Phylogenetic analysis of actinobacterial populations associated with Antarctic Dry Valley mineral soils
Despite the apparent severity of the environmental conditions in the McMurdo Dry Valleys, Eastern Antarctica, recent phylogenetic studies conducted on mineral soil samples have revealed the presence of a wide diversity of microorganisms, with actinobacteria representing one of the largest phylotypic...
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Blackwell Publishing
2009
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Online Access: | https://hdl.handle.net/10289/2075 https://doi.org/10.1111/j.1462-2920.2008.01809.x |
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ftunivwaikato:oai:researchcommons.waikato.ac.nz:10289/2075 2024-01-21T10:01:35+01:00 Phylogenetic analysis of actinobacterial populations associated with Antarctic Dry Valley mineral soils Babalola, Olubukola Kirby, Bronwyn M. Roes-Hill, Marilize Le Cook, Andrew E. Cary, S. Craig Burton, Stephanie G. Cowan, Don A. England 2009 https://hdl.handle.net/10289/2075 https://doi.org/10.1111/j.1462-2920.2008.01809.x en eng Blackwell Publishing http://www3.interscience.wiley.com/journal/121509495/abstract Environmental Microbiology Babalola, O., Kirby, B.M., Roes-Hill, M. L. Cook, A.E., Cary, S.C. Burton, S.G. & Cowan, D.A. (2009). Phylogenetic analysis of actinobacterial populations associated with Antarctic Dry Valley mineral soils. Environmental Microbiology, 11(3), 566-576. https://hdl.handle.net/10289/2075 doi:10.1111/j.1462-2920.2008.01809.x phylogenetic analysis Journal Article 2009 ftunivwaikato https://doi.org/10.1111/j.1462-2920.2008.01809.x 2023-12-26T18:25:20Z Despite the apparent severity of the environmental conditions in the McMurdo Dry Valleys, Eastern Antarctica, recent phylogenetic studies conducted on mineral soil samples have revealed the presence of a wide diversity of microorganisms, with actinobacteria representing one of the largest phylotypic groups. Previous metagenomic studies have shown that the majority of Antarctic actinobacterial populations are classified as 'uncultured'. In this study, we assessed the diversity of actinobacteria in Antarctic cold desert soils by complementing traditional culture-based techniques with a metagenomic study. Phylogenetic analysis of clones generated with actinobacterium- and streptomycete-specific PCR primers revealed that the majority of the phylotypes were most closely related to uncultured Pseudonocardia and Nocardioides species. Phylotypes most closely related to a number of rarer actinobacteria genera, including Geodermatophilus, Modestobacter and Sporichthya, were also identified. While complementary culture-dependent studies isolated a number of Nocardia and Pseudonocardia species, the majority of the cultured isolates (> 80%) were Streptomyces species – although phylotypes affiliated to the genus Streptomyces were detected at a low frequency in the metagenomic study. This study confirms that Antarctic Dry Valley desert soil harbours highly diverse actinobacterial communities and suggests that many of the phylotypes identified may represent novel, uncultured species. Article in Journal/Newspaper Antarc* Antarctic Antarctica McMurdo Dry Valleys The University of Waikato: Research Commons Antarctic McMurdo Dry Valleys Environmental Microbiology 11 3 566 576 |
institution |
Open Polar |
collection |
The University of Waikato: Research Commons |
op_collection_id |
ftunivwaikato |
language |
English |
topic |
phylogenetic analysis |
spellingShingle |
phylogenetic analysis Babalola, Olubukola Kirby, Bronwyn M. Roes-Hill, Marilize Le Cook, Andrew E. Cary, S. Craig Burton, Stephanie G. Cowan, Don A. Phylogenetic analysis of actinobacterial populations associated with Antarctic Dry Valley mineral soils |
topic_facet |
phylogenetic analysis |
description |
Despite the apparent severity of the environmental conditions in the McMurdo Dry Valleys, Eastern Antarctica, recent phylogenetic studies conducted on mineral soil samples have revealed the presence of a wide diversity of microorganisms, with actinobacteria representing one of the largest phylotypic groups. Previous metagenomic studies have shown that the majority of Antarctic actinobacterial populations are classified as 'uncultured'. In this study, we assessed the diversity of actinobacteria in Antarctic cold desert soils by complementing traditional culture-based techniques with a metagenomic study. Phylogenetic analysis of clones generated with actinobacterium- and streptomycete-specific PCR primers revealed that the majority of the phylotypes were most closely related to uncultured Pseudonocardia and Nocardioides species. Phylotypes most closely related to a number of rarer actinobacteria genera, including Geodermatophilus, Modestobacter and Sporichthya, were also identified. While complementary culture-dependent studies isolated a number of Nocardia and Pseudonocardia species, the majority of the cultured isolates (> 80%) were Streptomyces species – although phylotypes affiliated to the genus Streptomyces were detected at a low frequency in the metagenomic study. This study confirms that Antarctic Dry Valley desert soil harbours highly diverse actinobacterial communities and suggests that many of the phylotypes identified may represent novel, uncultured species. |
format |
Article in Journal/Newspaper |
author |
Babalola, Olubukola Kirby, Bronwyn M. Roes-Hill, Marilize Le Cook, Andrew E. Cary, S. Craig Burton, Stephanie G. Cowan, Don A. |
author_facet |
Babalola, Olubukola Kirby, Bronwyn M. Roes-Hill, Marilize Le Cook, Andrew E. Cary, S. Craig Burton, Stephanie G. Cowan, Don A. |
author_sort |
Babalola, Olubukola |
title |
Phylogenetic analysis of actinobacterial populations associated with Antarctic Dry Valley mineral soils |
title_short |
Phylogenetic analysis of actinobacterial populations associated with Antarctic Dry Valley mineral soils |
title_full |
Phylogenetic analysis of actinobacterial populations associated with Antarctic Dry Valley mineral soils |
title_fullStr |
Phylogenetic analysis of actinobacterial populations associated with Antarctic Dry Valley mineral soils |
title_full_unstemmed |
Phylogenetic analysis of actinobacterial populations associated with Antarctic Dry Valley mineral soils |
title_sort |
phylogenetic analysis of actinobacterial populations associated with antarctic dry valley mineral soils |
publisher |
Blackwell Publishing |
publishDate |
2009 |
url |
https://hdl.handle.net/10289/2075 https://doi.org/10.1111/j.1462-2920.2008.01809.x |
op_coverage |
England |
geographic |
Antarctic McMurdo Dry Valleys |
geographic_facet |
Antarctic McMurdo Dry Valleys |
genre |
Antarc* Antarctic Antarctica McMurdo Dry Valleys |
genre_facet |
Antarc* Antarctic Antarctica McMurdo Dry Valleys |
op_relation |
http://www3.interscience.wiley.com/journal/121509495/abstract Environmental Microbiology Babalola, O., Kirby, B.M., Roes-Hill, M. L. Cook, A.E., Cary, S.C. Burton, S.G. & Cowan, D.A. (2009). Phylogenetic analysis of actinobacterial populations associated with Antarctic Dry Valley mineral soils. Environmental Microbiology, 11(3), 566-576. https://hdl.handle.net/10289/2075 doi:10.1111/j.1462-2920.2008.01809.x |
op_doi |
https://doi.org/10.1111/j.1462-2920.2008.01809.x |
container_title |
Environmental Microbiology |
container_volume |
11 |
container_issue |
3 |
container_start_page |
566 |
op_container_end_page |
576 |
_version_ |
1788691584991100928 |