Campylobacter blaseri sp. Nov., isolated from common seals (Phoca vitulina)
During a study to assess the faecal microbiome of common seals (Phoca vitulina) in a Dutch seal rehabilitation centre, 16S rRNA gene sequences of an unknown Campylobacter taxon were identified. Campylobacter isolates, which differed from the established Campylobacter taxa, were cultured and their ta...
Published in: | International Journal of Systematic and Evolutionary Microbiology |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2018
|
Subjects: | |
Online Access: | https://research.wur.nl/en/publications/campylobacter-blaseri-sp-nov-isolated-from-common-seals-phoca-vit https://doi.org/10.1099/ijsem.0.002742 |
id |
ftunivwagenin:oai:library.wur.nl:wurpubs/537778 |
---|---|
record_format |
openpolar |
spelling |
ftunivwagenin:oai:library.wur.nl:wurpubs/537778 2024-01-28T10:05:13+01:00 Campylobacter blaseri sp. Nov., isolated from common seals (Phoca vitulina) Gilbert, Maarten J. Zomer, Aldert L. Timmerman, Arjen J. Spaninks, Mirlin P. Rubio-García, Ana Rossen, John W. Duim, Birgitta Wagenaar, Jaap A. 2018 application/pdf https://research.wur.nl/en/publications/campylobacter-blaseri-sp-nov-isolated-from-common-seals-phoca-vit https://doi.org/10.1099/ijsem.0.002742 en eng https://edepot.wur.nl/450885 https://research.wur.nl/en/publications/campylobacter-blaseri-sp-nov-isolated-from-common-seals-phoca-vit doi:10.1099/ijsem.0.002742 info:eu-repo/semantics/restrictedAccess Wageningen University & Research International Journal of Systematic and Evolutionary Microbiology 68 (2018) 5 ISSN: 1466-5026 Average nucleotide identity Campylobacter Common seal Core genome phylogeny Microbiome Novel species info:eu-repo/semantics/article Article/Letter to editor info:eu-repo/semantics/publishedVersion 2018 ftunivwagenin https://doi.org/10.1099/ijsem.0.002742 2024-01-03T23:15:22Z During a study to assess the faecal microbiome of common seals (Phoca vitulina) in a Dutch seal rehabilitation centre, 16S rRNA gene sequences of an unknown Campylobacter taxon were identified. Campylobacter isolates, which differed from the established Campylobacter taxa, were cultured and their taxonomic position was determined by a polyphasic study based on ten isolates. The isolates were characterized by 16S rRNA and atpA gene sequence analyses and by conventional phenotypic testing. Based on the whole genome sequences, the average nucleotide identity and core genome phylogeny were determined. The isolates formed a separate phylogenetic clade, divergent from all other Campylobacter taxa and most closely related to Campylobacter corcagiensis, Campylobacter geochelonis and Campylobacter ureolyticus. The isolates can be distinguished phenotypically from all other Campylobacter taxa based on their lack of motility, growth at 25 °C and growth on MacConkey agar. This study shows that these isolates represent a novel species within the genus Campylobacter, for which the name Campylobacter blaseri sp. nov. is proposed. The type strain for this novel species is 17S00004-5T (=LMG 30333T=CCUG 71276T). Article in Journal/Newspaper common seal Phoca vitulina Wageningen UR (University & Research Centre): Digital Library International Journal of Systematic and Evolutionary Microbiology 68 5 1787 1794 |
institution |
Open Polar |
collection |
Wageningen UR (University & Research Centre): Digital Library |
op_collection_id |
ftunivwagenin |
language |
English |
topic |
Average nucleotide identity Campylobacter Common seal Core genome phylogeny Microbiome Novel species |
spellingShingle |
Average nucleotide identity Campylobacter Common seal Core genome phylogeny Microbiome Novel species Gilbert, Maarten J. Zomer, Aldert L. Timmerman, Arjen J. Spaninks, Mirlin P. Rubio-García, Ana Rossen, John W. Duim, Birgitta Wagenaar, Jaap A. Campylobacter blaseri sp. Nov., isolated from common seals (Phoca vitulina) |
topic_facet |
Average nucleotide identity Campylobacter Common seal Core genome phylogeny Microbiome Novel species |
description |
During a study to assess the faecal microbiome of common seals (Phoca vitulina) in a Dutch seal rehabilitation centre, 16S rRNA gene sequences of an unknown Campylobacter taxon were identified. Campylobacter isolates, which differed from the established Campylobacter taxa, were cultured and their taxonomic position was determined by a polyphasic study based on ten isolates. The isolates were characterized by 16S rRNA and atpA gene sequence analyses and by conventional phenotypic testing. Based on the whole genome sequences, the average nucleotide identity and core genome phylogeny were determined. The isolates formed a separate phylogenetic clade, divergent from all other Campylobacter taxa and most closely related to Campylobacter corcagiensis, Campylobacter geochelonis and Campylobacter ureolyticus. The isolates can be distinguished phenotypically from all other Campylobacter taxa based on their lack of motility, growth at 25 °C and growth on MacConkey agar. This study shows that these isolates represent a novel species within the genus Campylobacter, for which the name Campylobacter blaseri sp. nov. is proposed. The type strain for this novel species is 17S00004-5T (=LMG 30333T=CCUG 71276T). |
format |
Article in Journal/Newspaper |
author |
Gilbert, Maarten J. Zomer, Aldert L. Timmerman, Arjen J. Spaninks, Mirlin P. Rubio-García, Ana Rossen, John W. Duim, Birgitta Wagenaar, Jaap A. |
author_facet |
Gilbert, Maarten J. Zomer, Aldert L. Timmerman, Arjen J. Spaninks, Mirlin P. Rubio-García, Ana Rossen, John W. Duim, Birgitta Wagenaar, Jaap A. |
author_sort |
Gilbert, Maarten J. |
title |
Campylobacter blaseri sp. Nov., isolated from common seals (Phoca vitulina) |
title_short |
Campylobacter blaseri sp. Nov., isolated from common seals (Phoca vitulina) |
title_full |
Campylobacter blaseri sp. Nov., isolated from common seals (Phoca vitulina) |
title_fullStr |
Campylobacter blaseri sp. Nov., isolated from common seals (Phoca vitulina) |
title_full_unstemmed |
Campylobacter blaseri sp. Nov., isolated from common seals (Phoca vitulina) |
title_sort |
campylobacter blaseri sp. nov., isolated from common seals (phoca vitulina) |
publishDate |
2018 |
url |
https://research.wur.nl/en/publications/campylobacter-blaseri-sp-nov-isolated-from-common-seals-phoca-vit https://doi.org/10.1099/ijsem.0.002742 |
genre |
common seal Phoca vitulina |
genre_facet |
common seal Phoca vitulina |
op_source |
International Journal of Systematic and Evolutionary Microbiology 68 (2018) 5 ISSN: 1466-5026 |
op_relation |
https://edepot.wur.nl/450885 https://research.wur.nl/en/publications/campylobacter-blaseri-sp-nov-isolated-from-common-seals-phoca-vit doi:10.1099/ijsem.0.002742 |
op_rights |
info:eu-repo/semantics/restrictedAccess Wageningen University & Research |
op_doi |
https://doi.org/10.1099/ijsem.0.002742 |
container_title |
International Journal of Systematic and Evolutionary Microbiology |
container_volume |
68 |
container_issue |
5 |
container_start_page |
1787 |
op_container_end_page |
1794 |
_version_ |
1789331402727096320 |