The Use of Environmental DNA for Understanding Changing Ecosystems: Monitoring Harmful Algal Blooms
Thesis (Master's)--University of Washington, 2019 Environmental DNA is a rapidly developing tool that offers a unique opportunity to sample the communities of an ecosystem in greater depth than other sampling methods. When analyzing environmental samples using general primers and a metabarcodin...
Main Author: | |
---|---|
Other Authors: | |
Format: | Thesis |
Language: | English |
Published: |
2019
|
Subjects: | |
Online Access: | http://hdl.handle.net/1773/44352 |
id |
ftunivwashington:oai:digital.lib.washington.edu:1773/44352 |
---|---|
record_format |
openpolar |
spelling |
ftunivwashington:oai:digital.lib.washington.edu:1773/44352 2023-05-15T17:51:38+02:00 The Use of Environmental DNA for Understanding Changing Ecosystems: Monitoring Harmful Algal Blooms Cribari, Kelly Jean Kelly, Ryan P 2019 application/pdf http://hdl.handle.net/1773/44352 en_US eng Cribari_washington_0250O_20115.pdf http://hdl.handle.net/1773/44352 none Environmental DNA Harmful Algal Bloom Kareniaceae Metabarcoding Polymerase Chain Reaction Molecular biology Marine affairs Thesis 2019 ftunivwashington 2023-03-12T18:59:31Z Thesis (Master's)--University of Washington, 2019 Environmental DNA is a rapidly developing tool that offers a unique opportunity to sample the communities of an ecosystem in greater depth than other sampling methods. When analyzing environmental samples using general primers and a metabarcoding method, researchers are able to detect a multitude of taxa, such as plankton, invertebrates, fish, and mammals, even at low concentrations. Given the changing environment in Puget Sound, biological responses such as the increasing frequency of harmful algal blooms, are a concern. Environmental DNA can help detect and track harmful algal species, as it has been used for other harmful or invasive species. In this study, I use environmental DNA to detect a potentially toxic dinoflagellate in the family Kareniaceae, which is not well reported in Puget Sound, and assess the environmental conditions with which it is associated. In the present dataset, the species occurs only at depth, and in particular, in low-pH, low-dissolved-oxygen, and high-salinity conditions. I then use logistic regression to predict the presence of the dinoflagellate given the data in hand; the resulting model suggests that a change in water pH from 7.8 to 7.4 increases the probability of Kareniaceae sp. presence by from 29% to 95%. With decreasing pH levels within Puget Sound, the continuing progression of ocean acidification could lead to bloom conditions for the taxon. This method could become a first-step monitoring tool for species such as Kareniaceae sp. and to focus investigative efforts to prevent HABs. Thesis Ocean acidification University of Washington, Seattle: ResearchWorks |
institution |
Open Polar |
collection |
University of Washington, Seattle: ResearchWorks |
op_collection_id |
ftunivwashington |
language |
English |
topic |
Environmental DNA Harmful Algal Bloom Kareniaceae Metabarcoding Polymerase Chain Reaction Molecular biology Marine affairs |
spellingShingle |
Environmental DNA Harmful Algal Bloom Kareniaceae Metabarcoding Polymerase Chain Reaction Molecular biology Marine affairs Cribari, Kelly Jean The Use of Environmental DNA for Understanding Changing Ecosystems: Monitoring Harmful Algal Blooms |
topic_facet |
Environmental DNA Harmful Algal Bloom Kareniaceae Metabarcoding Polymerase Chain Reaction Molecular biology Marine affairs |
description |
Thesis (Master's)--University of Washington, 2019 Environmental DNA is a rapidly developing tool that offers a unique opportunity to sample the communities of an ecosystem in greater depth than other sampling methods. When analyzing environmental samples using general primers and a metabarcoding method, researchers are able to detect a multitude of taxa, such as plankton, invertebrates, fish, and mammals, even at low concentrations. Given the changing environment in Puget Sound, biological responses such as the increasing frequency of harmful algal blooms, are a concern. Environmental DNA can help detect and track harmful algal species, as it has been used for other harmful or invasive species. In this study, I use environmental DNA to detect a potentially toxic dinoflagellate in the family Kareniaceae, which is not well reported in Puget Sound, and assess the environmental conditions with which it is associated. In the present dataset, the species occurs only at depth, and in particular, in low-pH, low-dissolved-oxygen, and high-salinity conditions. I then use logistic regression to predict the presence of the dinoflagellate given the data in hand; the resulting model suggests that a change in water pH from 7.8 to 7.4 increases the probability of Kareniaceae sp. presence by from 29% to 95%. With decreasing pH levels within Puget Sound, the continuing progression of ocean acidification could lead to bloom conditions for the taxon. This method could become a first-step monitoring tool for species such as Kareniaceae sp. and to focus investigative efforts to prevent HABs. |
author2 |
Kelly, Ryan P |
format |
Thesis |
author |
Cribari, Kelly Jean |
author_facet |
Cribari, Kelly Jean |
author_sort |
Cribari, Kelly Jean |
title |
The Use of Environmental DNA for Understanding Changing Ecosystems: Monitoring Harmful Algal Blooms |
title_short |
The Use of Environmental DNA for Understanding Changing Ecosystems: Monitoring Harmful Algal Blooms |
title_full |
The Use of Environmental DNA for Understanding Changing Ecosystems: Monitoring Harmful Algal Blooms |
title_fullStr |
The Use of Environmental DNA for Understanding Changing Ecosystems: Monitoring Harmful Algal Blooms |
title_full_unstemmed |
The Use of Environmental DNA for Understanding Changing Ecosystems: Monitoring Harmful Algal Blooms |
title_sort |
use of environmental dna for understanding changing ecosystems: monitoring harmful algal blooms |
publishDate |
2019 |
url |
http://hdl.handle.net/1773/44352 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
Cribari_washington_0250O_20115.pdf http://hdl.handle.net/1773/44352 |
op_rights |
none |
_version_ |
1766158849512833024 |