"Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor "
"Microbial proteases, especially aspartic proteases, are an essential group of enzymes produced from different microorganisms. Microbial proteases have several applications, mainly in the food, beverage, cosmetic, and pharmaceutical industries, due to their efficiency in the processing and in t...
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Language: | English |
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Online Access: | https://hdl.handle.net/20.500.13053/7826 https://doi.org/10.3390/bioengineering9110694 |
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ftunivwiener:oai:https://repositorio.uwiener.edu.pe:20.500.13053/7826 2023-05-15T13:34:24+02:00 "Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor " Machado, Suellen Feitosa, Valker Pillaca-Pullo, Omar Lario, Luciana Sette, Lara Pessoa, Jr., Adalberto Alves, Harley 2022-11-15 application/pdf https://hdl.handle.net/20.500.13053/7826 https://doi.org/10.3390/bioengineering9110694 eng eng MDPI DE https://hdl.handle.net/20.500.13053/7826 https://doi.org/10.3390/bioengineering9110694 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ CC-BY extremophilic psychrophilic proteinase proteolytic bioreactor oxygen transference http://purl.org/pe-repo/ocde/ford#3.03.00 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2022 ftunivwiener https://doi.org/20.500.13053/7826 https://doi.org/10.3390/bioengineering9110694 2023-02-23T01:46:56Z "Microbial proteases, especially aspartic proteases, are an essential group of enzymes produced from different microorganisms. Microbial proteases have several applications, mainly in the food, beverage, cosmetic, and pharmaceutical industries, due to their efficiency in the processing and in the manufacturing stages. The yeast Rhodotorula mucilaginosa CBMAI 1528 was isolated from the Antarctic environment and was previously reported to have higher extracellular aspartic protease production. In addition, advances in the operational conditions of bioreactors for enzyme production are important to reduce the gap associated with scaling−up processes. This is the first study that evaluates the influence of oxygen transference (kLa) on the protease production of R. mucilaginosa yeast. To that end, batch cultures were created in a stirred tank bioreactor using Sabouraud dextrose broth at 25 ◦C for 72 h under kLa values from 18 to 135 h−1 . The results show that kLa (121 h−1 ) obtained at 500 rpm and 1.5 vvm plays an important role in protease production (124.9 U/mL) and productivity (6.784 U/L.h) as well as biomass (10.4 g/L), µmax (0.14 h−1 ) and Yx/s (0.484 g/g). In conclusion, R. mucilaginosa showed high yield production in aerobic culture with the efficiency of protease expression and secretion influenced by kLa. In this sense, our results could be used for further industrial investment." Article in Journal/Newspaper Antarc* Antarctic Repositorial Digital Universidad Wiener - DSpace Principal Uwiener Antarctic The Antarctic Bioengineering 9 11 694 |
institution |
Open Polar |
collection |
Repositorial Digital Universidad Wiener - DSpace Principal Uwiener |
op_collection_id |
ftunivwiener |
language |
English |
topic |
extremophilic psychrophilic proteinase proteolytic bioreactor oxygen transference http://purl.org/pe-repo/ocde/ford#3.03.00 |
spellingShingle |
extremophilic psychrophilic proteinase proteolytic bioreactor oxygen transference http://purl.org/pe-repo/ocde/ford#3.03.00 Machado, Suellen Feitosa, Valker Pillaca-Pullo, Omar Lario, Luciana Sette, Lara Pessoa, Jr., Adalberto Alves, Harley "Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor " |
topic_facet |
extremophilic psychrophilic proteinase proteolytic bioreactor oxygen transference http://purl.org/pe-repo/ocde/ford#3.03.00 |
description |
"Microbial proteases, especially aspartic proteases, are an essential group of enzymes produced from different microorganisms. Microbial proteases have several applications, mainly in the food, beverage, cosmetic, and pharmaceutical industries, due to their efficiency in the processing and in the manufacturing stages. The yeast Rhodotorula mucilaginosa CBMAI 1528 was isolated from the Antarctic environment and was previously reported to have higher extracellular aspartic protease production. In addition, advances in the operational conditions of bioreactors for enzyme production are important to reduce the gap associated with scaling−up processes. This is the first study that evaluates the influence of oxygen transference (kLa) on the protease production of R. mucilaginosa yeast. To that end, batch cultures were created in a stirred tank bioreactor using Sabouraud dextrose broth at 25 ◦C for 72 h under kLa values from 18 to 135 h−1 . The results show that kLa (121 h−1 ) obtained at 500 rpm and 1.5 vvm plays an important role in protease production (124.9 U/mL) and productivity (6.784 U/L.h) as well as biomass (10.4 g/L), µmax (0.14 h−1 ) and Yx/s (0.484 g/g). In conclusion, R. mucilaginosa showed high yield production in aerobic culture with the efficiency of protease expression and secretion influenced by kLa. In this sense, our results could be used for further industrial investment." |
format |
Article in Journal/Newspaper |
author |
Machado, Suellen Feitosa, Valker Pillaca-Pullo, Omar Lario, Luciana Sette, Lara Pessoa, Jr., Adalberto Alves, Harley |
author_facet |
Machado, Suellen Feitosa, Valker Pillaca-Pullo, Omar Lario, Luciana Sette, Lara Pessoa, Jr., Adalberto Alves, Harley |
author_sort |
Machado, Suellen |
title |
"Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor " |
title_short |
"Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor " |
title_full |
"Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor " |
title_fullStr |
"Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor " |
title_full_unstemmed |
"Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor " |
title_sort |
"effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor " |
publisher |
MDPI |
publishDate |
2022 |
url |
https://hdl.handle.net/20.500.13053/7826 https://doi.org/10.3390/bioengineering9110694 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
https://hdl.handle.net/20.500.13053/7826 https://doi.org/10.3390/bioengineering9110694 |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/20.500.13053/7826 https://doi.org/10.3390/bioengineering9110694 |
container_title |
Bioengineering |
container_volume |
9 |
container_issue |
11 |
container_start_page |
694 |
_version_ |
1766052366264565760 |