"Antarctic yeasts as a source of L-asparaginase: Characterization of a glutaminase-activity free L-asparaginase from psychrotolerant yeast Leucosporidium scottii L115"
"Microorganisms from extreme environments, such as the Antarctic ecosystems, have a great potential to produce enzymes with novel characteristics. Within this context, L-asparaginase (ASNase) obtained from yeast species has been poorly studied. In this study, yeasts isolated from samples collec...
Published in: | Process Biochemistry |
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Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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Elsevier Ltd
2023
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Online Access: | https://hdl.handle.net/20.500.13053/8612 https://doi.org/10.1016/j.procbio.2023.03.003 |
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ftunivwiener:oai:repositorio.uwiener.edu.pe:20.500.13053/8612 2023-10-09T21:47:11+02:00 "Antarctic yeasts as a source of L-asparaginase: Characterization of a glutaminase-activity free L-asparaginase from psychrotolerant yeast Leucosporidium scottii L115" Sanchez-Moguel, Ignacio Costa-Silva, Tales A. Pillaca-Pullo, Omar S. Flores-Santos, Juan Carlos Barros Freire, Rominne Karla Carretero, Gustavo Bueno, Júlia da Luz Camacho-Cordova, David I. Santos, Joáo H.P.M. Sette, Lara Duraes Pessoa-Jr., Adalberto 2023-03-08 application/pdf https://hdl.handle.net/20.500.13053/8612 https://doi.org/10.1016/j.procbio.2023.03.003 eng eng Elsevier Ltd CH https://hdl.handle.net/20.500.13053/8612 https://doi.org/10.1016/j.procbio.2023.03.003 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ "L-asparaginase Psychrotolerant yeast Leucosporidium scottii Antarctic ecosystems Cold-adapted yeast Leukemia" 3.00.00 -- Ciencias médicas Ciencias de la salud info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2023 ftunivwiener https://doi.org/20.500.13053/861210.1016/j.procbio.2023.03.003 2023-09-18T09:05:40Z "Microorganisms from extreme environments, such as the Antarctic ecosystems, have a great potential to produce enzymes with novel characteristics. Within this context, L-asparaginase (ASNase) obtained from yeast species has been poorly studied. In this study, yeasts isolated from samples collected at Admiralty Bay (King George Island, Antarctica) were tested to produce ASNase. From an initial screening of 40 strains, belonging to 13 different species, Leucosporidium scottii L115 produced an ASNase activity (LsASNase activity: 6.24 U g-1 of dry cell weight) with the lowest glutaminase activity. The LsASNase was purified 227-fold, with a specific activity of 137.01 U mg-1 at 37 ◦C, without glutaminase activity. Moreover, the maximum enzyme activity was observed at pH 7.5 and at a temperature of 55 ◦C. The enzyme is a multimer of 462 kDa, presenting a single band of 53 kDa molecular mass in reduced conditions; after PGNase F treatment, a single band of 45 kDa was observed. The enzymatic kinetic evaluation revealed an allosteric regulation of the enzyme and the kinetic parameters were determined at 37 ◦C, pH 7.0 as substrate affinity constant, K0.5 = 233 μM, kcat = 54.7 s − 1 and Hill coefficient, nH = 1.52, demonstrating positive cooperativity by the enzyme and the substrate. This is the first study to report L. scottii as a source of glutaminase-activity free L-asparaginase, an acute lymphoblastic leukemia drug feature suitable for the treatment of asparagine synthetase negative cancer cells." Article in Journal/Newspaper Antarc* Antarctic Antarctica King George Island Repositorial Digital Universidad Wiener - DSpace Principal Uwiener Antarctic The Antarctic King George Island Admiralty Bay Process Biochemistry 129 121 132 |
institution |
Open Polar |
collection |
Repositorial Digital Universidad Wiener - DSpace Principal Uwiener |
op_collection_id |
ftunivwiener |
language |
English |
topic |
"L-asparaginase Psychrotolerant yeast Leucosporidium scottii Antarctic ecosystems Cold-adapted yeast Leukemia" 3.00.00 -- Ciencias médicas Ciencias de la salud |
spellingShingle |
"L-asparaginase Psychrotolerant yeast Leucosporidium scottii Antarctic ecosystems Cold-adapted yeast Leukemia" 3.00.00 -- Ciencias médicas Ciencias de la salud Sanchez-Moguel, Ignacio Costa-Silva, Tales A. Pillaca-Pullo, Omar S. Flores-Santos, Juan Carlos Barros Freire, Rominne Karla Carretero, Gustavo Bueno, Júlia da Luz Camacho-Cordova, David I. Santos, Joáo H.P.M. Sette, Lara Duraes Pessoa-Jr., Adalberto "Antarctic yeasts as a source of L-asparaginase: Characterization of a glutaminase-activity free L-asparaginase from psychrotolerant yeast Leucosporidium scottii L115" |
