DNA methylation variation in gametes and larvae of the Pacific oyster, Crassostrea gigas
Thesis (Master's)--University of Washington, 2014 Epigenetics describes DNA modifications that change gene expression without altering the underlying nucleotide sequence. Epigenetic mechanisms such as DNA methylation can change genome function under external influences. The focus of this projec...
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ftunivwashington:oai:digital.lib.washington.edu:1773/27478 2023-05-15T15:58:46+02:00 DNA methylation variation in gametes and larvae of the Pacific oyster, Crassostrea gigas Olson, Claire Ellis Roberts, Steven B 2014 application/pdf http://hdl.handle.net/1773/27478 en_US eng Olson_washington_0250O_13948.pdf http://hdl.handle.net/1773/27478 Copyright is held by the individual authors. bivalves DNA methylation epigenetics oyster Fisheries and aquatic sciences Genetics fisheries Thesis 2014 ftunivwashington 2023-03-12T18:52:08Z Thesis (Master's)--University of Washington, 2014 Epigenetics describes DNA modifications that change gene expression without altering the underlying nucleotide sequence. Epigenetic mechanisms such as DNA methylation can change genome function under external influences. The focus of this project is examining one epigenetic modification, DNA methylation, in oysters. DNA methylation has been well studied in vertebrates, but remains understudied in invertebrates. Furthermore, the amounts and functions of DNA methylation in organisms are extremely diverse and variable across taxa. This thesis determines patterns of DNA methylation in C. gigas to elucidate the functional role of DNA methylation. The first chapter examines the genome-wide DNA methylation profile in C. gigas male gamete cells using whole-genome bisulfite sequencing. RNA-Seq analysis was also performed on the same tissue to provide insight into the mechanisms by which DNA methylation impacts transcriptional processes. The work presented in Chapter 2 examines methylation patterns of C. gigas during early oyster developmental stages (spermatozoa and larvae). Together these data were used to test the predictions that DNA methylation is involved in gene regulatory activity and is heritable. This work also describes individual variation, parental transmission and developmental patterns of DNA methylation in oysters. Our results indicate a positive relationship between DNA methylation and gene expression, and that DNA methylation patterns are inherited in oysters. Thesis Crassostrea gigas Pacific oyster University of Washington, Seattle: ResearchWorks Pacific |
institution |
Open Polar |
collection |
University of Washington, Seattle: ResearchWorks |
op_collection_id |
ftunivwashington |
language |
English |
topic |
bivalves DNA methylation epigenetics oyster Fisheries and aquatic sciences Genetics fisheries |
spellingShingle |
bivalves DNA methylation epigenetics oyster Fisheries and aquatic sciences Genetics fisheries Olson, Claire Ellis DNA methylation variation in gametes and larvae of the Pacific oyster, Crassostrea gigas |
topic_facet |
bivalves DNA methylation epigenetics oyster Fisheries and aquatic sciences Genetics fisheries |
description |
Thesis (Master's)--University of Washington, 2014 Epigenetics describes DNA modifications that change gene expression without altering the underlying nucleotide sequence. Epigenetic mechanisms such as DNA methylation can change genome function under external influences. The focus of this project is examining one epigenetic modification, DNA methylation, in oysters. DNA methylation has been well studied in vertebrates, but remains understudied in invertebrates. Furthermore, the amounts and functions of DNA methylation in organisms are extremely diverse and variable across taxa. This thesis determines patterns of DNA methylation in C. gigas to elucidate the functional role of DNA methylation. The first chapter examines the genome-wide DNA methylation profile in C. gigas male gamete cells using whole-genome bisulfite sequencing. RNA-Seq analysis was also performed on the same tissue to provide insight into the mechanisms by which DNA methylation impacts transcriptional processes. The work presented in Chapter 2 examines methylation patterns of C. gigas during early oyster developmental stages (spermatozoa and larvae). Together these data were used to test the predictions that DNA methylation is involved in gene regulatory activity and is heritable. This work also describes individual variation, parental transmission and developmental patterns of DNA methylation in oysters. Our results indicate a positive relationship between DNA methylation and gene expression, and that DNA methylation patterns are inherited in oysters. |
author2 |
Roberts, Steven B |
format |
Thesis |
author |
Olson, Claire Ellis |
author_facet |
Olson, Claire Ellis |
author_sort |
Olson, Claire Ellis |
title |
DNA methylation variation in gametes and larvae of the Pacific oyster, Crassostrea gigas |
title_short |
DNA methylation variation in gametes and larvae of the Pacific oyster, Crassostrea gigas |
title_full |
DNA methylation variation in gametes and larvae of the Pacific oyster, Crassostrea gigas |
title_fullStr |
DNA methylation variation in gametes and larvae of the Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
DNA methylation variation in gametes and larvae of the Pacific oyster, Crassostrea gigas |
title_sort |
dna methylation variation in gametes and larvae of the pacific oyster, crassostrea gigas |
publishDate |
2014 |
url |
http://hdl.handle.net/1773/27478 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
Olson_washington_0250O_13948.pdf http://hdl.handle.net/1773/27478 |
op_rights |
Copyright is held by the individual authors. |
_version_ |
1766394536601321472 |