Adaptation of a mouse Doppler echocardiograph system for assessing cardiac function and thermal performance in a juvenile salmonid
Measures of cardiac performance are pertinent to the study of thermal physiology and exercise in teleosts, particularly as they pertain to migration success. Increased heart rate, stroke volume and cardiac output have previously been linked to improved swimming performance and increased upper therma...
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2021
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ftunivwestonta:oai:ir.lib.uwo.ca:paedpub-2056 2023-10-01T03:54:46+02:00 Adaptation of a mouse Doppler echocardiograph system for assessing cardiac function and thermal performance in a juvenile salmonid Muir, Carlie A. Neff, Bryan D. Damjanovski, Sashko 2021-01-01T08:00:00Z application/pdf https://ir.lib.uwo.ca/paedpub/1048 https://doi.org/10.1093/conphys/coab070 https://ir.lib.uwo.ca/context/paedpub/article/2056/viewcontent/coab070.pdf unknown Scholarship@Western https://ir.lib.uwo.ca/paedpub/1048 doi:10.1093/conphys/coab070 https://ir.lib.uwo.ca/context/paedpub/article/2056/viewcontent/coab070.pdf Paediatrics Publications Atlantic salmon Cardiac function Cardiorespiratory performance Doppler echocardiography Thermal performance article 2021 ftunivwestonta https://doi.org/10.1093/conphys/coab070 2023-09-03T07:41:59Z Measures of cardiac performance are pertinent to the study of thermal physiology and exercise in teleosts, particularly as they pertain to migration success. Increased heart rate, stroke volume and cardiac output have previously been linked to improved swimming performance and increased upper thermal tolerance in anadromous salmonids. To assess thermal performance in fishes, it has become commonplace to measure the response of maximum heart rate to warming using electrocardiograms. However, electrocardiograms do not provide insight into the hemodynamic characteristics of heart function that can impact whole-animal performance. Doppler echocardiography is a popular tool used to examine live animal processes, including real-time cardiac function. This method allows for nonsurgical measurements of blood flow velocity through the heart and has been used to detect abnormalities in cardiovascular function, particularly in mammals. Here, we show how a mouse Doppler echocardiograph system can be adapted for use in a juvenile salmonid over a range of temperatures and timeframes. Using this compact, noninvasive system, we measured maximum heart rate, atrioventricular (AV) blood flow velocity, the early flow-atrial flow ratio and stroke distance in juvenile Atlantic salmon (Salmo salar) during acute warming. Using histologically determined measures of AV valve area, we show how stroke distance measurements obtained with this system can be used to calculate ventricular inflow volume and approximate cardiac output. Further, we show how this Doppler system can be used to determine cardiorespiratory thresholds for thermal performance, which are increasingly being used to predict the consequences that warming water temperatures will have on migratory fishes. Article in Journal/Newspaper Atlantic salmon Salmo salar The University of Western Ontario: Scholarship@Western Conservation Physiology 9 1 |
institution |
Open Polar |
collection |
The University of Western Ontario: Scholarship@Western |
op_collection_id |
ftunivwestonta |
language |
unknown |
topic |
Atlantic salmon Cardiac function Cardiorespiratory performance Doppler echocardiography Thermal performance |
spellingShingle |
Atlantic salmon Cardiac function Cardiorespiratory performance Doppler echocardiography Thermal performance Muir, Carlie A. Neff, Bryan D. Damjanovski, Sashko Adaptation of a mouse Doppler echocardiograph system for assessing cardiac function and thermal performance in a juvenile salmonid |
topic_facet |
Atlantic salmon Cardiac function Cardiorespiratory performance Doppler echocardiography Thermal performance |
description |
Measures of cardiac performance are pertinent to the study of thermal physiology and exercise in teleosts, particularly as they pertain to migration success. Increased heart rate, stroke volume and cardiac output have previously been linked to improved swimming performance and increased upper thermal tolerance in anadromous salmonids. To assess thermal performance in fishes, it has become commonplace to measure the response of maximum heart rate to warming using electrocardiograms. However, electrocardiograms do not provide insight into the hemodynamic characteristics of heart function that can impact whole-animal performance. Doppler echocardiography is a popular tool used to examine live animal processes, including real-time cardiac function. This method allows for nonsurgical measurements of blood flow velocity through the heart and has been used to detect abnormalities in cardiovascular function, particularly in mammals. Here, we show how a mouse Doppler echocardiograph system can be adapted for use in a juvenile salmonid over a range of temperatures and timeframes. Using this compact, noninvasive system, we measured maximum heart rate, atrioventricular (AV) blood flow velocity, the early flow-atrial flow ratio and stroke distance in juvenile Atlantic salmon (Salmo salar) during acute warming. Using histologically determined measures of AV valve area, we show how stroke distance measurements obtained with this system can be used to calculate ventricular inflow volume and approximate cardiac output. Further, we show how this Doppler system can be used to determine cardiorespiratory thresholds for thermal performance, which are increasingly being used to predict the consequences that warming water temperatures will have on migratory fishes. |
format |
Article in Journal/Newspaper |
author |
Muir, Carlie A. Neff, Bryan D. Damjanovski, Sashko |
author_facet |
Muir, Carlie A. Neff, Bryan D. Damjanovski, Sashko |
author_sort |
Muir, Carlie A. |
title |
Adaptation of a mouse Doppler echocardiograph system for assessing cardiac function and thermal performance in a juvenile salmonid |
title_short |
Adaptation of a mouse Doppler echocardiograph system for assessing cardiac function and thermal performance in a juvenile salmonid |
title_full |
Adaptation of a mouse Doppler echocardiograph system for assessing cardiac function and thermal performance in a juvenile salmonid |
title_fullStr |
Adaptation of a mouse Doppler echocardiograph system for assessing cardiac function and thermal performance in a juvenile salmonid |
title_full_unstemmed |
Adaptation of a mouse Doppler echocardiograph system for assessing cardiac function and thermal performance in a juvenile salmonid |
title_sort |
adaptation of a mouse doppler echocardiograph system for assessing cardiac function and thermal performance in a juvenile salmonid |
publisher |
Scholarship@Western |
publishDate |
2021 |
url |
https://ir.lib.uwo.ca/paedpub/1048 https://doi.org/10.1093/conphys/coab070 https://ir.lib.uwo.ca/context/paedpub/article/2056/viewcontent/coab070.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Paediatrics Publications |
op_relation |
https://ir.lib.uwo.ca/paedpub/1048 doi:10.1093/conphys/coab070 https://ir.lib.uwo.ca/context/paedpub/article/2056/viewcontent/coab070.pdf |
op_doi |
https://doi.org/10.1093/conphys/coab070 |
container_title |
Conservation Physiology |
container_volume |
9 |
container_issue |
1 |
_version_ |
1778522674808487936 |