Widespread distribution and identification of eight novel microcystins in Antarctic cyanobacterial mats
The microcystin content and cyanobacterial community structure of Antarctic microbial mat samples collected from 40 ponds, lakes and hydro-terrestrial environments were investigated. Samples were collected from Bratina Island and four of the Dry Valleys; Wrights, Victoria, Miers and Marshall. Enzyme...
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American Society for Microbiology
2008
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Online Access: | https://hdl.handle.net/10289/2830 https://doi.org/10.1128/AEM.01243-08 |
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ftunivwaikato:oai:researchcommons.waikato.ac.nz:10289/2830 2024-02-11T09:58:43+01:00 Widespread distribution and identification of eight novel microcystins in Antarctic cyanobacterial mats Wood, Susanna A. Mountfort, Douglas O. Selwood, Andrew I. Holland, Patrick T. Puddick, Jonathan Cary, S. Craig United States 2008 application/pdf https://hdl.handle.net/10289/2830 https://doi.org/10.1128/AEM.01243-08 en eng American Society for Microbiology http://aem.asm.org/cgi/content/abstract/74/23/7243 Applied and Environmental Microbiology Wood, S. A., Mountfort, D., Selwood, A. I., Holland, P. T., Puddick, J. & Cary, S. C. (2008). Widespread distribution and identification of eight novel microcystins in Antarctic cyanobacterial mats. Applied and Environmental Microbiology, 74(23), 7243-7251. https://hdl.handle.net/10289/2830 doi:10.1128/AEM.01243-08 This is an author’s accepted version of an article published in the journal: Applied and Environmental Microbiology. ©2008 American Society for Microbiology. biology Antarctic cyanobacterial mats Journal Article 2008 ftunivwaikato https://doi.org/10.1128/AEM.01243-08 2024-01-23T18:25:17Z The microcystin content and cyanobacterial community structure of Antarctic microbial mat samples collected from 40 ponds, lakes and hydro-terrestrial environments were investigated. Samples were collected from Bratina Island and four of the Dry Valleys; Wrights, Victoria, Miers and Marshall. Enzyme-linked immunosorbent assays (ELISA), liquid chromatography mass spectrometry (LC-MS), and protein phosphatase inhibition assays (PP-2A) resulted in the identification of low levels (1 - 16 mg/kg dry weight) of microcystins in all samples. A plot of indicative potencies of microcystins (ratio PP-2A:ELISA) versus total microcystins (ELISA) showed a general decrease in potency as total microcystin levels increased and a clustering of values from discrete geographic locations. LC-MS/MS analysis on selected samples identified eight novel microcystin congeners. The low energy collisional activation spectra were consistent with variants of [D-Asp3] MC-RR and [D-Asp3] MC-LR containing glycine [Gly1] rather than alanine and combinations of homoarginine [hAr2] or acetyldemethyl ADDA [ADMAdda5] substitutions. Nostoc sp. was identified as a microcystin producer using PCR amplification of a region of the 16S rRNA gene and the aminotransferase (AMT) domain of the mcyE gene. Automated ribosomal intergenic spacer analysis (ARISA) was undertaken to enable a comparison of cyanobacterial mat community structure from distant geographical locations. Two-dimensional multidimensional scaling ordination analysis of the ARISA data showed that in general samples from the same geographic location tended to clustered together. ARISA also enabled the putative identification of the microcystin producing Nostoc sp. from multiple samples. Article in Journal/Newspaper Antarc* Antarctic Bratina Island The University of Waikato: Research Commons Antarctic Miers ENVELOPE(163.850,163.850,-78.100,-78.100) Bratina Island ENVELOPE(165.533,165.533,-78.017,-78.017) Applied and Environmental Microbiology 74 23 7243 7251 |
institution |
Open Polar |
collection |
The University of Waikato: Research Commons |
op_collection_id |
ftunivwaikato |
language |
English |
topic |
biology Antarctic cyanobacterial mats |
spellingShingle |
biology Antarctic cyanobacterial mats Wood, Susanna A. Mountfort, Douglas O. Selwood, Andrew I. Holland, Patrick T. Puddick, Jonathan Cary, S. Craig Widespread distribution and identification of eight novel microcystins in Antarctic cyanobacterial mats |
