CASA in invertebrates
Sperm movement has been described in several phyla of invertebrates. Yet, sperm motility has only been quantified using computer-aided sperm analysis (CASA-Mot) in externally fertilising species (broadcast spawners) of two phyla, molluscs and echinoderms. In the present study we quantified in detail...
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Online Access: | http://hdl.handle.net/10566/3812 https://doi.org/10.1071/RD17470 |
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ftunivwesterncrr:oai:repository.uwc.ac.za:10566/3812 2023-05-15T15:58:42+02:00 CASA in invertebrates van der Horst, Gerhard Bennett, Monique Bishop, John D. D. 2018 application/pdf http://hdl.handle.net/10566/3812 https://doi.org/10.1071/RD17470 en eng CSIRO Publishing van der Horst, G. et al. (2018). CASA in invertebrates. Reproduction, Fertility and Development, 30: 907–918 1031-3613 http://dx.doi.org/10.1071/RD17470 http://hdl.handle.net/10566/3812 This is the author-version of the article published online at: http://dx.doi.org/10.1071/RD17470 Hyperactivation Motility Spermatozoa Article 2018 ftunivwesterncrr https://doi.org/10.1071/RD17470 2022-04-26T18:56:52Z Sperm movement has been described in several phyla of invertebrates. Yet, sperm motility has only been quantified using computer-aided sperm analysis (CASA-Mot) in externally fertilising species (broadcast spawners) of two phyla, molluscs and echinoderms. In the present study we quantified in detail the nature of the sperm tracks, percentage motility groupings and detailed kinematics of rapid-, medium- and slow-swimming spermatozoa in the oyster Crassostrea gigas and four species never previously studied by CASA-Mot, namely the molluscs Choromytilus meridionalis, Donax serra and Haliotis midae and the echinoderm Parechinus angulosus. A feature common to all these species are the helical tracks, the diameter of which seems to be species specific. Using CASA-Mot, the behaviour of spermatozoa was also studied over time and in the presence of egg water and Ca2+ modulators such as caffeine and procaine hydrochloride. For the first time, we show that hyperactivation can be induced in all species in the presence of egg water (sea water that was mixed with mature eggs and then centrifuged) and/or caffeine, and these hyperactivated sperm tracks were characterised using CASA-Mot. We relate the different patterns of sperm motility and behaviour to reproductive strategies such as broadcast spawning and spermcasting, and briefly review studies using CASA-Mot on other invertebrates. Article in Journal/Newspaper Crassostrea gigas University of the Western Cap: UWC Research Repository Reproduction, Fertility and Development 30 6 907 |
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Open Polar |
collection |
University of the Western Cap: UWC Research Repository |
op_collection_id |
ftunivwesterncrr |
language |
English |
topic |
Hyperactivation Motility Spermatozoa |
spellingShingle |
Hyperactivation Motility Spermatozoa van der Horst, Gerhard Bennett, Monique Bishop, John D. D. CASA in invertebrates |
topic_facet |
Hyperactivation Motility Spermatozoa |
description |
Sperm movement has been described in several phyla of invertebrates. Yet, sperm motility has only been quantified using computer-aided sperm analysis (CASA-Mot) in externally fertilising species (broadcast spawners) of two phyla, molluscs and echinoderms. In the present study we quantified in detail the nature of the sperm tracks, percentage motility groupings and detailed kinematics of rapid-, medium- and slow-swimming spermatozoa in the oyster Crassostrea gigas and four species never previously studied by CASA-Mot, namely the molluscs Choromytilus meridionalis, Donax serra and Haliotis midae and the echinoderm Parechinus angulosus. A feature common to all these species are the helical tracks, the diameter of which seems to be species specific. Using CASA-Mot, the behaviour of spermatozoa was also studied over time and in the presence of egg water and Ca2+ modulators such as caffeine and procaine hydrochloride. For the first time, we show that hyperactivation can be induced in all species in the presence of egg water (sea water that was mixed with mature eggs and then centrifuged) and/or caffeine, and these hyperactivated sperm tracks were characterised using CASA-Mot. We relate the different patterns of sperm motility and behaviour to reproductive strategies such as broadcast spawning and spermcasting, and briefly review studies using CASA-Mot on other invertebrates. |
format |
Article in Journal/Newspaper |
author |
van der Horst, Gerhard Bennett, Monique Bishop, John D. D. |
author_facet |
van der Horst, Gerhard Bennett, Monique Bishop, John D. D. |
author_sort |
van der Horst, Gerhard |
title |
CASA in invertebrates |
title_short |
CASA in invertebrates |
title_full |
CASA in invertebrates |
title_fullStr |
CASA in invertebrates |
title_full_unstemmed |
CASA in invertebrates |
title_sort |
casa in invertebrates |
publisher |
CSIRO Publishing |
publishDate |
2018 |
url |
http://hdl.handle.net/10566/3812 https://doi.org/10.1071/RD17470 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
van der Horst, G. et al. (2018). CASA in invertebrates. Reproduction, Fertility and Development, 30: 907–918 1031-3613 http://dx.doi.org/10.1071/RD17470 http://hdl.handle.net/10566/3812 |
op_rights |
This is the author-version of the article published online at: http://dx.doi.org/10.1071/RD17470 |
op_doi |
https://doi.org/10.1071/RD17470 |
container_title |
Reproduction, Fertility and Development |
container_volume |
30 |
container_issue |
6 |
container_start_page |
907 |
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1766394471198490624 |