Standardization of fertilization protocols for the European eel, Anguilla anguilla
Standardization of artificial fertilization protocols for the European eel, Anguilla anguilla, is a prerequisite for optimizing the use of available gametes in hatchery facilities and for conserving sperm from high quality males, which is either cryopreserved or in living gene banks. The objectives...
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ftunivwindsor:oai:scholar.uwindsor.ca:glierpub-1103 2023-06-11T04:03:45+02:00 Standardization of fertilization protocols for the European eel, Anguilla anguilla Butts, Ian A.E. Sorensen, Sune R. Politis, Sebastian N. Pitcher, Trevor E. Tomkiewicz, Jonna 2014-01-01T08:00:00Z https://scholar.uwindsor.ca/glierpub/102 https://doi.org/10.1016/j.aquaculture.2014.01.020 unknown Scholarship at UWindsor https://scholar.uwindsor.ca/glierpub/102 doi:10.1016/j.aquaculture.2014.01.020 http://dx.doi.org/10.1016/j.aquaculture.2014.01.020 Great Lakes Institute for Environmental Research Publications Sperm density Spectrophotometer Sperm to egg ratio Fertilization success Gamete longevity Artificial fertilization Biochemistry Biophysics and Structural Biology Physical Sciences and Mathematics text 2014 ftunivwindsor https://doi.org/10.1016/j.aquaculture.2014.01.020 2023-05-06T19:04:13Z Standardization of artificial fertilization protocols for the European eel, Anguilla anguilla, is a prerequisite for optimizing the use of available gametes in hatchery facilities and for conserving sperm from high quality males, which is either cryopreserved or in living gene banks. The objectives of this research were to provide a rapid, accurate and precise method to quantify sperm density by examining the relationship between sperm density and absorbance by use of a spectrophotometer, determine the optimal number of sperm required to fertilize eggs in a controlled setting, and explore how long eggs are receptive to fertilization post-stripping. Mean sperm density and absorbance at 350 nm were 1.54e + 10 +/- 4.95e + 9 sperm/mL and 1.91 +/- 0.22 nm, respectively. Regression analysis demonstrated a highly significant positive relationship between sperm density and absorbance using a spectrophotometer at 350 nm(R-2 - 0.94, p < 0.001, y - 2.273e + 10x - 2.805e + 10); significant but slightly weaker relationships were also detected at 400, 500, and 600 nm (R-2 >= 0.93, p < 0.001). Fertilization success using sperm to egg ratios ranging from 1.3e + 3 to 1.0e + 6 sperm per egg increased from 37.5 to 68.1%, respectively. Spermto egg ratio had a significant effect on fertilization success (p b 0.0001), where fertilization success increased from1.3e + 3 to 2.5e + 4 sperm per egg; adding greater than 2.5e + 4 spermper egg had no significant effect. Furthermore, the duration of time post-stripping had a significant effect on egg fertilization success (p < 0.0001), such that between 0 and 10 min post-stripping 57.4 to 78.2% of the eggs were fertilized while at 15 min post-stripping a significant decrease in fertilization success was detected (47.5%). For all statistical models, the female variance component was significant for fertilization success (p < 0.0001) and explained >= 84% of the models variance. In conclusion, European eel eggs should be fertilized within 10 min post- stripping using 2.5e + 4 ... Text Anguilla anguilla University of Windsor, Ontario: Scholarship at UWindsor Aquaculture 426-427 9 13 |
institution |
Open Polar |
collection |
University of Windsor, Ontario: Scholarship at UWindsor |
op_collection_id |
ftunivwindsor |
language |
unknown |
topic |
Sperm density Spectrophotometer Sperm to egg ratio Fertilization success Gamete longevity Artificial fertilization Biochemistry Biophysics and Structural Biology Physical Sciences and Mathematics |
spellingShingle |
