Phylogeny and lipid profiles of snow-algae isolated from Norwegian red-snow microbiomes
Snow algae blooms often form green or red coloured patches in melting alpine and polar snowfields worldwide, yet little is known about their biology, biogeography, and species diversity. We investigated eight isolates collected from red snow in northern Norway, using a combination of morphology, 18S...
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ftunivwagenin:oai:library.wur.nl:wurpubs/620163 2024-04-28T08:32:29+00:00 Phylogeny and lipid profiles of snow-algae isolated from Norwegian red-snow microbiomes Suzuki, Hirono Dtain, Alexandre Park, Youngjin Viswanath, Kiron Wijffels, Ren H. Leborgne-Castel, Nathalie Procházková, Lenka Hulatt, Chris J. 2023 application/pdf https://research.wur.nl/en/publications/phylogeny-and-lipid-profiles-of-snow-algae-isolated-from-norwegia https://doi.org/10.1093/femsec/fiad057 en eng https://edepot.wur.nl/640322 https://research.wur.nl/en/publications/phylogeny-and-lipid-profiles-of-snow-algae-isolated-from-norwegia doi:10.1093/femsec/fiad057 https://creativecommons.org/licenses/by/4.0/ Wageningen University & Research FEMS Microbiology Ecology 99 (2023) 6 ISSN: 0168-6496 18S rRNA ITS2 rRNA fatty acids imaging flow cytometry microalgae phylogeny Article/Letter to editor 2023 ftunivwagenin https://doi.org/10.1093/femsec/fiad057 2024-04-03T14:26:15Z Snow algae blooms often form green or red coloured patches in melting alpine and polar snowfields worldwide, yet little is known about their biology, biogeography, and species diversity. We investigated eight isolates collected from red snow in northern Norway, using a combination of morphology, 18S rRNA gene and internal transcribed spacer 2 (ITS2) genetic markers. Phylogenetic and ITS2 rRNA secondary structure analyses assigned six isolates to the species Raphidonema nivale, Deuterostichococcus epilithicus, Chloromonas reticulata, and Xanthonema bristolianum. Two novel isolates belonging to the family Stichococcaceae (ARK-S05-19) and the genus Chloromonas (ARK-S08-19) were identified as potentially new species. In laboratory cultivation, differences in the growth rate and fatty acid profiles were observed between the strains. Chlorophyta were characterized by abundant C18:3n-3 fatty-acids with increases in C18:1n-9 in the stationary phase, whilst Xanthonema (Ochrophyta) was characterized by a large proportion of C20:5n-3, with increases in C16:1n-7 in the stationary phase. In a further experiment, lipid droplet formation was studied in C. reticulata at the single-cell level using imaging flow cytometry. Our study establishes new cultures of snow algae, reveals novel data on their biodiversity and biogeography, and provides an initial characterization of physiological traits that shape natural communities and their ecophysiological properties. Article in Journal/Newspaper Northern Norway Wageningen UR (University & Research Centre): Digital Library FEMS Microbiology Ecology 99 6 |
institution |
Open Polar |
collection |
Wageningen UR (University & Research Centre): Digital Library |
op_collection_id |
ftunivwagenin |
language |
English |
topic |
18S rRNA ITS2 rRNA fatty acids imaging flow cytometry microalgae phylogeny |
spellingShingle |
18S rRNA ITS2 rRNA fatty acids imaging flow cytometry microalgae phylogeny Suzuki, Hirono Dtain, Alexandre Park, Youngjin Viswanath, Kiron Wijffels, Ren H. Leborgne-Castel, Nathalie Procházková, Lenka Hulatt, Chris J. Phylogeny and lipid profiles of snow-algae isolated from Norwegian red-snow microbiomes |
topic_facet |
18S rRNA ITS2 rRNA fatty acids imaging flow cytometry microalgae phylogeny |
description |
Snow algae blooms often form green or red coloured patches in melting alpine and polar snowfields worldwide, yet little is known about their biology, biogeography, and species diversity. We investigated eight isolates collected from red snow in northern Norway, using a combination of morphology, 18S rRNA gene and internal transcribed spacer 2 (ITS2) genetic markers. Phylogenetic and ITS2 rRNA secondary structure analyses assigned six isolates to the species Raphidonema nivale, Deuterostichococcus epilithicus, Chloromonas reticulata, and Xanthonema bristolianum. Two novel isolates belonging to the family Stichococcaceae (ARK-S05-19) and the genus Chloromonas (ARK-S08-19) were identified as potentially new species. In laboratory cultivation, differences in the growth rate and fatty acid profiles were observed between the strains. Chlorophyta were characterized by abundant C18:3n-3 fatty-acids with increases in C18:1n-9 in the stationary phase, whilst Xanthonema (Ochrophyta) was characterized by a large proportion of C20:5n-3, with increases in C16:1n-7 in the stationary phase. In a further experiment, lipid droplet formation was studied in C. reticulata at the single-cell level using imaging flow cytometry. Our study establishes new cultures of snow algae, reveals novel data on their biodiversity and biogeography, and provides an initial characterization of physiological traits that shape natural communities and their ecophysiological properties. |
format |
Article in Journal/Newspaper |
author |
Suzuki, Hirono Dtain, Alexandre Park, Youngjin Viswanath, Kiron Wijffels, Ren H. Leborgne-Castel, Nathalie Procházková, Lenka Hulatt, Chris J. |
author_facet |
Suzuki, Hirono Dtain, Alexandre Park, Youngjin Viswanath, Kiron Wijffels, Ren H. Leborgne-Castel, Nathalie Procházková, Lenka Hulatt, Chris J. |
author_sort |
Suzuki, Hirono |
title |
Phylogeny and lipid profiles of snow-algae isolated from Norwegian red-snow microbiomes |
title_short |
Phylogeny and lipid profiles of snow-algae isolated from Norwegian red-snow microbiomes |
title_full |
Phylogeny and lipid profiles of snow-algae isolated from Norwegian red-snow microbiomes |
title_fullStr |
Phylogeny and lipid profiles of snow-algae isolated from Norwegian red-snow microbiomes |
title_full_unstemmed |
Phylogeny and lipid profiles of snow-algae isolated from Norwegian red-snow microbiomes |
title_sort |
phylogeny and lipid profiles of snow-algae isolated from norwegian red-snow microbiomes |
publishDate |
2023 |
url |
https://research.wur.nl/en/publications/phylogeny-and-lipid-profiles-of-snow-algae-isolated-from-norwegia https://doi.org/10.1093/femsec/fiad057 |
genre |
Northern Norway |
genre_facet |
Northern Norway |
op_source |
FEMS Microbiology Ecology 99 (2023) 6 ISSN: 0168-6496 |
op_relation |
https://edepot.wur.nl/640322 https://research.wur.nl/en/publications/phylogeny-and-lipid-profiles-of-snow-algae-isolated-from-norwegia doi:10.1093/femsec/fiad057 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ Wageningen University & Research |
op_doi |
https://doi.org/10.1093/femsec/fiad057 |
container_title |
FEMS Microbiology Ecology |
container_volume |
99 |
container_issue |
6 |
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1797589662027481088 |