topic_facet |
biology Antarctic cyanobacterial mats |
description |
The microcystin content and cyanobacterial community structure of Antarctic microbial mat samples collected from 40 ponds, lakes and hydro-terrestrial environments were investigated. Samples were collected from Bratina Island and four of the Dry Valleys; Wrights, Victoria, Miers and Marshall. Enzyme-linked immunosorbent assays (ELISA), liquid chromatography mass spectrometry (LC-MS), and protein phosphatase inhibition assays (PP-2A) resulted in the identification of low levels (1 - 16 mg/kg dry weight) of microcystins in all samples. A plot of indicative potencies of microcystins (ratio PP-2A:ELISA) versus total microcystins (ELISA) showed a general decrease in potency as total microcystin levels increased and a clustering of values from discrete geographic locations. LC-MS/MS analysis on selected samples identified eight novel microcystin congeners. The low energy collisional activation spectra were consistent with variants of [D-Asp3] MC-RR and [D-Asp3] MC-LR containing glycine [Gly1] rather than alanine and combinations of homoarginine [hAr2] or acetyldemethyl ADDA [ADMAdda5] substitutions. Nostoc sp. was identified as a microcystin producer using PCR amplification of a region of the 16S rRNA gene and the aminotransferase (AMT) domain of the mcyE gene. Automated ribosomal intergenic spacer analysis (ARISA) was undertaken to enable a comparison of cyanobacterial mat community structure from distant geographical locations. Two-dimensional multidimensional scaling ordination analysis of the ARISA data showed that in general samples from the same geographic location tended to clustered together. ARISA also enabled the putative identification of the microcystin producing Nostoc sp. from multiple samples. |
format |
Article in Journal/Newspaper |
author |
Wood, Susanna A. Mountfort, Douglas O. Selwood, Andrew I. Holland, Patrick T. Puddick, Jonathan Cary, S. Craig |
author_facet |
Wood, Susanna A. Mountfort, Douglas O. Selwood, Andrew I. Holland, Patrick T. Puddick, Jonathan Cary, S. Craig |
author_sort |
Wood, Susanna A. |
title |
Widespread distribution and identification of eight novel microcystins in Antarctic cyanobacterial mats |
title_short |
Widespread distribution and identification of eight novel microcystins in Antarctic cyanobacterial mats |
title_full |
Widespread distribution and identification of eight novel microcystins in Antarctic cyanobacterial mats |
title_fullStr |
Widespread distribution and identification of eight novel microcystins in Antarctic cyanobacterial mats |
title_full_unstemmed |
Widespread distribution and identification of eight novel microcystins in Antarctic cyanobacterial mats |
title_sort |
widespread distribution and identification of eight novel microcystins in antarctic cyanobacterial mats |
publisher |
American Society for Microbiology |
publishDate |
2008 |
url |
https://hdl.handle.net/10289/2830 https://doi.org/10.1128/AEM.01243-08 |
op_coverage |
United States |
long_lat |
ENVELOPE(163.850,163.850,-78.100,-78.100) ENVELOPE(165.533,165.533,-78.017,-78.017) |
geographic |
Antarctic Miers Bratina Island |
geographic_facet |
Antarctic Miers Bratina Island |
genre |
Antarc* Antarctic Bratina Island |
genre_facet |
Antarc* Antarctic Bratina Island |
op_relation |
http://aem.asm.org/cgi/content/abstract/74/23/7243 Applied and Environmental Microbiology Wood, S. A., Mountfort, D., Selwood, A. I., Holland, P. T., Puddick, J. & Cary, S. C. (2008). Widespread distribution and identification of eight novel microcystins in Antarctic cyanobacterial mats. Applied and Environmental Microbiology, 74(23), 7243-7251. https://hdl.handle.net/10289/2830 doi:10.1128/AEM.01243-08 |
op_rights |
This is an author’s accepted version of an article published in the journal: Applied and Environmental Microbiology. ©2008 American Society for Microbiology. |
op_doi |
https://doi.org/10.1128/AEM.01243-08 |
container_title |
Applied and Environmental Microbiology |
container_volume |
74 |
container_issue |
23 |
container_start_page |
7243 |
op_container_end_page |
7251 |
_version_ |
1790594460554362880 |