Automated sperm head morphology analyzer for open-source software

Sperm head morphology has been identified as a characteristic that can be used to predict a male's semen quality. In the present study, we have developed an automated sperm head morphology analysis (ASMA) plug-in for open-source ImageJ software (http://rsbweb.nih.gov/ij/). We describe the plug-...

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Bibliographic Details
Published in:Theriogenology
Main Authors: Butts, I. A. E., Ward, M. A.R., Litvak, M. K., Pitcher, Trevor E., Alavi, S. M. H.
Format: Text
Language:unknown
Published: Scholarship at UWindsor 2011
Subjects:
Sperm morphology
Sperm head
Automated Sperm Morphology Analysis
Sperm competition
Atlantic cod
Reproductive biology
Biochemistry
Biophysics
and Structural Biology
Physical Sciences and Mathematics
atlantic cod
Online Access:https://scholar.uwindsor.ca/glierpub/93
https://doi.org/10.1016/j.theriogenology.2011.06.019
Description
Summary:Sperm head morphology has been identified as a characteristic that can be used to predict a male's semen quality. In the present study, we have developed an automated sperm head morphology analysis (ASMA) plug-in for open-source ImageJ software (http://rsbweb.nih.gov/ij/). We describe the plug-in's functionality, and confirm its validity for sperm head morphology analysis using fish sperm. Sperm head morphological measurements (length and width) made with the ASMA plug-in did not differ from manual measurements. Using the plug-in to measure sperm head-shaped objects of known size, the associated plug-in error rate was < 0.5%. Brightness and contrast ratios influenced sperm head measurements, suggesting the need for standardized protocols. This plug-in was effective at measuring elliptical (i.e., Atlantic cod) as well as slightly irregular (i.e., Chinook salmon) shaped sperm heads. In conclusion, our ASMA plug-in represents a versatile alternative to costly sperm morphology software. (C) 2011 Elsevier Inc. All rights reserved.

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