Sperm density Spectrophotometer Sperm to egg ratio Fertilization success Gamete longevity Artificial fertilization Biochemistry Biophysics and Structural Biology Physical Sciences and Mathematics Butts, Ian A.E. Sorensen, Sune R. Politis, Sebastian N. Pitcher, Trevor E. Tomkiewicz, Jonna Standardization of fertilization protocols for the European eel, Anguilla anguilla |
topic_facet |
Sperm density Spectrophotometer Sperm to egg ratio Fertilization success Gamete longevity Artificial fertilization Biochemistry Biophysics and Structural Biology Physical Sciences and Mathematics |
description |
Standardization of artificial fertilization protocols for the European eel, Anguilla anguilla, is a prerequisite for optimizing the use of available gametes in hatchery facilities and for conserving sperm from high quality males, which is either cryopreserved or in living gene banks. The objectives of this research were to provide a rapid, accurate and precise method to quantify sperm density by examining the relationship between sperm density and absorbance by use of a spectrophotometer, determine the optimal number of sperm required to fertilize eggs in a controlled setting, and explore how long eggs are receptive to fertilization post-stripping. Mean sperm density and absorbance at 350 nm were 1.54e + 10 +/- 4.95e + 9 sperm/mL and 1.91 +/- 0.22 nm, respectively. Regression analysis demonstrated a highly significant positive relationship between sperm density and absorbance using a spectrophotometer at 350 nm(R-2 - 0.94, p < 0.001, y - 2.273e + 10x - 2.805e + 10); significant but slightly weaker relationships were also detected at 400, 500, and 600 nm (R-2 >= 0.93, p < 0.001). Fertilization success using sperm to egg ratios ranging from 1.3e + 3 to 1.0e + 6 sperm per egg increased from 37.5 to 68.1%, respectively. Spermto egg ratio had a significant effect on fertilization success (p b 0.0001), where fertilization success increased from1.3e + 3 to 2.5e + 4 sperm per egg; adding greater than 2.5e + 4 spermper egg had no significant effect. Furthermore, the duration of time post-stripping had a significant effect on egg fertilization success (p < 0.0001), such that between 0 and 10 min post-stripping 57.4 to 78.2% of the eggs were fertilized while at 15 min post-stripping a significant decrease in fertilization success was detected (47.5%). For all statistical models, the female variance component was significant for fertilization success (p < 0.0001) and explained >= 84% of the models variance. In conclusion, European eel eggs should be fertilized within 10 min post- stripping using 2.5e + 4 ... |
format |
Text |
author |
Butts, Ian A.E. Sorensen, Sune R. Politis, Sebastian N. Pitcher, Trevor E. Tomkiewicz, Jonna |
author_facet |
Butts, Ian A.E. Sorensen, Sune R. Politis, Sebastian N. Pitcher, Trevor E. Tomkiewicz, Jonna |
author_sort |
Butts, Ian A.E. |
title |
Standardization of fertilization protocols for the European eel, Anguilla anguilla |
title_short |
Standardization of fertilization protocols for the European eel, Anguilla anguilla |
title_full |
Standardization of fertilization protocols for the European eel, Anguilla anguilla |
title_fullStr |
Standardization of fertilization protocols for the European eel, Anguilla anguilla |
title_full_unstemmed |
Standardization of fertilization protocols for the European eel, Anguilla anguilla |
title_sort |
standardization of fertilization protocols for the european eel, anguilla anguilla |
publisher |
Scholarship at UWindsor |
publishDate |
2014 |
url |
https://scholar.uwindsor.ca/glierpub/102 https://doi.org/10.1016/j.aquaculture.2014.01.020 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
Great Lakes Institute for Environmental Research Publications |
op_relation |
https://scholar.uwindsor.ca/glierpub/102 doi:10.1016/j.aquaculture.2014.01.020 http://dx.doi.org/10.1016/j.aquaculture.2014.01.020 |
op_doi |
https://doi.org/10.1016/j.aquaculture.2014.01.020 |
container_title |
Aquaculture |
container_volume |
426-427 |
container_start_page |
9 |
op_container_end_page |
13 |
_version_ |
1768382787505618944 |