Advanced Genomic Engineering Strategy based on Recombineering Protocols to “Tailor” Escherichia coli Strains
A systematic approach based on bacteriophage Lambda (Lambda Red) and flippase-flippase recognition targets (FLP-FRT) recombinations was proposed for genomic engineering of Escherichia coli. For demonstration purposes, DNA operons containing heterologous genes (i.e. pac encoding E. coli penicillin ac...
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Format: | Master Thesis |
Language: | English |
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University of Waterloo
2011
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Online Access: | http://hdl.handle.net/10012/5982 |