Application of the two-sample doubly labelled water method alters behaviour and affects estimates of energy expenditure in black-legged kittiwakes
Despite the widespread use of the doubly labelled water (DLW) method in energetic studies of free-ranging animals, effects of the method on study animals are rarely assessed. We studied behavioural effects of two alternative DLW protocols. During two consecutive breeding seasons, 42 parent black-leg...
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ftunivwagenin:oai:library.wur.nl:wurpubs/588904 2024-02-04T10:04:13+01:00 Application of the two-sample doubly labelled water method alters behaviour and affects estimates of energy expenditure in black-legged kittiwakes Schultner, Jannik Welcker, Jörg Speakman, John R. Nordoy, Erling S. Gabrielsen, Geir W. 2010 text/html https://research.wur.nl/en/publications/application-of-the-two-sample-doubly-labelled-water-method-alters https://doi.org/10.1242/jeb.043414 en eng https://edepot.wur.nl/556455 https://research.wur.nl/en/publications/application-of-the-two-sample-doubly-labelled-water-method-alters doi:10.1242/jeb.043414 info:eu-repo/semantics/restrictedAccess Wageningen University & Research Journal of Experimental Biology 213 (2010) 17 ISSN: 0022-0949 Behavioural effect DLW Field metabolic rate Nest attendance Rissa tridactyla Single sample info:eu-repo/semantics/article Article/Letter to editor info:eu-repo/semantics/publishedVersion 2010 ftunivwagenin https://doi.org/10.1242/jeb.043414 2024-01-10T23:22:39Z Despite the widespread use of the doubly labelled water (DLW) method in energetic studies of free-ranging animals, effects of the method on study animals are rarely assessed. We studied behavioural effects of two alternative DLW protocols. During two consecutive breeding seasons, 42 parent black-legged kittiwakes received either the commonly used two-sample (TS) or the less invasive single-sample (SS) DLW treatment. A third group served as a non-treated control. We evaluated the effect of treatment with respect to the time birds took to return to their nest after treatment and recaptures, and the nest attendance during DLW measurement periods. We found that TS kittiwakes took on average 20 times longer to return to their nest than SS kittiwakes after initial treatment, and nest attendance was reduced by about 40% relative to control birds. In contrast, nest attendance did not differ between control and SS kittiwakes. Estimates of energy expenditure of SS kittiwakes exceeded those of TS kittiwakes by 15%. This difference was probably caused by TS birds remaining inactive for extended time periods while at sea. Our results demonstrate that the common assumption that the TS DLW method has little impact on the behaviour of study subjects is in some circumstances fallacious. Estimates of energy expenditure derived by the SS approach may thus more accurately reflect unbiased rates of energy expenditure. However, the choice of protocol may be a trade-off between their impact on behaviour, and hence accuracy, and their differences in precision. Adopting procedures that minimize the impact of TS protocols may be useful. Article in Journal/Newspaper rissa tridactyla Wageningen UR (University & Research Centre): Digital Library Journal of Experimental Biology 213 17 2958 2966 |
institution |
Open Polar |
collection |
Wageningen UR (University & Research Centre): Digital Library |
op_collection_id |
ftunivwagenin |
language |
English |
topic |
Behavioural effect DLW Field metabolic rate Nest attendance Rissa tridactyla Single sample |
spellingShingle |
Behavioural effect DLW Field metabolic rate Nest attendance Rissa tridactyla Single sample Schultner, Jannik Welcker, Jörg Speakman, John R. Nordoy, Erling S. Gabrielsen, Geir W. Application of the two-sample doubly labelled water method alters behaviour and affects estimates of energy expenditure in black-legged kittiwakes |
topic_facet |
Behavioural effect DLW Field metabolic rate Nest attendance Rissa tridactyla Single sample |
description |
Despite the widespread use of the doubly labelled water (DLW) method in energetic studies of free-ranging animals, effects of the method on study animals are rarely assessed. We studied behavioural effects of two alternative DLW protocols. During two consecutive breeding seasons, 42 parent black-legged kittiwakes received either the commonly used two-sample (TS) or the less invasive single-sample (SS) DLW treatment. A third group served as a non-treated control. We evaluated the effect of treatment with respect to the time birds took to return to their nest after treatment and recaptures, and the nest attendance during DLW measurement periods. We found that TS kittiwakes took on average 20 times longer to return to their nest than SS kittiwakes after initial treatment, and nest attendance was reduced by about 40% relative to control birds. In contrast, nest attendance did not differ between control and SS kittiwakes. Estimates of energy expenditure of SS kittiwakes exceeded those of TS kittiwakes by 15%. This difference was probably caused by TS birds remaining inactive for extended time periods while at sea. Our results demonstrate that the common assumption that the TS DLW method has little impact on the behaviour of study subjects is in some circumstances fallacious. Estimates of energy expenditure derived by the SS approach may thus more accurately reflect unbiased rates of energy expenditure. However, the choice of protocol may be a trade-off between their impact on behaviour, and hence accuracy, and their differences in precision. Adopting procedures that minimize the impact of TS protocols may be useful. |
format |
Article in Journal/Newspaper |
author |
Schultner, Jannik Welcker, Jörg Speakman, John R. Nordoy, Erling S. Gabrielsen, Geir W. |
author_facet |
Schultner, Jannik Welcker, Jörg Speakman, John R. Nordoy, Erling S. Gabrielsen, Geir W. |
author_sort |
Schultner, Jannik |
title |
Application of the two-sample doubly labelled water method alters behaviour and affects estimates of energy expenditure in black-legged kittiwakes |
title_short |
Application of the two-sample doubly labelled water method alters behaviour and affects estimates of energy expenditure in black-legged kittiwakes |
title_full |
Application of the two-sample doubly labelled water method alters behaviour and affects estimates of energy expenditure in black-legged kittiwakes |
title_fullStr |
Application of the two-sample doubly labelled water method alters behaviour and affects estimates of energy expenditure in black-legged kittiwakes |
title_full_unstemmed |
Application of the two-sample doubly labelled water method alters behaviour and affects estimates of energy expenditure in black-legged kittiwakes |
title_sort |
application of the two-sample doubly labelled water method alters behaviour and affects estimates of energy expenditure in black-legged kittiwakes |
publishDate |
2010 |
url |
https://research.wur.nl/en/publications/application-of-the-two-sample-doubly-labelled-water-method-alters https://doi.org/10.1242/jeb.043414 |
genre |
rissa tridactyla |
genre_facet |
rissa tridactyla |
op_source |
Journal of Experimental Biology 213 (2010) 17 ISSN: 0022-0949 |
op_relation |
https://edepot.wur.nl/556455 https://research.wur.nl/en/publications/application-of-the-two-sample-doubly-labelled-water-method-alters doi:10.1242/jeb.043414 |
op_rights |
info:eu-repo/semantics/restrictedAccess Wageningen University & Research |
op_doi |
https://doi.org/10.1242/jeb.043414 |
container_title |
Journal of Experimental Biology |
container_volume |
213 |
container_issue |
17 |
container_start_page |
2958 |
op_container_end_page |
2966 |
_version_ |
1789972300681969664 |