Mitogen and antigen induced B and T cell responses of peripheral blood mononuclear cells from the harbour seal (Phoca vitulina)
In vitro assays were developed for studies concerning the functioning of the immune system of the harbour seal (Phoca vitulina). Proliferative responses of peripheral blood mononuclear cells (PBMC) were measured after stimulation with different concentrations of the mitogens concanavalin A (Con A),...
Published in: | Veterinary Immunology and Immunopathology |
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1993
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ftunivwagenin:oai:library.wur.nl:wurpubs/556623 2024-02-04T10:01:01+01:00 Mitogen and antigen induced B and T cell responses of peripheral blood mononuclear cells from the harbour seal (Phoca vitulina) de Swart, Rik L. Kluten, Ruud M.G. Huizing, Carla J. Vedder, Lies J. Reijnders, Peter J.H. Visser, Ilona K.G. Uytdehaag, Fons G.C.M. Osterhaus, Ab D.M.E. 1993 application/pdf https://research.wur.nl/en/publications/mitogen-and-antigen-induced-b-and-t-cell-responses-of-peripheral- https://doi.org/10.1016/0165-2427(93)90195-A en eng https://edepot.wur.nl/508180 https://research.wur.nl/en/publications/mitogen-and-antigen-induced-b-and-t-cell-responses-of-peripheral- doi:10.1016/0165-2427(93)90195-A info:eu-repo/semantics/restrictedAccess Wageningen University & Research Veterinary Immunology and Immunopathology 37 (1993) 3-4 ISSN: 0165-2427 Life Science info:eu-repo/semantics/article Article/Letter to editor info:eu-repo/semantics/publishedVersion 1993 ftunivwagenin https://doi.org/10.1016/0165-2427(93)90195-A 2024-01-10T23:30:01Z In vitro assays were developed for studies concerning the functioning of the immune system of the harbour seal (Phoca vitulina). Proliferative responses of peripheral blood mononuclear cells (PBMC) were measured after stimulation with different concentrations of the mitogens concanavalin A (Con A), pokeweed mitogen (PWM), phytohaemagglutinin (PHA) or lipopolysaccharide from Salmonella typhimurium (LPS). Con A and PWM induced strong proliferative responses, while PHA and LPS induced comparatively low proliferative responses. Responses of mitogen stimulated PBMC to recombinant human interleukin-2 (rhIL-2) and in vitro immunoglobulin production by mitogen stimulated PBMC were measured to discriminate between stimulation of T cells and B cells. It was found that Con A and PHA stimulate phocine T cells, PWM stimulates both T cells and B cells and LPS predominantly stimulates phocine B cells. Antigen-specific immune responses were measured after immunization of seals with an inactivated rabies vaccine and/or with tetanus toxoid. Antigen-specific proliferation of PBMC and the presence of antigen-specific antibody forming cells were demonstrated for both antigens in the PBMC of immunized animals. The responses measured in vitro correlated well with the development of specific serum antibody titers to these antigens. Article in Journal/Newspaper harbour seal Phoca vitulina Wageningen UR (University & Research Centre): Digital Library Veterinary Immunology and Immunopathology 37 3-4 217 230 |
institution |
Open Polar |
collection |
Wageningen UR (University & Research Centre): Digital Library |
op_collection_id |
ftunivwagenin |
language |
English |
topic |
Life Science |
spellingShingle |
Life Science de Swart, Rik L. Kluten, Ruud M.G. Huizing, Carla J. Vedder, Lies J. Reijnders, Peter J.H. Visser, Ilona K.G. Uytdehaag, Fons G.C.M. Osterhaus, Ab D.M.E. Mitogen and antigen induced B and T cell responses of peripheral blood mononuclear cells from the harbour seal (Phoca vitulina) |
topic_facet |
Life Science |
description |
In vitro assays were developed for studies concerning the functioning of the immune system of the harbour seal (Phoca vitulina). Proliferative responses of peripheral blood mononuclear cells (PBMC) were measured after stimulation with different concentrations of the mitogens concanavalin A (Con A), pokeweed mitogen (PWM), phytohaemagglutinin (PHA) or lipopolysaccharide from Salmonella typhimurium (LPS). Con A and PWM induced strong proliferative responses, while PHA and LPS induced comparatively low proliferative responses. Responses of mitogen stimulated PBMC to recombinant human interleukin-2 (rhIL-2) and in vitro immunoglobulin production by mitogen stimulated PBMC were measured to discriminate between stimulation of T cells and B cells. It was found that Con A and PHA stimulate phocine T cells, PWM stimulates both T cells and B cells and LPS predominantly stimulates phocine B cells. Antigen-specific immune responses were measured after immunization of seals with an inactivated rabies vaccine and/or with tetanus toxoid. Antigen-specific proliferation of PBMC and the presence of antigen-specific antibody forming cells were demonstrated for both antigens in the PBMC of immunized animals. The responses measured in vitro correlated well with the development of specific serum antibody titers to these antigens. |
format |
Article in Journal/Newspaper |
author |
de Swart, Rik L. Kluten, Ruud M.G. Huizing, Carla J. Vedder, Lies J. Reijnders, Peter J.H. Visser, Ilona K.G. Uytdehaag, Fons G.C.M. Osterhaus, Ab D.M.E. |
author_facet |
de Swart, Rik L. Kluten, Ruud M.G. Huizing, Carla J. Vedder, Lies J. Reijnders, Peter J.H. Visser, Ilona K.G. Uytdehaag, Fons G.C.M. Osterhaus, Ab D.M.E. |
author_sort |
de Swart, Rik L. |
title |
Mitogen and antigen induced B and T cell responses of peripheral blood mononuclear cells from the harbour seal (Phoca vitulina) |
title_short |
Mitogen and antigen induced B and T cell responses of peripheral blood mononuclear cells from the harbour seal (Phoca vitulina) |
title_full |
Mitogen and antigen induced B and T cell responses of peripheral blood mononuclear cells from the harbour seal (Phoca vitulina) |
title_fullStr |
Mitogen and antigen induced B and T cell responses of peripheral blood mononuclear cells from the harbour seal (Phoca vitulina) |
title_full_unstemmed |
Mitogen and antigen induced B and T cell responses of peripheral blood mononuclear cells from the harbour seal (Phoca vitulina) |
title_sort |
mitogen and antigen induced b and t cell responses of peripheral blood mononuclear cells from the harbour seal (phoca vitulina) |
publishDate |
1993 |
url |
https://research.wur.nl/en/publications/mitogen-and-antigen-induced-b-and-t-cell-responses-of-peripheral- https://doi.org/10.1016/0165-2427(93)90195-A |
genre |
harbour seal Phoca vitulina |
genre_facet |
harbour seal Phoca vitulina |
op_source |
Veterinary Immunology and Immunopathology 37 (1993) 3-4 ISSN: 0165-2427 |
op_relation |
https://edepot.wur.nl/508180 https://research.wur.nl/en/publications/mitogen-and-antigen-induced-b-and-t-cell-responses-of-peripheral- doi:10.1016/0165-2427(93)90195-A |
op_rights |
info:eu-repo/semantics/restrictedAccess Wageningen University & Research |
op_doi |
https://doi.org/10.1016/0165-2427(93)90195-A |
container_title |
Veterinary Immunology and Immunopathology |
container_volume |
37 |
container_issue |
3-4 |
container_start_page |
217 |
op_container_end_page |
230 |
_version_ |
1789966625534902272 |