Growth and LC-PUFA production of the cold-adapted microalga Koliella antarctica in photobioreactors
Microalgae are excellent sources of polyunsaturated fatty acids (PUFAs), but only a few species have been thoroughly investigated in controlled photobioreactor conditions. In this work, the cold-adapted microalga Koliella antarctica (Trebouxiophyceae) was cultivated at 15 °C to optimize growth and P...
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Online Access: | https://research.wur.nl/en/publications/growth-and-lc-pufa-production-of-the-cold-adapted-microalga-kolie https://doi.org/10.1007/s10811-018-1606-z |
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ftunivwagenin:oai:library.wur.nl:wurpubs/541500 2024-01-14T10:02:14+01:00 Growth and LC-PUFA production of the cold-adapted microalga Koliella antarctica in photobioreactors Suzuki, Hirono Hulatt, Chris J. Wijffels, René H. Kiron, Viswanath 2019 application/pdf https://research.wur.nl/en/publications/growth-and-lc-pufa-production-of-the-cold-adapted-microalga-kolie https://doi.org/10.1007/s10811-018-1606-z en eng https://edepot.wur.nl/460892 https://research.wur.nl/en/publications/growth-and-lc-pufa-production-of-the-cold-adapted-microalga-kolie doi:10.1007/s10811-018-1606-z info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ Wageningen University & Research Journal of Applied Phycology 31 (2019) 2 ISSN: 0921-8971 Cold-adapted microalgae Eicosapentaenoic acid (EPA) Flat-plate photobioreactor LC-PUFA Omega-3 fatty acid Triacylglycerol info:eu-repo/semantics/article Article/Letter to editor info:eu-repo/semantics/publishedVersion 2019 ftunivwagenin https://doi.org/10.1007/s10811-018-1606-z 2023-12-20T23:16:02Z Microalgae are excellent sources of polyunsaturated fatty acids (PUFAs), but only a few species have been thoroughly investigated in controlled photobioreactor conditions. In this work, the cold-adapted microalga Koliella antarctica (Trebouxiophyceae) was cultivated at 15 °C to optimize growth and PUFA production in bubble-tube and flat-plate photobioreactors. The impact of nitrogen starvation, phosphorus starvation, salinity, and light intensity on the growth, fatty acid, and protein content was investigated. After culture optimization, a maximum biomass productivity of 2.37 g L−1 day−1 and maximum cell density of 11.68 g L−1 were achieved. Among all conditions tested, the maximum total fatty acid (TFA) content measured 271.9 mg g−1 dry weight in the late stationary phase. Nitrogen and phosphorus starvation strongly induced neutral lipid (TAG) accumulation, up to 90.3% of TFA, which mostly consisted of the monounsaturated fatty acid C18:1n−9 (oleic acid, OA). PUFAs were also abundant and together accounted for 30.3–45.8% of total triacylglycerol (TAG). The highest eicosapentaenoic acid (EPA) content (C20:5n−3) amounted to 6.7 mg g−1 dry weight (4.9% TFA) in control treatments, while the highest arachidonic acid (ARA) content (C20:4n−6) was 9.6 mg g−1 dry weight (3.5% TFA) in the late stationary phase. Phosphorus starvation was an effective strategy to obtain high total fatty acid yields (mg L−1) while maintaining the protein, total PUFA, and omega-3 fatty acid contents. Article in Journal/Newspaper Antarc* Antarctica Wageningen UR (University & Research Centre): Digital Library Journal of Applied Phycology 31 2 981 997 |
institution |
Open Polar |
collection |
Wageningen UR (University & Research Centre): Digital Library |
op_collection_id |
ftunivwagenin |
language |
English |
topic |
Cold-adapted microalgae Eicosapentaenoic acid (EPA) Flat-plate photobioreactor LC-PUFA Omega-3 fatty acid Triacylglycerol |
spellingShingle |
