High-level diversity of tailed phages, eukaryote-associated viruses, and virophage-like elements in the metaviromes of Antarctic soils
The metaviromes of two distinct Antarctic hyperarid desert soil communities have been characterized. Hypolithic communities, cyanobacterium-dominated assemblages situated on the ventral surfaces of quartz pebbles embedded in the desert pavement, showed higher virus diversity than surface soils, whic...
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ftunivwaikato:oai:researchcommons.waikato.ac.nz:10289/9592 2024-02-11T09:58:50+01:00 High-level diversity of tailed phages, eukaryote-associated viruses, and virophage-like elements in the metaviromes of Antarctic soils Zablocki, Oliver van Zyl, Lonnie Adriaenssens, Evelien M. Rubagotti, Enrico Tuffin, Marla I. Cary, S. Craig Cowan, Don A. 2014-11-01 application/pdf https://hdl.handle.net/10289/9592 https://doi.org/10.1128/AEM.01525-14 en eng American Society Microbiology http://aem.asm.org/content/80/22/6888 Applied and Environmental Microbiology Zablocki, O., van Zyl, L., Adriaenssens, E. M., Rubagotti, E., Tuffin, M. I., Cary, S. C., & Cowan, D. A. (2014). High-level diversity of tailed phages, eukaryote-associated viruses, and virophage-like elements in the metaviromes of Antarctic soils. Applied and Environmental Microbiology, 80(22), 6888–6897. http://doi.org/10.1128/AEM.01525-14 https://hdl.handle.net/10289/9592 doi:10.1128/AEM.01525-14 1098-5336 © 2014 American Society for Microbiology. Used with permission. Journal Article 2014 ftunivwaikato https://doi.org/10.1128/AEM.01525-14 2024-01-16T18:25:20Z The metaviromes of two distinct Antarctic hyperarid desert soil communities have been characterized. Hypolithic communities, cyanobacterium-dominated assemblages situated on the ventral surfaces of quartz pebbles embedded in the desert pavement, showed higher virus diversity than surface soils, which correlated with previous bacterial community studies. Prokaryotic viruses (i.e., phages) represented the largest viral component (particularly Mycobacterium phages) in both habitats, with an identical hierarchical sequence abundance of families of tailed phages (Siphoviridae > Myoviridae > Podoviridae). No archaeal viruses were found. Unexpectedly, cyanophages were poorly represented in both metaviromes and were phylogenetically distant from currently characterized cyanophages. Putative phage genomes were assembled and showed a high level of unaffiliated genes, mostly from hypolithic viruses. Moreover, unusual gene arrangements in which eukaryotic and prokaryotic virus-derived genes were found within identical genome segments were observed. Phycodnaviridae and Mimiviridae viruses were the second-most-abundant taxa and more numerous within open soil. Novel virophage-like sequences (within the Sputnik clade) were identified. These findings highlight high-level virus diversity and novel species discovery potential within Antarctic hyperarid soils and may serve as a starting point for future studies targeting specific viral groups. Article in Journal/Newspaper Antarc* Antarctic The University of Waikato: Research Commons Antarctic Sputnik ENVELOPE(66.167,66.167,-70.833,-70.833) Applied and Environmental Microbiology 80 22 6888 6897 |
institution |
Open Polar |
collection |
The University of Waikato: Research Commons |
op_collection_id |
ftunivwaikato |
language |
English |
description |
The metaviromes of two distinct Antarctic hyperarid desert soil communities have been characterized. Hypolithic communities, cyanobacterium-dominated assemblages situated on the ventral surfaces of quartz pebbles embedded in the desert pavement, showed higher virus diversity than surface soils, which correlated with previous bacterial community studies. Prokaryotic viruses (i.e., phages) represented the largest viral component (particularly Mycobacterium phages) in both habitats, with an identical hierarchical sequence abundance of families of tailed phages (Siphoviridae > Myoviridae > Podoviridae). No archaeal viruses were found. Unexpectedly, cyanophages were poorly represented in both metaviromes and were phylogenetically distant from currently characterized cyanophages. Putative phage genomes were assembled and showed a high level of unaffiliated genes, mostly from hypolithic viruses. Moreover, unusual gene arrangements in which eukaryotic and prokaryotic virus-derived genes were found within identical genome segments were observed. Phycodnaviridae and Mimiviridae viruses were the second-most-abundant taxa and more numerous within open soil. Novel virophage-like sequences (within the Sputnik clade) were identified. These findings highlight high-level virus diversity and novel species discovery potential within Antarctic hyperarid soils and may serve as a starting point for future studies targeting specific viral groups. |
format |
Article in Journal/Newspaper |
author |
Zablocki, Oliver van Zyl, Lonnie Adriaenssens, Evelien M. Rubagotti, Enrico Tuffin, Marla I. Cary, S. Craig Cowan, Don A. |
spellingShingle |
Zablocki, Oliver van Zyl, Lonnie Adriaenssens, Evelien M. Rubagotti, Enrico Tuffin, Marla I. Cary, S. Craig Cowan, Don A. High-level diversity of tailed phages, eukaryote-associated viruses, and virophage-like elements in the metaviromes of Antarctic soils |
author_facet |
Zablocki, Oliver van Zyl, Lonnie Adriaenssens, Evelien M. Rubagotti, Enrico Tuffin, Marla I. Cary, S. Craig Cowan, Don A. |
author_sort |
Zablocki, Oliver |
title |
High-level diversity of tailed phages, eukaryote-associated viruses, and virophage-like elements in the metaviromes of Antarctic soils |
title_short |
High-level diversity of tailed phages, eukaryote-associated viruses, and virophage-like elements in the metaviromes of Antarctic soils |
title_full |
High-level diversity of tailed phages, eukaryote-associated viruses, and virophage-like elements in the metaviromes of Antarctic soils |
title_fullStr |
High-level diversity of tailed phages, eukaryote-associated viruses, and virophage-like elements in the metaviromes of Antarctic soils |
title_full_unstemmed |
High-level diversity of tailed phages, eukaryote-associated viruses, and virophage-like elements in the metaviromes of Antarctic soils |
title_sort |
high-level diversity of tailed phages, eukaryote-associated viruses, and virophage-like elements in the metaviromes of antarctic soils |
publisher |
American Society Microbiology |
publishDate |
2014 |
url |
https://hdl.handle.net/10289/9592 https://doi.org/10.1128/AEM.01525-14 |
long_lat |
ENVELOPE(66.167,66.167,-70.833,-70.833) |
geographic |
Antarctic Sputnik |
geographic_facet |
Antarctic Sputnik |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
http://aem.asm.org/content/80/22/6888 Applied and Environmental Microbiology Zablocki, O., van Zyl, L., Adriaenssens, E. M., Rubagotti, E., Tuffin, M. I., Cary, S. C., & Cowan, D. A. (2014). High-level diversity of tailed phages, eukaryote-associated viruses, and virophage-like elements in the metaviromes of Antarctic soils. Applied and Environmental Microbiology, 80(22), 6888–6897. http://doi.org/10.1128/AEM.01525-14 https://hdl.handle.net/10289/9592 doi:10.1128/AEM.01525-14 1098-5336 |
op_rights |
© 2014 American Society for Microbiology. Used with permission. |
op_doi |
https://doi.org/10.1128/AEM.01525-14 |
container_title |
Applied and Environmental Microbiology |
container_volume |
80 |
container_issue |
22 |
container_start_page |
6888 |
op_container_end_page |
6897 |
_version_ |
1790594615451058176 |