Characterisation of Antarctic cyanobacteria and comparison with New Zealand strains
Cyanobacterial mats are common in Antarctic lakes, ponds and on moist soils. The species comprising these mats have adapted to tolerate extreme conditions (e.g. high salinities and UV radiation, freezing and extended periods of darkness). In this study, cyanobacterial mats were collected from shallo...
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ftunivwaikato:oai:researchcommons.waikato.ac.nz:10289/7364 2023-05-15T14:00:59+02:00 Characterisation of Antarctic cyanobacteria and comparison with New Zealand strains Martineau, Edouard Wood, Susanna A. Miller, Matthew R. Jungblut, Anne D. Hawes, Ian Webster-Brown, Jenny Packer, Michael A. 2013 https://hdl.handle.net/10289/7364 https://doi.org/10.1007/s10750-013-1473-1 en eng Springer Hydrobiologia Martineau, E., Wood, S. A., Miller, M. R., Jungblut, A. D., Hawes, I., Webster-Brown, J., & Packer, M. A. (2013). Characterisation of Antarctic cyanobacteria and comparison with New Zealand strains. Hydrobiologia, 711(1), 139-154. 0018-8158 https://hdl.handle.net/10289/7364 doi:10.1007/s10750-013-1473-1 16S rRNA gene Eicosapentaenoic acid Fatty acid Hexadecatrienoic acid Hydrocarbons Lipid class Journal Article 2013 ftunivwaikato https://doi.org/10.1007/s10750-013-1473-1 2022-03-29T15:12:55Z Cyanobacterial mats are common in Antarctic lakes, ponds and on moist soils. The species comprising these mats have adapted to tolerate extreme conditions (e.g. high salinities and UV radiation, freezing and extended periods of darkness). In this study, cyanobacterial mats were collected from shallow melt-water ponds in Pyramid Trough in Southern Victoria Land, Antarctica. Eight strains were isolated and characterised by morphological and molecular (16S rRNA gene sequences) techniques and their fatty acid methyl ester (FAME) and lipid class profiles determined. These data were compared to parallel information obtained from cyanobacterial cultures isolated from New Zealand. In general, the morphological and molecular characterisation complemented each other, and the Antarctic strains identified belonged to the orders: Oscillatoriales (six), Nostocales (one) and Chroococcales (one). Two of the Antarctic strains (CYN67 and CYN68) showed low similarity (<96% 16S rRNA gene sequence) when compared to other cultured cyanobacteria. The fatty acid (FA) profiles from the Antarctic and New Zealand strains shared many similarities with palmitic (C16:0), stearic (C18:0) and oleic acid (C18:1n-9) most abundant. In contrast, the lipid class analysis differed among geographic locations with Antarctic strains containing higher amounts of hydrocarbons and eicosapentaenoic and hexadecatrienoic acids. Article in Journal/Newspaper Antarc* Antarctic Antarctica Victoria Land The University of Waikato: Research Commons Antarctic The Antarctic Victoria Land New Zealand Pyramid ENVELOPE(157.300,157.300,-81.333,-81.333) Pyramid Trough ENVELOPE(163.450,163.450,-78.300,-78.300) Hydrobiologia 711 1 139 154 |
institution |
Open Polar |
collection |
The University of Waikato: Research Commons |
op_collection_id |
ftunivwaikato |
language |
English |
topic |
16S rRNA gene Eicosapentaenoic acid Fatty acid Hexadecatrienoic acid Hydrocarbons Lipid class |
spellingShingle |
16S rRNA gene Eicosapentaenoic acid Fatty acid Hexadecatrienoic acid Hydrocarbons Lipid class Martineau, Edouard Wood, Susanna A. Miller, Matthew R. Jungblut, Anne D. Hawes, Ian Webster-Brown, Jenny Packer, Michael A. Characterisation of Antarctic cyanobacteria and comparison with New Zealand strains |
topic_facet |
16S rRNA gene Eicosapentaenoic acid Fatty acid Hexadecatrienoic acid Hydrocarbons Lipid class |
description |
Cyanobacterial mats are common in Antarctic lakes, ponds and on moist soils. The species comprising these mats have adapted to tolerate extreme conditions (e.g. high salinities and UV radiation, freezing and extended periods of darkness). In this study, cyanobacterial mats were collected from shallow melt-water ponds in Pyramid Trough in Southern Victoria Land, Antarctica. Eight strains were isolated and characterised by morphological and molecular (16S rRNA gene sequences) techniques and their fatty acid methyl ester (FAME) and lipid class profiles determined. These data were compared to parallel information obtained from cyanobacterial cultures isolated from New Zealand. In general, the morphological and molecular characterisation complemented each other, and the Antarctic strains identified belonged to the orders: Oscillatoriales (six), Nostocales (one) and Chroococcales (one). Two of the Antarctic strains (CYN67 and CYN68) showed low similarity (<96% 16S rRNA gene sequence) when compared to other cultured cyanobacteria. The fatty acid (FA) profiles from the Antarctic and New Zealand strains shared many similarities with palmitic (C16:0), stearic (C18:0) and oleic acid (C18:1n-9) most abundant. In contrast, the lipid class analysis differed among geographic locations with Antarctic strains containing higher amounts of hydrocarbons and eicosapentaenoic and hexadecatrienoic acids. |
format |
Article in Journal/Newspaper |
author |
Martineau, Edouard Wood, Susanna A. Miller, Matthew R. Jungblut, Anne D. Hawes, Ian Webster-Brown, Jenny Packer, Michael A. |
author_facet |
Martineau, Edouard Wood, Susanna A. Miller, Matthew R. Jungblut, Anne D. Hawes, Ian Webster-Brown, Jenny Packer, Michael A. |
author_sort |
Martineau, Edouard |
title |
Characterisation of Antarctic cyanobacteria and comparison with New Zealand strains |
title_short |
Characterisation of Antarctic cyanobacteria and comparison with New Zealand strains |
title_full |
Characterisation of Antarctic cyanobacteria and comparison with New Zealand strains |
title_fullStr |
Characterisation of Antarctic cyanobacteria and comparison with New Zealand strains |
title_full_unstemmed |
Characterisation of Antarctic cyanobacteria and comparison with New Zealand strains |
title_sort |
characterisation of antarctic cyanobacteria and comparison with new zealand strains |
publisher |
Springer |
publishDate |
2013 |
url |
https://hdl.handle.net/10289/7364 https://doi.org/10.1007/s10750-013-1473-1 |
long_lat |
ENVELOPE(157.300,157.300,-81.333,-81.333) ENVELOPE(163.450,163.450,-78.300,-78.300) |
geographic |
Antarctic The Antarctic Victoria Land New Zealand Pyramid Pyramid Trough |
geographic_facet |
Antarctic The Antarctic Victoria Land New Zealand Pyramid Pyramid Trough |
genre |
Antarc* Antarctic Antarctica Victoria Land |
genre_facet |
Antarc* Antarctic Antarctica Victoria Land |
op_relation |
Hydrobiologia Martineau, E., Wood, S. A., Miller, M. R., Jungblut, A. D., Hawes, I., Webster-Brown, J., & Packer, M. A. (2013). Characterisation of Antarctic cyanobacteria and comparison with New Zealand strains. Hydrobiologia, 711(1), 139-154. 0018-8158 https://hdl.handle.net/10289/7364 doi:10.1007/s10750-013-1473-1 |
op_doi |
https://doi.org/10.1007/s10750-013-1473-1 |
container_title |
Hydrobiologia |
container_volume |
711 |
container_issue |
1 |
container_start_page |
139 |
op_container_end_page |
154 |
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1766270391695704064 |