A deep dive into fat: Investigating blubber lipidomic fingerprint of killer whales and humpback whales in northern Norway
In cetaceans, blubber is the primary and largest lipid body reservoir. Our current understanding about lipid stores and uses in cetaceans is still limited, and most studies only focused on a single narrow snapshot of the lipidome. We documented an extended lipidomic fingerprint in two cetacean speci...
Published in: | Ecology and Evolution |
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2021
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Online Access: | https://scholar.uwindsor.ca/glierpub/267 https://doi.org/10.1002/ece3.7523 https://scholar.uwindsor.ca/context/glierpub/article/1269/viewcontent/Ecology_and_Evolution___2021___Bories___A_deep_dive_into_fat__Investigating_blubber_lipidomic_fingerprint_of_killer_whales__1_.pdf |
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ftunivwindsor:oai:scholar.uwindsor.ca:glierpub-1269 2023-06-11T04:13:42+02:00 A deep dive into fat: Investigating blubber lipidomic fingerprint of killer whales and humpback whales in northern Norway Bories, Pierre Rikardsen, Audun H. Leonards, Pim Fisk, Aaron T. Tartu, Sabrina Vogel, Emma F. Bytingsvik, Jenny Blévin, Pierre 2021-06-01T07:00:00Z application/pdf https://scholar.uwindsor.ca/glierpub/267 https://doi.org/10.1002/ece3.7523 https://scholar.uwindsor.ca/context/glierpub/article/1269/viewcontent/Ecology_and_Evolution___2021___Bories___A_deep_dive_into_fat__Investigating_blubber_lipidomic_fingerprint_of_killer_whales__1_.pdf unknown Scholarship at UWindsor https://scholar.uwindsor.ca/glierpub/267 doi:10.1002/ece3.7523 https://scholar.uwindsor.ca/context/glierpub/article/1269/viewcontent/Ecology_and_Evolution___2021___Bories___A_deep_dive_into_fat__Investigating_blubber_lipidomic_fingerprint_of_killer_whales__1_.pdf http://creativecommons.org/licenses/by/4.0/ Great Lakes Institute for Environmental Research Publications capital breeder cetacean herring eater income breeder LION/web lipid seal eater stable isotope Biochemistry Biophysics and Structural Biology Biodiversity Biology Life Sciences Marine Biology text 2021 ftunivwindsor https://doi.org/10.1002/ece3.7523 2023-05-06T19:10:44Z In cetaceans, blubber is the primary and largest lipid body reservoir. Our current understanding about lipid stores and uses in cetaceans is still limited, and most studies only focused on a single narrow snapshot of the lipidome. We documented an extended lipidomic fingerprint in two cetacean species present in northern Norway during wintertime. We were able to detect 817 molecular lipid species in blubber of killer whales (Orcinus orca) and humpback whales (Megaptera novaeangliae). The profiles were largely dominated by triradylglycerols in both species and, to a lesser extent, by other constituents including glycerophosphocholines, phosphosphingolipids, glycerophosphoethanolamines, and diradylglycerols. Through a unique combination of traditional statistical approaches, together with a novel bioinformatic tool (LION/web), we showed contrasting fingerprint composition between species. The higher content of triradylglycerols in humpback whales is necessary to fuel their upcoming half a year fasting and energy-demanding migration between feeding and breeding grounds. In adipocytes, we assume that the intense feeding rate of humpback whales prior to migration translates into an important accumulation of triacylglycerol content in lipid droplets. Upstream, the endoplasmic reticulum is operating at full capacity to supply acute lipid storage, consistent with the reported enrichment of glycerophosphocholines in humpback whales, major components of the endoplasmic reticulum. There was also an enrichment of membrane components, which translates into higher sphingolipid content in the lipidome of killer whales, potentially as a structural adaptation for their higher hydrodynamic performance. Finally, the presence of both lipid-enriched and lipid-depleted individuals within the killer whale population in Norway suggests dietary specialization, consistent with significant differences in δ15N and δ13C isotopic ratios in skin between the two groups, with higher values and a wider niche for the lipid-enriched individuals. ... Text Killer Whale Megaptera novaeangliae Northern Norway Orca Orcinus orca Killer whale University of Windsor, Ontario: Scholarship at UWindsor Norway Ecology and Evolution 11 11 6716 6729 |
institution |
Open Polar |
collection |
University of Windsor, Ontario: Scholarship at UWindsor |
op_collection_id |
ftunivwindsor |
language |
unknown |
topic |
capital breeder cetacean herring eater income breeder LION/web lipid seal eater stable isotope Biochemistry Biophysics and Structural Biology Biodiversity Biology Life Sciences Marine Biology |
spellingShingle |
