Diurnal changes in the heterotrophic uptake of glycolate and glucose in two lakes
A study was made of diurnal changes in the heterotrophic uptake kinetics of both ¹⁴C-glucose and ¹⁴C- glycolate in two small stratified lakes, Lake Rotomanuka in New Zealand and Lake Fryxell in Antarctica. The purpose of the investigation was to study the effect that diurnal changes in the extracell...
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ftunivwaikato:oai:researchcommons.waikato.ac.nz:10289/8228 2023-05-15T14:00:59+02:00 Diurnal changes in the heterotrophic uptake of glycolate and glucose in two lakes Rawley, Brian Andrew Harfoot, C.G. 2013-11-24T22:38:56Z application/pdf https://hdl.handle.net/10289/8228 en eng University of Waikato https://hdl.handle.net/10289/8228 All items in Research Commons are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated. Antarctica Thesis 2013 ftunivwaikato 2022-03-29T15:13:19Z A study was made of diurnal changes in the heterotrophic uptake kinetics of both ¹⁴C-glucose and ¹⁴C- glycolate in two small stratified lakes, Lake Rotomanuka in New Zealand and Lake Fryxell in Antarctica. The purpose of the investigation was to study the effect that diurnal changes in the extracellular release of certain substrates by algae might have on the uptake of those substrates by aquatic microheterotrophs. The heterotrophic uptake technique of Parsons and Strickland.(196l) was modified to provide a technique which was more convenient for field-work. In an effort to exclude the effects of migration and heterogeneous population distribution on substrate uptake, the microbial population in each lake was observed using a combination of (a) direct counts of bacteria (using acridine orange staining and epi-fluorescent microscopy); (b) measurement of chlorophyll α and bacterio-chlorophyll and (c) measurement of the reduction of INT dye. The natural concentration of glycolate was measured on some occasions using a modification of the technique of Shah and Wright (1974). Thesis Antarc* Antarctica The University of Waikato: Research Commons New Zealand Fryxell ENVELOPE(163.183,163.183,-77.617,-77.617) Lake Fryxell ENVELOPE(163.183,163.183,-77.617,-77.617) |
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The University of Waikato: Research Commons |
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ftunivwaikato |
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English |
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Antarctica |
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Antarctica Rawley, Brian Andrew Diurnal changes in the heterotrophic uptake of glycolate and glucose in two lakes |
topic_facet |
Antarctica |
description |
A study was made of diurnal changes in the heterotrophic uptake kinetics of both ¹⁴C-glucose and ¹⁴C- glycolate in two small stratified lakes, Lake Rotomanuka in New Zealand and Lake Fryxell in Antarctica. The purpose of the investigation was to study the effect that diurnal changes in the extracellular release of certain substrates by algae might have on the uptake of those substrates by aquatic microheterotrophs. The heterotrophic uptake technique of Parsons and Strickland.(196l) was modified to provide a technique which was more convenient for field-work. In an effort to exclude the effects of migration and heterogeneous population distribution on substrate uptake, the microbial population in each lake was observed using a combination of (a) direct counts of bacteria (using acridine orange staining and epi-fluorescent microscopy); (b) measurement of chlorophyll α and bacterio-chlorophyll and (c) measurement of the reduction of INT dye. The natural concentration of glycolate was measured on some occasions using a modification of the technique of Shah and Wright (1974). |
author2 |
Harfoot, C.G. |
format |
Thesis |
author |
Rawley, Brian Andrew |
author_facet |
Rawley, Brian Andrew |
author_sort |
Rawley, Brian Andrew |
title |
Diurnal changes in the heterotrophic uptake of glycolate and glucose in two lakes |
title_short |
Diurnal changes in the heterotrophic uptake of glycolate and glucose in two lakes |
title_full |
Diurnal changes in the heterotrophic uptake of glycolate and glucose in two lakes |
title_fullStr |
Diurnal changes in the heterotrophic uptake of glycolate and glucose in two lakes |
title_full_unstemmed |
Diurnal changes in the heterotrophic uptake of glycolate and glucose in two lakes |
title_sort |
diurnal changes in the heterotrophic uptake of glycolate and glucose in two lakes |
publisher |
University of Waikato |
publishDate |
2013 |
url |
https://hdl.handle.net/10289/8228 |
long_lat |
ENVELOPE(163.183,163.183,-77.617,-77.617) ENVELOPE(163.183,163.183,-77.617,-77.617) |
geographic |
New Zealand Fryxell Lake Fryxell |
geographic_facet |
New Zealand Fryxell Lake Fryxell |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
https://hdl.handle.net/10289/8228 |
op_rights |
All items in Research Commons are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated. |
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1766270393523372032 |