Cold-adapted microalgae Eicosapentaenoic acid (EPA) Flat-plate photobioreactor LC-PUFA Omega-3 fatty acid Triacylglycerol Suzuki, Hirono Hulatt, Chris J. Wijffels, René H. Kiron, Viswanath Growth and LC-PUFA production of the cold-adapted microalga Koliella antarctica in photobioreactors |
topic_facet |
Cold-adapted microalgae Eicosapentaenoic acid (EPA) Flat-plate photobioreactor LC-PUFA Omega-3 fatty acid Triacylglycerol |
description |
Microalgae are excellent sources of polyunsaturated fatty acids (PUFAs), but only a few species have been thoroughly investigated in controlled photobioreactor conditions. In this work, the cold-adapted microalga Koliella antarctica (Trebouxiophyceae) was cultivated at 15 °C to optimize growth and PUFA production in bubble-tube and flat-plate photobioreactors. The impact of nitrogen starvation, phosphorus starvation, salinity, and light intensity on the growth, fatty acid, and protein content was investigated. After culture optimization, a maximum biomass productivity of 2.37 g L−1 day−1 and maximum cell density of 11.68 g L−1 were achieved. Among all conditions tested, the maximum total fatty acid (TFA) content measured 271.9 mg g−1 dry weight in the late stationary phase. Nitrogen and phosphorus starvation strongly induced neutral lipid (TAG) accumulation, up to 90.3% of TFA, which mostly consisted of the monounsaturated fatty acid C18:1n−9 (oleic acid, OA). PUFAs were also abundant and together accounted for 30.3–45.8% of total triacylglycerol (TAG). The highest eicosapentaenoic acid (EPA) content (C20:5n−3) amounted to 6.7 mg g−1 dry weight (4.9% TFA) in control treatments, while the highest arachidonic acid (ARA) content (C20:4n−6) was 9.6 mg g−1 dry weight (3.5% TFA) in the late stationary phase. Phosphorus starvation was an effective strategy to obtain high total fatty acid yields (mg L−1) while maintaining the protein, total PUFA, and omega-3 fatty acid contents. |
format |
Article in Journal/Newspaper |
author |
Suzuki, Hirono Hulatt, Chris J. Wijffels, René H. Kiron, Viswanath |
author_facet |
Suzuki, Hirono Hulatt, Chris J. Wijffels, René H. Kiron, Viswanath |
author_sort |
Suzuki, Hirono |
title |
Growth and LC-PUFA production of the cold-adapted microalga Koliella antarctica in photobioreactors |
title_short |
Growth and LC-PUFA production of the cold-adapted microalga Koliella antarctica in photobioreactors |
title_full |
Growth and LC-PUFA production of the cold-adapted microalga Koliella antarctica in photobioreactors |
title_fullStr |
Growth and LC-PUFA production of the cold-adapted microalga Koliella antarctica in photobioreactors |
title_full_unstemmed |
Growth and LC-PUFA production of the cold-adapted microalga Koliella antarctica in photobioreactors |
title_sort |
growth and lc-pufa production of the cold-adapted microalga koliella antarctica in photobioreactors |
publishDate |
2019 |
url |
https://research.wur.nl/en/publications/growth-and-lc-pufa-production-of-the-cold-adapted-microalga-kolie https://doi.org/10.1007/s10811-018-1606-z |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Journal of Applied Phycology 31 (2019) 2 ISSN: 0921-8971 |
op_relation |
https://edepot.wur.nl/460892 https://research.wur.nl/en/publications/growth-and-lc-pufa-production-of-the-cold-adapted-microalga-kolie doi:10.1007/s10811-018-1606-z |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ Wageningen University & Research |
op_doi |
https://doi.org/10.1007/s10811-018-1606-z |
container_title |
Journal of Applied Phycology |
container_volume |
31 |
container_issue |
2 |
container_start_page |
981 |
op_container_end_page |
997 |
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1788057159354810368 |