topic_facet |
"L-asparaginase Psychrotolerant yeast Leucosporidium scottii Antarctic ecosystems Cold-adapted yeast Leukemia" 3.00.00 -- Ciencias médicas Ciencias de la salud |
description |
"Microorganisms from extreme environments, such as the Antarctic ecosystems, have a great potential to produce enzymes with novel characteristics. Within this context, L-asparaginase (ASNase) obtained from yeast species has been poorly studied. In this study, yeasts isolated from samples collected at Admiralty Bay (King George Island, Antarctica) were tested to produce ASNase. From an initial screening of 40 strains, belonging to 13 different species, Leucosporidium scottii L115 produced an ASNase activity (LsASNase activity: 6.24 U g-1 of dry cell weight) with the lowest glutaminase activity. The LsASNase was purified 227-fold, with a specific activity of 137.01 U mg-1 at 37 ◦C, without glutaminase activity. Moreover, the maximum enzyme activity was observed at pH 7.5 and at a temperature of 55 ◦C. The enzyme is a multimer of 462 kDa, presenting a single band of 53 kDa molecular mass in reduced conditions; after PGNase F treatment, a single band of 45 kDa was observed. The enzymatic kinetic evaluation revealed an allosteric regulation of the enzyme and the kinetic parameters were determined at 37 ◦C, pH 7.0 as substrate affinity constant, K0.5 = 233 μM, kcat = 54.7 s − 1 and Hill coefficient, nH = 1.52, demonstrating positive cooperativity by the enzyme and the substrate. This is the first study to report L. scottii as a source of glutaminase-activity free L-asparaginase, an acute lymphoblastic leukemia drug feature suitable for the treatment of asparagine synthetase negative cancer cells." |
format |
Article in Journal/Newspaper |
author |
Sanchez-Moguel, Ignacio Costa-Silva, Tales A. Pillaca-Pullo, Omar S. Flores-Santos, Juan Carlos Barros Freire, Rominne Karla Carretero, Gustavo Bueno, Júlia da Luz Camacho-Cordova, David I. Santos, Joáo H.P.M. Sette, Lara Duraes Pessoa-Jr., Adalberto |
author_facet |
Sanchez-Moguel, Ignacio Costa-Silva, Tales A. Pillaca-Pullo, Omar S. Flores-Santos, Juan Carlos Barros Freire, Rominne Karla Carretero, Gustavo Bueno, Júlia da Luz Camacho-Cordova, David I. Santos, Joáo H.P.M. Sette, Lara Duraes Pessoa-Jr., Adalberto |
author_sort |
Sanchez-Moguel, Ignacio |
title |
"Antarctic yeasts as a source of L-asparaginase: Characterization of a glutaminase-activity free L-asparaginase from psychrotolerant yeast Leucosporidium scottii L115" |
title_short |
"Antarctic yeasts as a source of L-asparaginase: Characterization of a glutaminase-activity free L-asparaginase from psychrotolerant yeast Leucosporidium scottii L115" |
title_full |
"Antarctic yeasts as a source of L-asparaginase: Characterization of a glutaminase-activity free L-asparaginase from psychrotolerant yeast Leucosporidium scottii L115" |
title_fullStr |
"Antarctic yeasts as a source of L-asparaginase: Characterization of a glutaminase-activity free L-asparaginase from psychrotolerant yeast Leucosporidium scottii L115" |
title_full_unstemmed |
"Antarctic yeasts as a source of L-asparaginase: Characterization of a glutaminase-activity free L-asparaginase from psychrotolerant yeast Leucosporidium scottii L115" |
title_sort |
"antarctic yeasts as a source of l-asparaginase: characterization of a glutaminase-activity free l-asparaginase from psychrotolerant yeast leucosporidium scottii l115" |
publisher |
Elsevier Ltd |
publishDate |
2023 |
url |
https://hdl.handle.net/20.500.13053/8612 https://doi.org/10.1016/j.procbio.2023.03.003 |
geographic |
Antarctic The Antarctic King George Island Admiralty Bay |
geographic_facet |
Antarctic The Antarctic King George Island Admiralty Bay |
genre |
Antarc* Antarctic Antarctica King George Island |
genre_facet |
Antarc* Antarctic Antarctica King George Island |
op_relation |
https://hdl.handle.net/20.500.13053/8612 https://doi.org/10.1016/j.procbio.2023.03.003 |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/20.500.13053/861210.1016/j.procbio.2023.03.003 |
container_title |
Process Biochemistry |
container_volume |
129 |
container_start_page |
121 |
op_container_end_page |
132 |
_version_ |
1779310066628820992 |