Diverse and highly active diazotrophic assemblages inhabit ephemerally wetted soils of the Antarctic Dry Valleys
Eolian transport of biomass from ephemerally wetted soils, associated with summer glacial meltwater runoffs and lake edges, to low-productivity areas of the Antarctic Dry Valleys (DV) has been postulated to be an important source of organic matter (fixed nitrogen and fixed carbon) to the entire DV e...
Published in: | FEMS Microbiology Ecology |
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2012
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Online Access: | https://hdl.handle.net/10289/6775 https://doi.org/10.1111/j.1574-6941.2012.01390.x |
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ftunivwaikato:oai:researchcommons.waikato.ac.nz:10289/6775 2023-11-12T04:06:16+01:00 Diverse and highly active diazotrophic assemblages inhabit ephemerally wetted soils of the Antarctic Dry Valleys Niederberger, Thomas D. Sohm, Jill A. Tirindelli, Joëlle Gunderson, Troy Capone, Douglas G. Carpenter, Edward J. Cary, S. Craig England 2012 https://hdl.handle.net/10289/6775 https://doi.org/10.1111/j.1574-6941.2012.01390.x en eng Blackwell Publishing Ltd FEMS Microbiology Ecology Niederberger, T. D., Sohm, J. A., Tirindelli, J., Gunderson, T., Capone, D. G., Carpenter, E. J. and Cary, C.S. (2012), Diverse and highly active diazotrophic assemblages inhabit ephemerally wetted soils of the Antarctic Dry Valleys. FEMS Microbiology Ecology, 82(2), 376-390. 1574-6941 https://hdl.handle.net/10289/6775 doi:10.1111/j.1574-6941.2012.01390.x diazotrophs Dry Valley Antarctica activity community Journal Article 2012 ftunivwaikato https://doi.org/10.1111/j.1574-6941.2012.01390.x 2023-10-17T17:24:10Z Eolian transport of biomass from ephemerally wetted soils, associated with summer glacial meltwater runoffs and lake edges, to low-productivity areas of the Antarctic Dry Valleys (DV) has been postulated to be an important source of organic matter (fixed nitrogen and fixed carbon) to the entire DV ecosystem. However, descriptions and identification of the microbial members responsible for N₂ fixation within these wetted sites are limited. In this study, N₂ fixers from wetted soils were identified by direct nifH gene sequencing and their in situ N₂ fixation activities documented via acetylene reduction and RNA-based quantitative PCR assays. Shannon-index nifH diversity levels ranged between 1.8 and 2.6 and included the expected cyanobacterial signatures and a large number of phylotypes related to the gamma-, beta-, alpha-, and delta-proteobacteria. N₂ fixation rates ranged between approximately 0.5 and 6 nmol N cm⁻³ h⁻¹ with measurements indicating that approximately 50% of this activity was linked with sulfate reduction at some sites. Comparisons with proximal dry soils also suggested that these communities are not ubiquitously distributed, and conditions unrelated to moisture content may define the composition, diversity, or habitat suitability of the microbial communities within wetted soils of the DVs. Article in Journal/Newspaper Antarc* Antarctic Antarctica The University of Waikato: Research Commons Antarctic The Antarctic FEMS Microbiology Ecology 82 2 376 390 |
institution |
Open Polar |
collection |
The University of Waikato: Research Commons |
op_collection_id |
ftunivwaikato |
language |
English |
topic |
diazotrophs Dry Valley Antarctica activity community |
spellingShingle |
diazotrophs Dry Valley Antarctica activity community Niederberger, Thomas D. Sohm, Jill A. Tirindelli, Joëlle Gunderson, Troy Capone, Douglas G. Carpenter, Edward J. Cary, S. Craig Diverse and highly active diazotrophic assemblages inhabit ephemerally wetted soils of the Antarctic Dry Valleys |
topic_facet |
diazotrophs Dry Valley Antarctica activity community |
description |
Eolian transport of biomass from ephemerally wetted soils, associated with summer glacial meltwater runoffs and lake edges, to low-productivity areas of the Antarctic Dry Valleys (DV) has been postulated to be an important source of organic matter (fixed nitrogen and fixed carbon) to the entire DV ecosystem. However, descriptions and identification of the microbial members responsible for N₂ fixation within these wetted sites are limited. In this study, N₂ fixers from wetted soils were identified by direct nifH gene sequencing and their in situ N₂ fixation activities documented via acetylene reduction and RNA-based quantitative PCR assays. Shannon-index nifH diversity levels ranged between 1.8 and 2.6 and included the expected cyanobacterial signatures and a large number of phylotypes related to the gamma-, beta-, alpha-, and delta-proteobacteria. N₂ fixation rates ranged between approximately 0.5 and 6 nmol N cm⁻³ h⁻¹ with measurements indicating that approximately 50% of this activity was linked with sulfate reduction at some sites. Comparisons with proximal dry soils also suggested that these communities are not ubiquitously distributed, and conditions unrelated to moisture content may define the composition, diversity, or habitat suitability of the microbial communities within wetted soils of the DVs. |
format |
Article in Journal/Newspaper |
author |
Niederberger, Thomas D. Sohm, Jill A. Tirindelli, Joëlle Gunderson, Troy Capone, Douglas G. Carpenter, Edward J. Cary, S. Craig |
author_facet |
Niederberger, Thomas D. Sohm, Jill A. Tirindelli, Joëlle Gunderson, Troy Capone, Douglas G. Carpenter, Edward J. Cary, S. Craig |
author_sort |
Niederberger, Thomas D. |
title |
Diverse and highly active diazotrophic assemblages inhabit ephemerally wetted soils of the Antarctic Dry Valleys |
title_short |
Diverse and highly active diazotrophic assemblages inhabit ephemerally wetted soils of the Antarctic Dry Valleys |
title_full |
Diverse and highly active diazotrophic assemblages inhabit ephemerally wetted soils of the Antarctic Dry Valleys |
title_fullStr |
Diverse and highly active diazotrophic assemblages inhabit ephemerally wetted soils of the Antarctic Dry Valleys |
title_full_unstemmed |
Diverse and highly active diazotrophic assemblages inhabit ephemerally wetted soils of the Antarctic Dry Valleys |
title_sort |
diverse and highly active diazotrophic assemblages inhabit ephemerally wetted soils of the antarctic dry valleys |
publisher |
Blackwell Publishing Ltd |
publishDate |
2012 |
url |
https://hdl.handle.net/10289/6775 https://doi.org/10.1111/j.1574-6941.2012.01390.x |
op_coverage |
England |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
FEMS Microbiology Ecology Niederberger, T. D., Sohm, J. A., Tirindelli, J., Gunderson, T., Capone, D. G., Carpenter, E. J. and Cary, C.S. (2012), Diverse and highly active diazotrophic assemblages inhabit ephemerally wetted soils of the Antarctic Dry Valleys. FEMS Microbiology Ecology, 82(2), 376-390. 1574-6941 https://hdl.handle.net/10289/6775 doi:10.1111/j.1574-6941.2012.01390.x |
op_doi |
https://doi.org/10.1111/j.1574-6941.2012.01390.x |
container_title |
FEMS Microbiology Ecology |
container_volume |
82 |
container_issue |
2 |
container_start_page |
376 |
op_container_end_page |
390 |
_version_ |
1782327350094987264 |