High 16S rDNA bacterial diversity in glacial meltwater lake sediment, Bratina Island, Antarctica
The microbial diversity in maritime meltwater pond sediments from Bratina Island, Ross Sea, Antarctica was investigated by 16S rDNA-dependent molecular phylogeny. Investigations of the vertical distribution, phylogenetic composition, and spatial variability of Bacteria and Archaea in the sediment we...
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ftunivwesterncrr:oai:repository.uwc.ac.za:10566/148 2023-05-15T13:36:52+02:00 High 16S rDNA bacterial diversity in glacial meltwater lake sediment, Bratina Island, Antarctica Sjoling, Sara Cowan, Donald A. 2003 application/pdf http://hdl.handle.net/10566/148 en eng Springer Verlag Sjoling, S. & Cowan, D.A. (2003). High 16S rDNA bacterial diversity in glacial meltwater lake sediment, Bratina Island, Antarctica. Extremophiles, 7:275-282. http://hdl.handle.net/10566/148 This is the author post-print version of an article published by Springer http://dx.doi.org/10.1007/s00792-003-0321-z Sediment Antarctica Archaea Microbial diversity Environmental bacteria Article 2003 ftunivwesterncrr https://doi.org/10.1007/s00792-003-0321-z 2022-04-26T18:55:44Z The microbial diversity in maritime meltwater pond sediments from Bratina Island, Ross Sea, Antarctica was investigated by 16S rDNA-dependent molecular phylogeny. Investigations of the vertical distribution, phylogenetic composition, and spatial variability of Bacteria and Archaea in the sediment were carried out. Results revealed the presence of a highly diverse bacterial population and a significantly depthrelated composition. Assessment of 173 partial 16S rDNA clones analyzed by amplified rDNA restriction analysis (ARDRA) using tetrameric restriction enzymes (HinP1I 5'GVCGC3'and Msp I. 5'CVGG3', BioLabs) revealed 153 different bacterial OTUs (operational taxonomic units). However, only seven archaeal OTUs were detected, indicating low archaeal diversity. Based on ARDRA results, 30 bacterial clones were selected for sequencing and the sequenced clones fell into seven major lineages of the domain Bacteria; the a, c, and d subdivisions of Proteobacteria, the Cytophaga–Flavobacterium– Bacteroides, the Spirochaetaceae, and the Actinobacteria. All of the archaeal clones sequenced belonged to the group Crenarchaeota and phylogenetic analysis revealed close relationships with members of the deep-branching Group 1 Marine Archaea. Article in Journal/Newspaper Antarc* Antarctica Bratina Island Ross Sea University of the Western Cap: UWC Research Repository Bratina Island ENVELOPE(165.533,165.533,-78.017,-78.017) Ross Sea Extremophiles 7 4 275 282 |
institution |
Open Polar |
collection |
University of the Western Cap: UWC Research Repository |
op_collection_id |
ftunivwesterncrr |
language |
English |
topic |
Sediment Antarctica Archaea Microbial diversity Environmental bacteria |
spellingShingle |
Sediment Antarctica Archaea Microbial diversity Environmental bacteria Sjoling, Sara Cowan, Donald A. High 16S rDNA bacterial diversity in glacial meltwater lake sediment, Bratina Island, Antarctica |
topic_facet |
Sediment Antarctica Archaea Microbial diversity Environmental bacteria |
description |
The microbial diversity in maritime meltwater pond sediments from Bratina Island, Ross Sea, Antarctica was investigated by 16S rDNA-dependent molecular phylogeny. Investigations of the vertical distribution, phylogenetic composition, and spatial variability of Bacteria and Archaea in the sediment were carried out. Results revealed the presence of a highly diverse bacterial population and a significantly depthrelated composition. Assessment of 173 partial 16S rDNA clones analyzed by amplified rDNA restriction analysis (ARDRA) using tetrameric restriction enzymes (HinP1I 5'GVCGC3'and Msp I. 5'CVGG3', BioLabs) revealed 153 different bacterial OTUs (operational taxonomic units). However, only seven archaeal OTUs were detected, indicating low archaeal diversity. Based on ARDRA results, 30 bacterial clones were selected for sequencing and the sequenced clones fell into seven major lineages of the domain Bacteria; the a, c, and d subdivisions of Proteobacteria, the Cytophaga–Flavobacterium– Bacteroides, the Spirochaetaceae, and the Actinobacteria. All of the archaeal clones sequenced belonged to the group Crenarchaeota and phylogenetic analysis revealed close relationships with members of the deep-branching Group 1 Marine Archaea. |
format |
Article in Journal/Newspaper |
author |
Sjoling, Sara Cowan, Donald A. |
author_facet |
Sjoling, Sara Cowan, Donald A. |
author_sort |
Sjoling, Sara |
title |
High 16S rDNA bacterial diversity in glacial meltwater lake sediment, Bratina Island, Antarctica |
title_short |
High 16S rDNA bacterial diversity in glacial meltwater lake sediment, Bratina Island, Antarctica |
title_full |
High 16S rDNA bacterial diversity in glacial meltwater lake sediment, Bratina Island, Antarctica |
title_fullStr |
High 16S rDNA bacterial diversity in glacial meltwater lake sediment, Bratina Island, Antarctica |
title_full_unstemmed |
High 16S rDNA bacterial diversity in glacial meltwater lake sediment, Bratina Island, Antarctica |
title_sort |
high 16s rdna bacterial diversity in glacial meltwater lake sediment, bratina island, antarctica |
publisher |
Springer Verlag |
publishDate |
2003 |
url |
http://hdl.handle.net/10566/148 |
long_lat |
ENVELOPE(165.533,165.533,-78.017,-78.017) |
geographic |
Bratina Island Ross Sea |
geographic_facet |
Bratina Island Ross Sea |
genre |
Antarc* Antarctica Bratina Island Ross Sea |
genre_facet |
Antarc* Antarctica Bratina Island Ross Sea |
op_source |
http://dx.doi.org/10.1007/s00792-003-0321-z |
op_relation |
Sjoling, S. & Cowan, D.A. (2003). High 16S rDNA bacterial diversity in glacial meltwater lake sediment, Bratina Island, Antarctica. Extremophiles, 7:275-282. http://hdl.handle.net/10566/148 |
op_rights |
This is the author post-print version of an article published by Springer |
op_doi |
https://doi.org/10.1007/s00792-003-0321-z |
container_title |
Extremophiles |
container_volume |
7 |
container_issue |
4 |
container_start_page |
275 |
op_container_end_page |
282 |
_version_ |
1766085207388061696 |