Fatty acids and proteins from marine cold adapted microalgae for biotechnology
Cold-adapted microalgae display unexpectedly high biomass production, pointing to their potential to produce high-value bioproducts under cold and light-limited conditions. From culture collections, we screened eight cold-adapted strains of different genera (Chlamydomonas, Chlorella, Tetraselmis, Ps...
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ftunivwagenin:oai:library.wur.nl:wurpubs/552696 2024-01-14T10:00:07+01:00 Fatty acids and proteins from marine cold adapted microalgae for biotechnology Schulze, Peter S.C. Hulatt, Christopher J. Morales-Sánchez, Daniela Wijffels, René H. Kiron, Viswanath 2019 application/pdf https://research.wur.nl/en/publications/fatty-acids-and-proteins-from-marine-cold-adapted-microalgae-for- https://doi.org/10.1016/j.algal.2019.101604 en eng https://edepot.wur.nl/497963 https://research.wur.nl/en/publications/fatty-acids-and-proteins-from-marine-cold-adapted-microalgae-for- doi:10.1016/j.algal.2019.101604 info:eu-repo/semantics/restrictedAccess Wageningen University & Research Algal Research 42 (2019) ISSN: 2211-9264 Cold water algae Eicosapentaenoic acid (EPA) Light Polyunsaturated fatty acid (PUFA) Protein Temperature info:eu-repo/semantics/article Article/Letter to editor info:eu-repo/semantics/publishedVersion 2019 ftunivwagenin https://doi.org/10.1016/j.algal.2019.101604 2023-12-20T23:15:54Z Cold-adapted microalgae display unexpectedly high biomass production, pointing to their potential to produce high-value bioproducts under cold and light-limited conditions. From culture collections, we screened eight cold-adapted strains of different genera (Chlamydomonas, Chlorella, Tetraselmis, Pseudopleurochloris, Nannochloropsis and Phaeodactylum) for the production of fatty acids and proteins under low temperature and light regimes (T = 8, 15 °C; I = 50, 100 μmol s−1 m−2). Among the strains, the Arctic isolate Chlamydomonas sp. (RCC 2488) had better growth at 8 °C compared to 15 °C (up to 0.5 gDW L−1 d−1) and highest productivities of protein and polyunsaturated fatty acids (PUFA) (70 and 65 mg L−1 d−1, respectively). Two tested Tetraselmis strains (SAG 1.96, RCC 2604) achieved highest biomass productivities (0.7–1 gDW L−1 d−1), containing up to 50 mg PUFA gDW−1 and 15% proteins. Pseudopleurochloris antarctica (SAG 39.98) grew well at 15 °C (0.4 g L−1 d−1), with 23% proteins in biomass and the highest eicosapentaenoic acid (EPA) productivity (7.6 mg L−1 d−1). Chlorella stigmatophora (RCC 661) achieved productivities of 0.4 gDW L−1 d−1 at 15 °C and produced extracellular polymeric substances (EPS). The major cause for the observed shifts in biochemical profiles was biomass concentration, which is an indicator for the prevailing growth stage. Based on the current experimental design, Chlamydomonas sp. (RCC 2488), T. chuii and P. antarctica can be suggested as the most promising strains for the production of protein and (polyunsaturated-) fatty acids at low temperatures. However, additional strain-specific studies are necessary to statistically validate these findings. Article in Journal/Newspaper Antarc* Antarctica Arctic Wageningen UR (University & Research Centre): Digital Library Arctic Algal Research 42 101604 |
institution |
Open Polar |
collection |
Wageningen UR (University & Research Centre): Digital Library |
op_collection_id |
ftunivwagenin |
language |
English |
topic |
Cold water algae Eicosapentaenoic acid (EPA) Light Polyunsaturated fatty acid (PUFA) Protein Temperature |
spellingShingle |
