Brucella pinnipedialis in grey seals (Halichoerus grypus) and harbor seals (Phoca vitulina) in the Netherlands
Brucellosis is a zoonotic disease with terrestrial or marine wildlife animals as potential reservoirs for the disease in livestock and human populations. The primary aim of this study was to assess the presence of Brucella pinnipedialis in marine mammals living along the Dutch coast and to observe a...
Published in: | Journal of Wildlife Diseases |
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Online Access: | https://research.wur.nl/en/publications/brucella-pinnipedialis-in-grey-seals-halichoerus-grypus-and-harbo https://doi.org/10.7589/2017-05-097 |
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ftunivwagenin:oai:library.wur.nl:wurpubs/539515 2024-01-14T10:09:57+01:00 Brucella pinnipedialis in grey seals (Halichoerus grypus) and harbor seals (Phoca vitulina) in the Netherlands Kroese, Michiel V. Beckers, Lisa Bisselink, Yvette J.W.M. Brasseur, Sophie van Tulden, Peter W. Koene, Miriam G.J. Roest, Hendrik I.J. Ruuls, Robin C. Backer, Jantien A. Ijzer, Jooske van der Giessen, Joke W.B. Willemsen, Peter T.J. 2018 application/pdf https://research.wur.nl/en/publications/brucella-pinnipedialis-in-grey-seals-halichoerus-grypus-and-harbo https://doi.org/10.7589/2017-05-097 en eng https://edepot.wur.nl/456838 https://research.wur.nl/en/publications/brucella-pinnipedialis-in-grey-seals-halichoerus-grypus-and-harbo doi:10.7589/2017-05-097 info:eu-repo/semantics/restrictedAccess Wageningen University & Research Journal of Wildlife Diseases 54 (2018) 3 ISSN: 0090-3558 Brucella pinnipedialis Halichoerus grypus MALDI-TOF MS MLST MLVA-16 Marine mammals Phoca vitulina The Netherlands info:eu-repo/semantics/article Article/Letter to editor info:eu-repo/semantics/publishedVersion 2018 ftunivwagenin https://doi.org/10.7589/2017-05-097 2023-12-20T23:16:55Z Brucellosis is a zoonotic disease with terrestrial or marine wildlife animals as potential reservoirs for the disease in livestock and human populations. The primary aim of this study was to assess the presence of Brucella pinnipedialis in marine mammals living along the Dutch coast and to observe a possible correlation between the presence of B. pinnipedialis and accompanying pathology found in infected animals. The overall prevalence of Brucella spp. antibodies in sera from healthy wild grey seals (Halichoerus grypus; n=11) and harbor seals (Phoca vitulina; n=40), collected between 2007 and 2013 ranged from 25% to 43%. Additionally, tissue samples of harbor seals collected along the Dutch shores between 2009 and 2012, were tested for the presence of Brucella spp. In total, 77% (30/ 39) seals were found to be positive for Brucella by IS711 real-time PCR in one or more tissue samples, including pulmonary nematodes. Viable Brucella was cultured from 40% (12/30) real-time PCR-positive seals, and was isolated from liver, lung, pulmonary lymph node, pulmonary nematode, or spleen, but not from any PCR-negative seals. Tissue samples from lung and pulmonary lymph nodes were the main source of viable Brucella bacteria. All isolates were typed as B. pinnipedialis by multiple-locus variable number of tandem repeats analysis-16 clustering and matrix-assisted laser desorption ionization-time of flight mass spectrometry, and of sequence type ST25 by multilocus sequence typing analysis. No correlation was observed between Brucella infection and pathology. This report displays the isolation and identification of B. pinnipedialis in marine mammals in the Dutch part of the Atlantic Ocean. Article in Journal/Newspaper Phoca vitulina Wageningen UR (University & Research Centre): Digital Library Journal of Wildlife Diseases 54 3 439 |
institution |
Open Polar |
collection |
Wageningen UR (University & Research Centre): Digital Library |
op_collection_id |
ftunivwagenin |
language |
English |
topic |
Brucella pinnipedialis Halichoerus grypus MALDI-TOF MS MLST MLVA-16 Marine mammals Phoca vitulina The Netherlands |
spellingShingle |
