |
author_facet |
Suzuki, K. Hattori-Saito, A. Sekiguchi, Y. Nishioka, J. Shigemitsu, M. Isada, T. Liu, H. McKay, R. M.L. |
author_sort |
Suzuki, K. |
title |
Spatial variability in iron nutritional status of large diatoms in the Sea of Okhotsk with special reference to the Amur River discharge |
title_short |
Spatial variability in iron nutritional status of large diatoms in the Sea of Okhotsk with special reference to the Amur River discharge |
title_full |
Spatial variability in iron nutritional status of large diatoms in the Sea of Okhotsk with special reference to the Amur River discharge |
title_fullStr |
Spatial variability in iron nutritional status of large diatoms in the Sea of Okhotsk with special reference to the Amur River discharge |
title_full_unstemmed |
Spatial variability in iron nutritional status of large diatoms in the Sea of Okhotsk with special reference to the Amur River discharge |
title_sort |
spatial variability in iron nutritional status of large diatoms in the sea of okhotsk with special reference to the amur river discharge |
publisher |
Scholarship at UWindsor |
publishDate |
2014 |
url |
https://scholar.uwindsor.ca/glierpub/572 https://doi.org/10.5194/bg-11-2503-2014 https://scholar.uwindsor.ca/context/glierpub/article/1574/viewcontent/bg_11_2503_2014.pdf |
geographic |
Okhotsk Pacific |
geographic_facet |
Okhotsk Pacific |
genre |
Sakhalin |
genre_facet |
Sakhalin |
op_source |
Great Lakes Institute for Environmental Research Publications |
op_relation |
https://scholar.uwindsor.ca/glierpub/572 doi:10.5194/bg-11-2503-2014 https://scholar.uwindsor.ca/context/glierpub/article/1574/viewcontent/bg_11_2503_2014.pdf |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.5194/bg-11-2503-2014 |
container_title |
Biogeosciences |
container_volume |
11 |
container_issue |
9 |
container_start_page |
2503 |
op_container_end_page |
2517 |
_version_ |
1784899957417836544 |