Changes in total proteins related to chilling injury of nectarine
Low-temperature disorders in nectarines (Prunus persica) can be delayed by storage in atmospheres containing elevated CO₂; however, this benefit is limited to a few cultivars. The storage of produce at low temperatures itself leads to changes in protein synthesis; therefore, this study examined the...
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Stuttgart, Eugen Ulmer GmbH & Co.
2005
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ftunivwestsyd:oai:researchdirect.westernsydney.edu.au:uws_1729 2023-05-15T14:56:36+02:00 Changes in total proteins related to chilling injury of nectarine Uthairatanakij, Apiradee Holford, Paul McGlasson, Barry University of Western Sydney (Host institution) College of Science, Technology and Environment (Host institution) Centre for Horticulture and Plant Sciences (Host institution) 2005 print 7 http://handle.uws.edu.au:8081/1959.7/10539 eng eng Stuttgart, Eugen Ulmer GmbH & Co. European journal of horticultural science--16114426 Vol. 70, no. 6 (2005), pp. 271-277 Prunus persica postharvest storage atmosphere chilling injury 070605 - Post Harvest Horticultural Technologies (incl. Transportation and Storage) journal article 2005 ftunivwestsyd 2020-12-05T17:38:56Z Low-temperature disorders in nectarines (Prunus persica) can be delayed by storage in atmospheres containing elevated CO₂; however, this benefit is limited to a few cultivars. The storage of produce at low temperatures itself leads to changes in protein synthesis; therefore, this study examined the applicability of 2-D electrophoresis to assess differences in protein production in cultivars of nectarine that either do ('Arctic Snow') or do not ('Red Gold') respond to elevated CO₂ levels during storage. In addition, the effect of harvest maturity and their response to added ethylene was assessed. Fruit of the two cultivars were either ripened immediately after harvest at 20°C before protein extraction or stored in air, air + CO₂, air + ethylene or air + CO₂ + ethylene then ripened at 20 °C before protein extraction. 2-D PAGE showed that the expression of approximately 30 proteins varied among cultivars and treatments. Two proteins showed clear differences in expression in both cultivars between fruit ripened immediately after harvest and those that had been stored. A second set of differences in protein expression was found in fruit of 'Red Gold' only and involved 12 proteins whose expression was either reduced or increased by storage: these proteins did not vary in expression in 'Arctic Snow'. A third set of differences in protein expression was observed in 'Arctic Snow' only in atmospheres containing ethylene. Several proteins were down-regulated by ethylene and this down-regulation was negated by the addition of CO₂ to the storage atmosphere. Thus, differences in protein expression can be found between cultivars of nectarines that do or do not respond to the addition of CO₂ to the storage atmosphere and in the cultivar that is known to respond to CO₂, this gas was able to antagonise protein changes mediated by ethylene. Article in Journal/Newspaper Arctic University of Western Sydney (UWS): Research Direct Arctic |
institution |
Open Polar |
collection |
University of Western Sydney (UWS): Research Direct |
op_collection_id |
ftunivwestsyd |
language |
English |
topic |
Prunus persica postharvest storage atmosphere chilling injury 070605 - Post Harvest Horticultural Technologies (incl. Transportation and Storage) |
spellingShingle |
Prunus persica postharvest storage atmosphere chilling injury 070605 - Post Harvest Horticultural Technologies (incl. Transportation and Storage) Uthairatanakij, Apiradee Holford, Paul McGlasson, Barry Changes in total proteins related to chilling injury of nectarine |
topic_facet |
Prunus persica postharvest storage atmosphere chilling injury 070605 - Post Harvest Horticultural Technologies (incl. Transportation and Storage) |
description |
Low-temperature disorders in nectarines (Prunus persica) can be delayed by storage in atmospheres containing elevated CO₂; however, this benefit is limited to a few cultivars. The storage of produce at low temperatures itself leads to changes in protein synthesis; therefore, this study examined the applicability of 2-D electrophoresis to assess differences in protein production in cultivars of nectarine that either do ('Arctic Snow') or do not ('Red Gold') respond to elevated CO₂ levels during storage. In addition, the effect of harvest maturity and their response to added ethylene was assessed. Fruit of the two cultivars were either ripened immediately after harvest at 20°C before protein extraction or stored in air, air + CO₂, air + ethylene or air + CO₂ + ethylene then ripened at 20 °C before protein extraction. 2-D PAGE showed that the expression of approximately 30 proteins varied among cultivars and treatments. Two proteins showed clear differences in expression in both cultivars between fruit ripened immediately after harvest and those that had been stored. A second set of differences in protein expression was found in fruit of 'Red Gold' only and involved 12 proteins whose expression was either reduced or increased by storage: these proteins did not vary in expression in 'Arctic Snow'. A third set of differences in protein expression was observed in 'Arctic Snow' only in atmospheres containing ethylene. Several proteins were down-regulated by ethylene and this down-regulation was negated by the addition of CO₂ to the storage atmosphere. Thus, differences in protein expression can be found between cultivars of nectarines that do or do not respond to the addition of CO₂ to the storage atmosphere and in the cultivar that is known to respond to CO₂, this gas was able to antagonise protein changes mediated by ethylene. |
author2 |
University of Western Sydney (Host institution) College of Science, Technology and Environment (Host institution) Centre for Horticulture and Plant Sciences (Host institution) |
format |
Article in Journal/Newspaper |
author |
Uthairatanakij, Apiradee Holford, Paul McGlasson, Barry |
author_facet |
Uthairatanakij, Apiradee Holford, Paul McGlasson, Barry |
author_sort |
Uthairatanakij, Apiradee |
title |
Changes in total proteins related to chilling injury of nectarine |
title_short |
Changes in total proteins related to chilling injury of nectarine |
title_full |
Changes in total proteins related to chilling injury of nectarine |
title_fullStr |
Changes in total proteins related to chilling injury of nectarine |
title_full_unstemmed |
Changes in total proteins related to chilling injury of nectarine |
title_sort |
changes in total proteins related to chilling injury of nectarine |
publisher |
Stuttgart, Eugen Ulmer GmbH & Co. |
publishDate |
2005 |
url |
http://handle.uws.edu.au:8081/1959.7/10539 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_relation |
European journal of horticultural science--16114426 Vol. 70, no. 6 (2005), pp. 271-277 |
_version_ |
1766328690324537344 |