Advanced Genomic Engineering Strategy based on Recombineering Protocols to “Tailor” Escherichia coli Strains

• Main Author: Sukhija, Karan
• Format: Master Thesis
• Language: English
• Published: University of Waterloo 2011
• Subjects: metabolic engineering; e. coli; escherichia coli; recombineering; Chemical Engineering; Lambda; Antarc*; Antarctica
• Online Access: http://hdl.handle.net/10012/5982

A systematic approach based on bacteriophage Lambda (Lambda Red) and flippase-flippase recognition targets (FLP-FRT) recombinations was proposed for genomic engineering of Escherichia coli. For demonstration purposes, DNA operons containing heterologous genes (i.e. pac encoding E. coli penicillin ac...