Cold water algae Eicosapentaenoic acid (EPA) Light Polyunsaturated fatty acid (PUFA) Protein Temperature Schulze, Peter S.C. Hulatt, Christopher J. Morales-Sánchez, Daniela Wijffels, René H. Kiron, Viswanath Fatty acids and proteins from marine cold adapted microalgae for biotechnology |
topic_facet |
Cold water algae Eicosapentaenoic acid (EPA) Light Polyunsaturated fatty acid (PUFA) Protein Temperature |
description |
Cold-adapted microalgae display unexpectedly high biomass production, pointing to their potential to produce high-value bioproducts under cold and light-limited conditions. From culture collections, we screened eight cold-adapted strains of different genera (Chlamydomonas, Chlorella, Tetraselmis, Pseudopleurochloris, Nannochloropsis and Phaeodactylum) for the production of fatty acids and proteins under low temperature and light regimes (T = 8, 15 °C; I = 50, 100 μmol s−1 m−2). Among the strains, the Arctic isolate Chlamydomonas sp. (RCC 2488) had better growth at 8 °C compared to 15 °C (up to 0.5 gDW L−1 d−1) and highest productivities of protein and polyunsaturated fatty acids (PUFA) (70 and 65 mg L−1 d−1, respectively). Two tested Tetraselmis strains (SAG 1.96, RCC 2604) achieved highest biomass productivities (0.7–1 gDW L−1 d−1), containing up to 50 mg PUFA gDW−1 and 15% proteins. Pseudopleurochloris antarctica (SAG 39.98) grew well at 15 °C (0.4 g L−1 d−1), with 23% proteins in biomass and the highest eicosapentaenoic acid (EPA) productivity (7.6 mg L−1 d−1). Chlorella stigmatophora (RCC 661) achieved productivities of 0.4 gDW L−1 d−1 at 15 °C and produced extracellular polymeric substances (EPS). The major cause for the observed shifts in biochemical profiles was biomass concentration, which is an indicator for the prevailing growth stage. Based on the current experimental design, Chlamydomonas sp. (RCC 2488), T. chuii and P. antarctica can be suggested as the most promising strains for the production of protein and (polyunsaturated-) fatty acids at low temperatures. However, additional strain-specific studies are necessary to statistically validate these findings. |
format |
Article in Journal/Newspaper |
author |
Schulze, Peter S.C. Hulatt, Christopher J. Morales-Sánchez, Daniela Wijffels, René H. Kiron, Viswanath |
author_facet |
Schulze, Peter S.C. Hulatt, Christopher J. Morales-Sánchez, Daniela Wijffels, René H. Kiron, Viswanath |
author_sort |
Schulze, Peter S.C. |
title |
Fatty acids and proteins from marine cold adapted microalgae for biotechnology |
title_short |
Fatty acids and proteins from marine cold adapted microalgae for biotechnology |
title_full |
Fatty acids and proteins from marine cold adapted microalgae for biotechnology |
title_fullStr |
Fatty acids and proteins from marine cold adapted microalgae for biotechnology |
title_full_unstemmed |
Fatty acids and proteins from marine cold adapted microalgae for biotechnology |
title_sort |
fatty acids and proteins from marine cold adapted microalgae for biotechnology |
publishDate |
2019 |
url |
https://research.wur.nl/en/publications/fatty-acids-and-proteins-from-marine-cold-adapted-microalgae-for- https://doi.org/10.1016/j.algal.2019.101604 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Antarc* Antarctica Arctic |
genre_facet |
Antarc* Antarctica Arctic |
op_source |
Algal Research 42 (2019) ISSN: 2211-9264 |
op_relation |
https://edepot.wur.nl/497963 https://research.wur.nl/en/publications/fatty-acids-and-proteins-from-marine-cold-adapted-microalgae-for- doi:10.1016/j.algal.2019.101604 |
op_rights |
info:eu-repo/semantics/restrictedAccess Wageningen University & Research |
op_doi |
https://doi.org/10.1016/j.algal.2019.101604 |
container_title |
Algal Research |
container_volume |
42 |
container_start_page |
101604 |
_version_ |
1788063589468209152 |