capital breeder cetacean herring eater income breeder LION/web lipid seal eater stable isotope Biochemistry Biophysics and Structural Biology Biodiversity Biology Life Sciences Marine Biology Bories, Pierre Rikardsen, Audun H. Leonards, Pim Fisk, Aaron T. Tartu, Sabrina Vogel, Emma F. Bytingsvik, Jenny Blévin, Pierre A deep dive into fat: Investigating blubber lipidomic fingerprint of killer whales and humpback whales in northern Norway |
topic_facet |
capital breeder cetacean herring eater income breeder LION/web lipid seal eater stable isotope Biochemistry Biophysics and Structural Biology Biodiversity Biology Life Sciences Marine Biology |
description |
In cetaceans, blubber is the primary and largest lipid body reservoir. Our current understanding about lipid stores and uses in cetaceans is still limited, and most studies only focused on a single narrow snapshot of the lipidome. We documented an extended lipidomic fingerprint in two cetacean species present in northern Norway during wintertime. We were able to detect 817 molecular lipid species in blubber of killer whales (Orcinus orca) and humpback whales (Megaptera novaeangliae). The profiles were largely dominated by triradylglycerols in both species and, to a lesser extent, by other constituents including glycerophosphocholines, phosphosphingolipids, glycerophosphoethanolamines, and diradylglycerols. Through a unique combination of traditional statistical approaches, together with a novel bioinformatic tool (LION/web), we showed contrasting fingerprint composition between species. The higher content of triradylglycerols in humpback whales is necessary to fuel their upcoming half a year fasting and energy-demanding migration between feeding and breeding grounds. In adipocytes, we assume that the intense feeding rate of humpback whales prior to migration translates into an important accumulation of triacylglycerol content in lipid droplets. Upstream, the endoplasmic reticulum is operating at full capacity to supply acute lipid storage, consistent with the reported enrichment of glycerophosphocholines in humpback whales, major components of the endoplasmic reticulum. There was also an enrichment of membrane components, which translates into higher sphingolipid content in the lipidome of killer whales, potentially as a structural adaptation for their higher hydrodynamic performance. Finally, the presence of both lipid-enriched and lipid-depleted individuals within the killer whale population in Norway suggests dietary specialization, consistent with significant differences in δ15N and δ13C isotopic ratios in skin between the two groups, with higher values and a wider niche for the lipid-enriched individuals. ... |
format |
Text |
author |
Bories, Pierre Rikardsen, Audun H. Leonards, Pim Fisk, Aaron T. Tartu, Sabrina Vogel, Emma F. Bytingsvik, Jenny Blévin, Pierre |
author_facet |
Bories, Pierre Rikardsen, Audun H. Leonards, Pim Fisk, Aaron T. Tartu, Sabrina Vogel, Emma F. Bytingsvik, Jenny Blévin, Pierre |
author_sort |
Bories, Pierre |
title |
A deep dive into fat: Investigating blubber lipidomic fingerprint of killer whales and humpback whales in northern Norway |
title_short |
A deep dive into fat: Investigating blubber lipidomic fingerprint of killer whales and humpback whales in northern Norway |
title_full |
A deep dive into fat: Investigating blubber lipidomic fingerprint of killer whales and humpback whales in northern Norway |
title_fullStr |
A deep dive into fat: Investigating blubber lipidomic fingerprint of killer whales and humpback whales in northern Norway |
title_full_unstemmed |
A deep dive into fat: Investigating blubber lipidomic fingerprint of killer whales and humpback whales in northern Norway |
title_sort |
deep dive into fat: investigating blubber lipidomic fingerprint of killer whales and humpback whales in northern norway |
publisher |
Scholarship at UWindsor |
publishDate |
2021 |
url |
https://scholar.uwindsor.ca/glierpub/267 https://doi.org/10.1002/ece3.7523 https://scholar.uwindsor.ca/context/glierpub/article/1269/viewcontent/Ecology_and_Evolution___2021___Bories___A_deep_dive_into_fat__Investigating_blubber_lipidomic_fingerprint_of_killer_whales__1_.pdf |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Killer Whale Megaptera novaeangliae Northern Norway Orca Orcinus orca Killer whale |
genre_facet |
Killer Whale Megaptera novaeangliae Northern Norway Orca Orcinus orca Killer whale |
op_source |
Great Lakes Institute for Environmental Research Publications |
op_relation |
https://scholar.uwindsor.ca/glierpub/267 doi:10.1002/ece3.7523 https://scholar.uwindsor.ca/context/glierpub/article/1269/viewcontent/Ecology_and_Evolution___2021___Bories___A_deep_dive_into_fat__Investigating_blubber_lipidomic_fingerprint_of_killer_whales__1_.pdf |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1002/ece3.7523 |
container_title |
Ecology and Evolution |
container_volume |
11 |
container_issue |
11 |
container_start_page |
6716 |
op_container_end_page |
6729 |
_version_ |
1768390963865059328 |