Different lipid forms of omega-3 and their effect on small intestine in mice
Omega - 3 fatty acids of marine origin exert beneficial effects on lipid metabolism and can protect against insulin resistance in high fat diet (HFD)-fed animals. Simultaneously, recent studies showed that different lipid forms could have numerous consequences regarding the regulation of energy bala...
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Wageningen University
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ftunivwagenin:oai:library.wur.nl:wurpubs/567929 2024-02-04T09:59:24+01:00 Different lipid forms of omega-3 and their effect on small intestine in mice van Schothorst, Evert Kucharikova, P. Bunschoten, Annelies Horakova, Olga Rossmeisl, Martin Kopecky, Jan Keijer, Jaap 2020 text/html https://research.wur.nl/en/datasets/different-lipid-forms-of-omega-3-and-their-effect-on-small-intest unknown Wageningen University https://edepot.wur.nl/527695 https://research.wur.nl/en/datasets/different-lipid-forms-of-omega-3-and-their-effect-on-small-intest info:eu-repo/semantics/openAccess Wageningen University & Research Mus musculus info:eu-repo/semantics/other info:eu-repo/semantics/publishedVersion 2020 ftunivwagenin 2024-01-10T23:15:35Z Omega - 3 fatty acids of marine origin exert beneficial effects on lipid metabolism and can protect against insulin resistance in high fat diet (HFD)-fed animals. Simultaneously, recent studies showed that different lipid forms could have numerous consequences regarding the regulation of energy balance, nutrient absorption, and substrate metabolism. Indeed, when omega-3 was provided as triglycerides (TG, i.e. fish oil), it induced dose-dependently the expression of genes involved in lipid metabolism as well as fatty acid oxidation in small intestine of C57BL/6 mice fed various HFDs. As the underlying mechanism(s) explaining the differences in EPA/DHA bioavailability among various lipid forms of Omega-3 is not entirely clear, we performed a mouse study (n=8 per group) using purified HFDs with control HFD based on corn oil (cHF) and part of the lipids were replaced by omega-3 fish lipids in different forms: as either TG (cHF-F), marine phospholipids (PL; Krill oil, given at two different doses Krill-low (Krill-L) and Krill-high (Krill-H)), and as wax esters in the extract from the zooplankton Calanus finmarchicus (Calanus oil CAL-L representing same omega-3 levels as Krill-L diet). As a healthy control we fed a subset of mice standard chow (STD). All mice were fed their diet for 8 weeks and after sacrifice, whole small intestine was isolated, frozen and used for RNA isolation and microarray gene expression analysis using 8x60K Agilent arrays. Results showed that PL-H versus control cHFc induced specifically metabolic lipid pathways, while TG and PL-L mainly affected cytoskeleton regulation. Other/Unknown Material Calanus finmarchicus Wageningen UR (University & Research Centre): Digital Library |
institution |
Open Polar |
collection |
Wageningen UR (University & Research Centre): Digital Library |
op_collection_id |
ftunivwagenin |
language |
unknown |
topic |
Mus musculus |
spellingShingle |
Mus musculus van Schothorst, Evert Kucharikova, P. Bunschoten, Annelies Horakova, Olga Rossmeisl, Martin Kopecky, Jan Keijer, Jaap Different lipid forms of omega-3 and their effect on small intestine in mice |
topic_facet |
Mus musculus |
description |
Omega - 3 fatty acids of marine origin exert beneficial effects on lipid metabolism and can protect against insulin resistance in high fat diet (HFD)-fed animals. Simultaneously, recent studies showed that different lipid forms could have numerous consequences regarding the regulation of energy balance, nutrient absorption, and substrate metabolism. Indeed, when omega-3 was provided as triglycerides (TG, i.e. fish oil), it induced dose-dependently the expression of genes involved in lipid metabolism as well as fatty acid oxidation in small intestine of C57BL/6 mice fed various HFDs. As the underlying mechanism(s) explaining the differences in EPA/DHA bioavailability among various lipid forms of Omega-3 is not entirely clear, we performed a mouse study (n=8 per group) using purified HFDs with control HFD based on corn oil (cHF) and part of the lipids were replaced by omega-3 fish lipids in different forms: as either TG (cHF-F), marine phospholipids (PL; Krill oil, given at two different doses Krill-low (Krill-L) and Krill-high (Krill-H)), and as wax esters in the extract from the zooplankton Calanus finmarchicus (Calanus oil CAL-L representing same omega-3 levels as Krill-L diet). As a healthy control we fed a subset of mice standard chow (STD). All mice were fed their diet for 8 weeks and after sacrifice, whole small intestine was isolated, frozen and used for RNA isolation and microarray gene expression analysis using 8x60K Agilent arrays. Results showed that PL-H versus control cHFc induced specifically metabolic lipid pathways, while TG and PL-L mainly affected cytoskeleton regulation. |
format |
Other/Unknown Material |
author |
van Schothorst, Evert Kucharikova, P. Bunschoten, Annelies Horakova, Olga Rossmeisl, Martin Kopecky, Jan Keijer, Jaap |
author_facet |
van Schothorst, Evert Kucharikova, P. Bunschoten, Annelies Horakova, Olga Rossmeisl, Martin Kopecky, Jan Keijer, Jaap |
author_sort |
van Schothorst, Evert |
title |
Different lipid forms of omega-3 and their effect on small intestine in mice |
title_short |
Different lipid forms of omega-3 and their effect on small intestine in mice |
title_full |
Different lipid forms of omega-3 and their effect on small intestine in mice |
title_fullStr |
Different lipid forms of omega-3 and their effect on small intestine in mice |
title_full_unstemmed |
Different lipid forms of omega-3 and their effect on small intestine in mice |
title_sort |
different lipid forms of omega-3 and their effect on small intestine in mice |
publisher |
Wageningen University |
publishDate |
2020 |
url |
https://research.wur.nl/en/datasets/different-lipid-forms-of-omega-3-and-their-effect-on-small-intest |
genre |
Calanus finmarchicus |
genre_facet |
Calanus finmarchicus |
op_relation |
https://edepot.wur.nl/527695 https://research.wur.nl/en/datasets/different-lipid-forms-of-omega-3-and-their-effect-on-small-intest |
op_rights |
info:eu-repo/semantics/openAccess Wageningen University & Research |
_version_ |
1789964188091678720 |