Brucella pinnipedialis Halichoerus grypus MALDI-TOF MS MLST MLVA-16 Marine mammals Phoca vitulina The Netherlands Kroese, Michiel V. Beckers, Lisa Bisselink, Yvette J.W.M. Brasseur, Sophie van Tulden, Peter W. Koene, Miriam G.J. Roest, Hendrik I.J. Ruuls, Robin C. Backer, Jantien A. Ijzer, Jooske van der Giessen, Joke W.B. Willemsen, Peter T.J. Brucella pinnipedialis in grey seals (Halichoerus grypus) and harbor seals (Phoca vitulina) in the Netherlands |
topic_facet |
Brucella pinnipedialis Halichoerus grypus MALDI-TOF MS MLST MLVA-16 Marine mammals Phoca vitulina The Netherlands |
description |
Brucellosis is a zoonotic disease with terrestrial or marine wildlife animals as potential reservoirs for the disease in livestock and human populations. The primary aim of this study was to assess the presence of Brucella pinnipedialis in marine mammals living along the Dutch coast and to observe a possible correlation between the presence of B. pinnipedialis and accompanying pathology found in infected animals. The overall prevalence of Brucella spp. antibodies in sera from healthy wild grey seals (Halichoerus grypus; n=11) and harbor seals (Phoca vitulina; n=40), collected between 2007 and 2013 ranged from 25% to 43%. Additionally, tissue samples of harbor seals collected along the Dutch shores between 2009 and 2012, were tested for the presence of Brucella spp. In total, 77% (30/ 39) seals were found to be positive for Brucella by IS711 real-time PCR in one or more tissue samples, including pulmonary nematodes. Viable Brucella was cultured from 40% (12/30) real-time PCR-positive seals, and was isolated from liver, lung, pulmonary lymph node, pulmonary nematode, or spleen, but not from any PCR-negative seals. Tissue samples from lung and pulmonary lymph nodes were the main source of viable Brucella bacteria. All isolates were typed as B. pinnipedialis by multiple-locus variable number of tandem repeats analysis-16 clustering and matrix-assisted laser desorption ionization-time of flight mass spectrometry, and of sequence type ST25 by multilocus sequence typing analysis. No correlation was observed between Brucella infection and pathology. This report displays the isolation and identification of B. pinnipedialis in marine mammals in the Dutch part of the Atlantic Ocean. |
format |
Article in Journal/Newspaper |
author |
Kroese, Michiel V. Beckers, Lisa Bisselink, Yvette J.W.M. Brasseur, Sophie van Tulden, Peter W. Koene, Miriam G.J. Roest, Hendrik I.J. Ruuls, Robin C. Backer, Jantien A. Ijzer, Jooske van der Giessen, Joke W.B. Willemsen, Peter T.J. |
author_facet |
Kroese, Michiel V. Beckers, Lisa Bisselink, Yvette J.W.M. Brasseur, Sophie van Tulden, Peter W. Koene, Miriam G.J. Roest, Hendrik I.J. Ruuls, Robin C. Backer, Jantien A. Ijzer, Jooske van der Giessen, Joke W.B. Willemsen, Peter T.J. |
author_sort |
Kroese, Michiel V. |
title |
Brucella pinnipedialis in grey seals (Halichoerus grypus) and harbor seals (Phoca vitulina) in the Netherlands |
title_short |
Brucella pinnipedialis in grey seals (Halichoerus grypus) and harbor seals (Phoca vitulina) in the Netherlands |
title_full |
Brucella pinnipedialis in grey seals (Halichoerus grypus) and harbor seals (Phoca vitulina) in the Netherlands |
title_fullStr |
Brucella pinnipedialis in grey seals (Halichoerus grypus) and harbor seals (Phoca vitulina) in the Netherlands |
title_full_unstemmed |
Brucella pinnipedialis in grey seals (Halichoerus grypus) and harbor seals (Phoca vitulina) in the Netherlands |
title_sort |
brucella pinnipedialis in grey seals (halichoerus grypus) and harbor seals (phoca vitulina) in the netherlands |
publishDate |
2018 |
url |
https://research.wur.nl/en/publications/brucella-pinnipedialis-in-grey-seals-halichoerus-grypus-and-harbo https://doi.org/10.7589/2017-05-097 |
genre |
Phoca vitulina |
genre_facet |
Phoca vitulina |
op_source |
Journal of Wildlife Diseases 54 (2018) 3 ISSN: 0090-3558 |
op_relation |
https://edepot.wur.nl/456838 https://research.wur.nl/en/publications/brucella-pinnipedialis-in-grey-seals-halichoerus-grypus-and-harbo doi:10.7589/2017-05-097 |
op_rights |
info:eu-repo/semantics/restrictedAccess Wageningen University & Research |
op_doi |
https://doi.org/10.7589/2017-05-097 |
container_title |
Journal of Wildlife Diseases |
container_volume |
54 |
container_issue |
3 |
container_start_page |
439 |
_version_ |
1788064536663687168 |